CHARACTERIZATION OF AN HLA DW2-LIKE CELL AT THE GENOMIC LEVEL

人类主要疾病中的人类白细胞抗原-D区，或II类基因 组织相容性复合体由三个多基因亚区组成(即， Dr、dq、dp)编码一系列高度多态细胞表面 参与调节免疫反应的糖蛋白。人类白细胞抗原D区 不同民族的抗原性不同。这些抗原是 通常与高加索人中特定的DR特异性有关 在非高加索人口中可能没有人口，而不同的人口 特异度被替换。这些相关性之所以重要，是因为 发现人类白细胞抗原-D区特定等位基因与 各种人类疾病。这项提议的一个主要目标是 非洲人人类白细胞抗原-D区抗原的定义和特征 美国人口。人类白细胞抗原基因多态性的一个更好的定义 不同民族在不同地区具有重要的生物医学意义 例如器官移植的组织相容性匹配和 少数民族更多地参与民族骨髓 移植计划。这项提议的重点是基因组 人类白细胞抗原DR2样细胞的DNA限制性内切酶分析 基因座和等位基因特异的寡核苷酸探针和序列分析。 这项研究将使用上述技术来解决以下具体问题 目的：检验杂交模式是否存在差异 人类白细胞抗原-DR2亚型DNA与类DR2细胞DNA的比较 KWh与已发表的DR亚型的分布；比较等位基因 KWH体外扩增DNA结构域I的高变量序列 (类DR2细胞)和其他DR2亚型，并对kWh进行测序。

The HLA-D region, or class II genes, in the human major histocompatibility complex consists of three multigene subregions (i.e., DR, DQ, DP) that encode a highly polymorphic series of cell-surface glycoproteins involved in regulating the immune response. HLA-D region antigens vary among different ethnic groups. those antigens that are normally associated with a particular DR specificity in a caucasian population may be absent in a non-caucasian population and a different specificity substituted. These correlations are important because of the relationship found between particular alleles at the HLA-D region and a variety of human diseases. A major objective of this proposal is the definition and characterization of HLA-D region antigens in the African American population. A better definition of HLA polymorphism in different ethnic groups has important biomedical implications in areas such as histocompatibility matching for organ transplantation and the greater participation of ethnic minorities in national bone marrow transplantation programs. The focus of this proposal is the genomic analysis of a HLA-"DR2 like" cell by DNA restriction enzyme analysis, locus and allele specific oligonucleotide probes, and sequence analysis. The study will use the above techniques to address the following specific aims: to test whether there are differences in hybridization patterns between DNA from HLA-DR2 subtypes and the DR2-like cell; to compare RFLP profiles of KWH with published DR subtypes; to contrast allelic hypervariable sequences in domain I of in vitro amplified DNA from KWH (DR2-like cell) and other DR2 subtypes and to sequence KWH.