Histology

组织学

• 批准号: 10617308
• 负责人: Omer Yilmaz
• 金额: $ 25.53万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-17 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10617308
• 关键词: Bioinformatics; Biological Markers; Budgets; Cancer Center Support Grant; Cells; Chromosome Mapping; Collaborations; Communities; Computer software; Consultations; Development; Devices; Diagnosis; Diagnostic; Drug Delivery Systems; Education; Educational workshop; Equipment; Event; Experimental Designs; Funding; Gene Expression; Genes; Genomics; Histologic; Histology; Image Analysis; Immune response; Immunohistochemistry; Information Centers; International; Malignant Neoplasms; Methodology; Methods; Microtome - medical device; Mission; Molecular; Monitor; Mus; Mutation; Neoplasm Metastasis; Oncogenes; Paraffin; Pathologist; Pathology; Pathway interactions; Pharmaceutical Preparations; Phenotype; Preparation; Prevention; Protocols documentation; Research; Research Personnel; Research Support; Resource Sharing; Resources; Role; Sampling; Scanning; Services; Slide; Source; Stains; Technology; Therapeutic; Tissues; Training; Tumorigenicity; Update; Zebrafish; digital; digital imaging; drug efficacy; efficacy evaluation; in vivo; instrumentation; investigator training; member; model organism; nanomaterials; nanoparticle; neoplasm immunotherapy; programs; response; sample collection; success; therapeutic target; tool; training opportunity; treatment response; tumor; tumor diagnosis; tumor initiation; tumorigenesis; vaccine delivery

Histology Core: Project Summary/Abstract Koch Institute Members use vertebrate model organisms as a key tool to study the role of known or putative cancer genes in development and tumorigenicity; investigate the mechanisms of tumor initiation, progression and metastasis; evaluate the role of stroma and immune responses in tumorigenesis; and assess the efficacy of drugs, nanomaterials and devices in therapeutic and diagnostic applications. Thus, it is essential that Center Members are able to correctly diagnose developmental and tumor phenotypes, evaluate the underlying molecular events, and accurately and quantitatively assess drug or vaccine delivery, localized tissue response, and overall therapeutic response. The Koch Institute Histology Core is a Shared Resource that provides state- of-the-art histological services to support these studies. This includes assistance and/or training in tissue sectioning, slide preparation, staining and analysis, and access to the consultative services of an internationally recognized Veterinary Pathologist, Dr. Roderick Bronson, for diagnosis of tumor and tissue phenotypes. During the current period, usage of the Histology Core increased from 67% to 77% of Center Members, and included investigators from all three Programs. In response to Center member needs, the Histology Core expanded services and instrumentation. This includes expanded offerings for immunohistochemistry and special stains, and investigator training for independent sectioning. The Core also acquired new digital slide scanning capabilities and quantification software, and made upgrades to existing key instrumentation. Thus, this Shared Resource is essential to the success of the Koch Institute mission. In the upcoming period, The Histology Core is committed to offering a wide range of state-of-the-art histological services to support the research programs of Center Members. Planned initiatives include: working with the Integrated Genomics & Bioinformatics Core to establish methodologies for mapping gene expression at the single cell level within tissues; developing expertise and providing guidance in use of automated AI-driven analysis packages (such as QuPath) for analysis of histological sections; evaluating instrumentation to determine community benefit for fluorescent digital slide scanning; and expanding histology training offerings to include more hands-on workshop opportunities. This shared Core is of exceptional value to the CCSG because Koch Institute Members account for 95% of the Core services usage. Notably, the requested CCSG budget for Year 49 is 6.8% less than the budget in the current period (Year 48).

组织学核心：项目摘要/摘要 科赫研究所成员使用脊椎动物模型生物作为研究已知或推定的作用的关键工具 发育和肿瘤性的癌症基因；研究肿瘤起始，进展的机制 和转移；评估基质和免疫反应在肿瘤发生中的作用；并评估功效 治疗和诊断应用中的药物，纳米材料和装置的因此，至关重要的是中心 成员能够正确诊断发育和肿瘤表型，评估基础 分子事件，准确，定量评估药物或疫苗的递送，局部组织反应， 和整体治疗反应。科赫研究所组织学核心是一种共享资源，可提供国家 - 组织学服务以支持这些研究。这包括在组织中的帮助和/或培训 切片，幻灯片准备，染色和分析，以及访问 国际认可的兽医病理学家罗德里克·布朗森（Roderick Bronson）博士诊断肿瘤和组织 表型。 在当前时期，组织学核心的使用从中心成员的67％增加到77％， 包括来自所有三个计划的调查人员。为了响应中心成员需求，组织学核心 扩展的服务和仪器。这包括扩大免疫组织化学和 特殊污渍和研究人员培训进行独立分区。核心还获得了新的数字幻灯片 扫描功能和量化软件，并升级到现有的关键仪器。因此， 这种共享资源对于科赫研究所任务的成功至关重要。 在接下来的时期，组织学核心致力于提供广泛的最新组织学 支持中心成员研究计划的服务。计划的举措包括：与 综合基因组学和生物信息学核心，以建立在映射基因表达的方法论 组织内的单细胞水平；开发专业知识并提供使用自动AI驱动的指导 分析软件包（例如Qupath）用于分析组织学部分；评估仪器 确定荧光数字幻灯片扫描的社区利益；并扩大组织学培训产品 包括更多动手研讨会的机会。此共享核心对CCSG具有非凡的价值 Koch Institute成员占核心服务使用情况的95％。值得注意的是，要求的CCSG预算 49年的预算比目前的预算低6.8％（48年）。