NGF PEPTIDES AND RECEPTORS AND BPH

年第二大死亡原因和第一大致病原因 40岁以上男性为前列腺瘤(良性前列腺 增生症和腺癌)。因此，对植物生长的调控 前列腺癌已经成为卫生保健专业人员和成年人的主要担忧 一般都是男性。Daniel Djakiew博士正在申请研究职业 发展奖，以便让候选人全神贯注 与旁分泌调节有关的几个有希望的发现 前列腺生长。具体来说，候选人已经建立了一个人类 分泌一种新型神经营养因子(NGF样)的前列腺基质细胞系 肽)，其依次与低亲和力(P75)和高亲和力相互作用 (Protrk)NGF受体刺激人前列腺上皮细胞生长。 候选人的短期目标是，首先，检查 类固醇在基质细胞产生NGF多肽过程中的作用。这是 老年男性类固醇环境变化的意义 同时，前列腺癌的发病率也在增加。第二 该研究计划的目的是研究神经生长因子受体的表达 在良性前列腺增生症(BPH)组织中。值得注意的是，表达 P75神经生长因子受体在肿瘤患者中逐渐丢失 前列腺癌(前列腺增生症和腺癌)。因为p75神经生长因子受体可以调节 Protrk NGF受体的活性，P7S NGF受体的丢失可能 消除Protrk磷酸酪氨酸激酶的调制，导致 前列腺上皮细胞的不受控制的生长。在第三节 有针对性的，器官培养技术将被用来调查 NGF多肽与类固醇在调节平衡中的相互作用 在BPH组织中细胞增殖和细胞死亡之间的关系。这个 候选人的长期目标是利用这项研究的发现 作为一种手段来控制前列腺的生长 修改和/或治疗前列腺瘤。

The second leading cause of mortality and leading cause of morbidity in males above the age of 40 years is prostatic neoplasia (benign prostatic hyperplasia and adenocarcinoma). Consequently, growth regulation of the prostate has become a major concern to health care professionals and adult males in general. Dr. Daniel Djakiew is applying for a Research Career Development Award in order to allow the candidate to devote his attention to several promising discoveries pertaining to the paracrine regulation of prostate growth. Specifically, the candidate has established a human prostate stromal cell line which secretes a novel neurotrophin (NGF-like peptide) that in turn interacts with low affinity (p75) and high affinity (protrk) NGF receptors to stimulate growth of human prostate epithelia. The short term goals of the candidate are, firstly, to examine the role of steroids in the production of the NGF peptide by stroma cells. This is of significance since the steroidal milieu of the aging male changes in parallel with the increased incidence of prostatic neoplasia. The second aim of the research plan is to investigate the expression of NGF receptors in benign prostatic hyperplasia (BPH) tissues. Significantly, expression of the p75 NGF receptor is progressively lost in the neoplastic human prostate (BPH and adenocarcinoma). Since the p75 NGF receptor may modulate the activity of the protrk NGF receptor, loss of the p7S NGF receptor may eliminate modulation of the protrk phosphotyrosine kinase, leading to uncontrolled growth of the prostate epithelial cells. In the third specific aim, organ culture techniques will be utilized to investigate the interaction between the NGF peptide and steroids in modulating the balance between cell proliferation and cell death within BPH tissue. The candidate's long term goals are to utilize the findings of this research for the development of to manipulate growth of prostate as a means to modify and/or treat prostatic neoplasia.