Histology

组织学

• 批准号: 10617308
• 负责人: Omer Yilmaz
• 金额: $ 25.53万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-17 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10617308
• 关键词: Bioinformatics; Biological Markers; Budgets; Cancer Center Support Grant; Cells; Chromosome Mapping; Collaborations; Communities; Computer software; Consultations; Development; Devices; Diagnosis; Diagnostic; Drug Delivery Systems; Education; Educational workshop; Equipment; Event; Experimental Designs; Funding; Gene Expression; Genes; Genomics; Histologic; Histology; Image Analysis; Immune response; Immunohistochemistry; Information Centers; International; Malignant Neoplasms; Methodology; Methods; Microtome - medical device; Mission; Molecular; Monitor; Mus; Mutation; Neoplasm Metastasis; Oncogenes; Paraffin; Pathologist; Pathology; Pathway interactions; Pharmaceutical Preparations; Phenotype; Preparation; Prevention; Protocols documentation; Research; Research Personnel; Research Support; Resource Sharing; Resources; Role; Sampling; Scanning; Services; Slide; Source; Stains; Technology; Therapeutic; Tissues; Training; Tumorigenicity; Update; Zebrafish; digital; digital imaging; drug efficacy; efficacy evaluation; in vivo; instrumentation; investigator training; member; model organism; nanomaterials; nanoparticle; neoplasm immunotherapy; programs; response; sample collection; success; therapeutic target; tool; training opportunity; treatment response; tumor; tumor diagnosis; tumor initiation; tumorigenesis; vaccine delivery

Histology Core: Project Summary/Abstract Koch Institute Members use vertebrate model organisms as a key tool to study the role of known or putative cancer genes in development and tumorigenicity; investigate the mechanisms of tumor initiation, progression and metastasis; evaluate the role of stroma and immune responses in tumorigenesis; and assess the efficacy of drugs, nanomaterials and devices in therapeutic and diagnostic applications. Thus, it is essential that Center Members are able to correctly diagnose developmental and tumor phenotypes, evaluate the underlying molecular events, and accurately and quantitatively assess drug or vaccine delivery, localized tissue response, and overall therapeutic response. The Koch Institute Histology Core is a Shared Resource that provides state- of-the-art histological services to support these studies. This includes assistance and/or training in tissue sectioning, slide preparation, staining and analysis, and access to the consultative services of an internationally recognized Veterinary Pathologist, Dr. Roderick Bronson, for diagnosis of tumor and tissue phenotypes. During the current period, usage of the Histology Core increased from 67% to 77% of Center Members, and included investigators from all three Programs. In response to Center member needs, the Histology Core expanded services and instrumentation. This includes expanded offerings for immunohistochemistry and special stains, and investigator training for independent sectioning. The Core also acquired new digital slide scanning capabilities and quantification software, and made upgrades to existing key instrumentation. Thus, this Shared Resource is essential to the success of the Koch Institute mission. In the upcoming period, The Histology Core is committed to offering a wide range of state-of-the-art histological services to support the research programs of Center Members. Planned initiatives include: working with the Integrated Genomics & Bioinformatics Core to establish methodologies for mapping gene expression at the single cell level within tissues; developing expertise and providing guidance in use of automated AI-driven analysis packages (such as QuPath) for analysis of histological sections; evaluating instrumentation to determine community benefit for fluorescent digital slide scanning; and expanding histology training offerings to include more hands-on workshop opportunities. This shared Core is of exceptional value to the CCSG because Koch Institute Members account for 95% of the Core services usage. Notably, the requested CCSG budget for Year 49 is 6.8% less than the budget in the current period (Year 48).

组织学核心：项目摘要/摘要 科赫研究所成员使用脊椎动物模型生物作为研究已知或假定的作用的关键工具 发育和致瘤性中的癌症基因；研究肿瘤发生、发展的机制 和转移；评估基质和免疫反应在肿瘤发生中的作用；并评估疗效 治疗和诊断应用中的药物、纳米材料和设备。因此，中心非常有必要 成员能够正确诊断发育和肿瘤表型，评估潜在的 分子事件，并准确定量地评估药物或疫苗的递送、局部组织反应、 和总体治疗反应。科赫研究所组织学核心是一个共享资源，提供状态- 最先进的组织学服务来支持这些研究。这包括组织方面的帮助和/或培训 切片、载玻片制备、染色和分析，以及获得咨询服务 国际公认的兽医病理学家 Roderick Bronson 博士负责肿瘤和组织的诊断 表型。 当前期间，中心成员对组织学核心的使用率从 67% 增加到 77%，并且 包括来自所有三个计划的研究人员。为了满足中心成员的需求，组织学核心 扩大服务和仪器。这包括扩大免疫组织化学产品和 特殊染色和独立切片的研究者培训。 The Core 还购买了新的数字幻灯片 扫描能力和量化软件，并对现有关键仪器进行升级。因此， 这种共享资源对于科赫研究所使命的成功至关重要。 在接下来的时期，组织学核心致力于提供广泛的最先进的组织学 支持中心成员研究计划的服务。计划的举措包括： 集成基因组学和生物信息学核心，建立绘制基因表达图谱的方法 组织内的单细胞水平；开发专业知识并提供使用自动化人工智能驱动的指导 用于组织切片分析的分析包（例如 QuPath）；评估仪器 确定荧光数字载玻片扫描的社区效益；并将组织学培训课程扩展到 包括更多实践研讨会的机会。这个共享核心对 CCSG 具有非凡的价值，因为 科赫研究所会员占核心服务使用量的 95%。值得注意的是，所请求的 CCSG 预算 第 49 年比本期（第 48 年）预算少 6.8%。