GINGIVAL FLUID POLYAMINES AND PMN MODULATION

PMN的功能受配体与表面受体结合的调节， 诱导胞浆Ca 2+瞬时增加，动员蛋白激酶C (PKC)，并启动PMN激活。 Ca 2+信号被改变， 多胺，这是普遍存在于牙龈液在发炎牙周 网站. 我们假设多胺能显著提高钙离子浓度， PMN动员，从而调节许多重要的反应， 细胞外刺激 我们将在体外测试这一假设， 多胺在三个作用水平上的作用：1）调节 化学引诱剂诱导的Ca 2+动员，2）PKC的调节 细胞蛋白质的易位和磷酸化，和3）调节 PMN功能受Ca ~（2+）和PKC的调节。 这些研究将提供一个 更好地理解涉及局部调节的复杂机制 PMN功能在牙周病和其他炎症性疾病中的作用。

PMN function is modulated by ligand binding to surface receptors, which induces transient increases in cytosolic Ca2+ mobilizes protein kinase C (PKC), and initiates PMN activation. Ca2+ signalling is altered by polyamines, which are prevalent in gingival fluid at inflamed periodontal sites. We hypothesize that polyamines significantly enhance Ca2+ mobilization in PMN and thereby modulate many essential responses to extracellular stimuli. We will test this hypothesis in vitro by examining the effects of polyamines at three levels of action: 1) Modulation of chemoattractant-induced Ca2+ mobilization, 2) Modulation of PKC translocation and phosphorylation of cell proteins, and 3) Modulation of PMN functions regulated by Ca2+ and PKC. These studies will provide a better understanding of the complex mechanisms involved in local modulation of PMN function in periodontal disease and other inflammatory disorders.