PHOTOCHEMICAL AND MICROBIOLOGICAL FATES AND EFFECTS OF XENOBIOTIC COMPOUNDS

具体目标是：(1)测定光化学速率常数 和微生物降解选定的外来物质，如氯化或 淡水和河口环境中的硝化芳香族化合物(2) 以确定环境因素的影响，如季节、pH和 光解速率常数与微生物降解速率常数的适应 测试化合物，(3)评估测试的诱变效果 化合物及其光产物，(4)评估其适用性 细菌异养活性测定作为毒性指标的研究 水生生物中的指示物(以及它们与诱变效应的关系) 生态系统，(5)评估光解和适应的贡献 对测试化合物的微生物降解，并确定 光解和微生物降解在环境中的相对重要性 测试化合物的去除过程，(6)培训少数民族学生 进行环境和生物医学研究，以分析 实验数据，并独立报告实验结果。 该方法涉及使用同步孵化系统来 确定测试的光化学和微生物的命运 化合物。此外，测试化合物的诱变作用和 他们的照相产品将用Ames方法进行检验。这个 致突变性试验的结果将与 细菌异养活性的同步测量，以 测定后一种方法检测毒性的敏感性。 这项研究的长远目标是：(1)制作 用于监管机构预测测试持续性的数据库 并提供了更准确和快速的方法来检测 受试化合物及其光产物的毒性，(2)具有 为工业部门提供技术服务，这应该 加强产业界和杰克逊州立大学之间的关系， (三)提供了理论和实践两方面的培训 少数族裔学生毕业后能够成功竞争 与环境相关的研究生学位课程。

The specific aims are: (1) to determine rate constants of photochemical and microbial degradation of selected xenobiotics such as chlorinated or nitrated aromatic compounds in freshwater and estuarine environments, (2) to determine the effects of environmental factors such as season, pH, and adaptation on rate constants of photolysis and microbial degradation of the test compounds, (3) to assess the mutagenic effects of the test compounds and their photoproducts, (4) to assess the applicability of using measurement of bacterial heterotrophic activity as toxicity indicator (and how they relate to mutagenic effects) in aquatic ecosystems, (5) to evaluate the contribution of photolysis and adaptation to microbial degradation of the test compounds and to determine the relative importance of photolysis and microbial degradation in the removal process of the test compounds, (6) to train minority students to conduct environmental and biomedical research, to analyze the experimental data, and to report the experimental results independently. The approach involves the use of simultaneous incubation systems to determine the photochemical and microbiological fates of the test compounds. In addition, the mutagenic effects of the test compounds and their photoproducts will be examined with the method of Ames. The results of mutagenicity experiments will be compared to the results of concurrent measurements of bacterial heterotrophic activities, to determine the sensitivity of the latter method in detecting the toxicity. The long-term objectives of this study are: (1) to have produced the data base for the regulatory agencies to predict persistence of the test compounds and to have provided more accurate and rapid methods to detect the toxicity of the test compounds and their photoproducts, (2) to have provided a technical service to the industrial sector which should strengthen relationships between industry and Jackson State University, and (3) to have provided both academic and practical training for minority students who upon graduation are able to compete successfully in environmentally-related graduate degree programs.