Pilot Project Research Core

试点项目研究核心

• 批准号: 10626093
• 负责人: Stephen Leigh Cowen
• 金额: $ 13.51万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2026-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10626093
• 关键词: Advisory Committees; Anesthesia procedures; Animal Behavior; Animals; Arizona; Attention; Behavior; Behavioral; Budgets; Cognitive; Collection; Communication; Conflict of Interest; Core Facility; Corpus striatum structure; Cues; Data; Dedications; Development; Devices; Dopamine; Drug Addiction; Drug abuse; Electronic Mail; Emotional; Exhibits; Exposure to; Extramural Activities; Feedback; Fostering; Funding; Future; Genetic; Goals; Grant; Impairment; Individual; Learning; Letters; Measurement; Methodology; Methods; Molecular; Mus; National Institute of Drug Abuse; Neural Inhibition; Neurology; Neuronal Plasticity; Neurons; Neurosciences; Opioid; Pain; Pharmaceutical Preparations; Pharmacology; Phase; Photometry; Physiological; Physiology; Pilot Projects; Play; Psychology; Relapse; Research; Research Personnel; Research Project Grants; Resources; Role; Scientist; Services; Substance abuse problem; Summary Reports; System; Technology; Testing; United States National Institutes of Health; Universities; Wireless Technology; Work; Writing; addiction; awake; behavioral study; chronic pain; cognitive testing; executive function; experimental study; flexibility; improved; innovation; interest; meetings; neural; neural circuit; neuroregulation; new technology; novel strategies; opioid exposure; optogenetics; pain reduction; programs; service utilization; theories; wasting; web site; wireless

Summary Pilot Research Project Core The Pilot Research Project Core (PRP) provides grant support and advising services for scientists interested in advancing the field of substance abuse. The Pilot Core will encourage applications from early-stage investigators preparing for their first NIDA R01 proposal. The PRP will also work with applicants to match them with the many technical services offered by the Center Cores. The Pilot Core will also support scientists developing new technologies that advances addiction research. To these ends, we describe three pilot projects that are ready for support and illustrate the focus of the PRP. 1) Do interactions between chronic pain and opioid exposure reduce cognitive flexibility? Drug abuse often follows emotional and physical pain. Data from our group indicates that chronic pain reduces executive function which can contribute to relapse. We hypothesize that chronic pain and previous exposure to opioids will interact such that animals exposed to chronic pain and opioids will exhibit severely reduced executive function and show evidence for relapse. We will test this hypothesis using Behavioral Core facilities, utilizing instrumental tests of cognitive flexibility. These studies will provide key pilot data for a NIDA R01 proposal investigating interactions between addiction, chronic pain, and executive function. 2) Wireless Stimulation and Photometric Measurement of Neural Activity in Mice: Technologies for the selective stimulation/inhibition of neural subtypes are needed to establish the causal roles they play in addiction. Traditional approaches use tethers connected to the animal that disrupt behavior and impair assessment of addiction-associated behaviors. This project supports development of wireless technologies that integrate optogenetic stimulation and photometry for measurement of neural activity without disrupting natural animal behavior. We plan to use this device to stimulate and suppress neurons associated with addiction. These technologies would benefit the Analytical and Behavioral Cores by eliminating the need for tethers during measurement and stimulation. This project will use services provided by the Behavioral and Genetics Cores. 3) Simultaneous Measurement of Dopamine Release and Single-Unit Activity in Behaving Animals. Dopamine release supports learning by triggering neuroplastic changes in cortical and striatal circuits. Addiction may result from atypically large dopamine release that enhances learning of drug-associated cues. There is no direct evidence for this theory as few methods exist for the simultaneous measurement of dopamine release and single-unit activity. Our group developed such a system for use in anesthetized animals. The goal of this pilot proposal is to adapt this system for routine use in awake and behaving animals, and to determine if functional connections between neurons are enhanced by phasic dopamine release. Experiments will utilize services of the Behavioral Core and preliminary data will be used for a planned NIDA proposal.

摘要试点研究项目核心 飞行员研究项目核心（PRP）为有兴趣的科学家提供赠款支持和建议 推进药物滥用领域。飞行员核心将鼓励早期调查人员的申请 为他们的第一个NIDA R01提案做准备。 PRP还将与申请人合作，将他们与许多 中心核心提供的技术服务。飞行员核心还将支持科学家开发新的 推进成瘾研究的技术。对于这些目的，我们描述了三个准备就绪的试点项目 支持并说明PRP的重点。 1）慢性疼痛与阿片类药物暴露之间的相互作用是否会降低认知灵活性？吸毒 通常遵循情绪和身体上的痛苦。来自我们小组的数据表明，慢性疼痛会减少执行 可能有助于复发的功能。我们假设慢性疼痛和先前接触阿片类药物的疼痛将 相互作用使暴露于慢性疼痛和阿片类药物的动物将显示出严重降低的执行功能 并显示复发的证据。我们将使用行为核心设施检验该假设，并利用工具 认知灵活性的测试。这些研究将为调查NIDA R01提案提供关键的试点数据 成瘾，慢性疼痛和执行功能之间的相互作用。 2）小鼠神经活动的无线刺激和光度测量： 需要对神经亚型的选择性刺激/抑制来确定它们在成瘾中发挥的因果作用。 传统方法使用与动物相关的系数，破坏行为并损害评估 与成瘾相关的行为。该项目支持整合无线技术的开发 光遗传学刺激和光度法用于测量神经活动而不破坏天然动物 行为。我们计划使用该设备刺激和抑制与成瘾有关的神经元。这些 技术将通过消除在 测量和刺激。该项目将使用行为和遗传学核心提供的服务。 3）在行为动物中同时测量多巴胺释放和单单位活动。 多巴胺释放通过触发皮质和纹状体回路的神经塑性变化来支持学习。瘾 可能是由于非典型多巴胺释放而引起的，从而增强了与药物相关线索的学习。没有 该理论的直接证据是因为多巴胺释放的同时测量和 单单元活动。我们的小组开发了一种用于麻醉动物的系统。这位飞行员的目标 建议是为了使该系统适应醒着和行为动物的常规使用，并确定功能是否功能 神经元之间的连接通过阶段性多巴胺释放增强。实验将利用 行为核心和初步数据将用于计划中的NIDA建议。