CHEMOSTERILANT ACTIVITY OFJUVENILE HORMONE ANALOG--DUGESIA OR AEDES SECRETIONS

在拟议的生物医学研究中，首席研究员和 学生们将试图中断传播、发展和 亚洲虎蚊媒介伊蚊种群增长过程的研究 美洲的白纹伊蚊(Skuse)。具体地说，长期或 最终目标是防止可能的几种 我国流行的虫媒病毒包括登革热和君越脑炎病毒 美洲白纹伊蚊，通过性释放 绝育雄虫与杀幼虫繁殖地的处理 粘液分泌物中的物质。这些方法应该能够 极大地减少了Ae.白纹伊蚊，因为没有 预防或治疗这些人类感染的治疗措施。 1980年，第一例本土登革热传播发生在 美国在35年，随后在1986年。 为达到目标，建议的研究分为五个阶段。 (1)成人口腔治疗(实验室和野外笼养研究)；(2) 幼虫对F_1代的影响(3)处理组寿命 男性，(4)男性不育的永久性和(5)粘液分泌物 (提取、测试和识别粘液中的有毒物质)。 将通过口服治疗进行消毒效果测试 成虫阶段，Ae.白纹伊蚊有三类幼虫， 苯基酚(J2419、J2644、J2706)、苯并二恶酚(J2581、J2693)和a 氨基甲酸酯(苯氧碳酸酯)。不能孵化的鸡蛋将被用作 鸡蛋诱导不育性的计算标准。杀幼虫 粘液分泌物的效果将通过一系列 不同龄期幼虫的实验室耐受性试验 分泌物的浓度水平更高。另外，粘液的样本 将对分泌物进行分析以分离和确定(通过使用 高效液相色谱法)粘液中的目标杀幼虫物质。

In the proposed biomedical research, the principal investigator and students will attempt to interrupt the dispersal, development and population growth process of the Asian tiger mosquito vector, Aedes albopictus (Skuse) in the Americas. Specifically, the long term or ultimate objective is to prevent the possible transmission of several endemic arboviruses including Dengue and LaCrosse encephalitis virus in the Americas by Aedes albopictus, through the release of sexually sterilized males and the treatment of breeding habitats with larvicidal materials from mucous secretions. These methods should be capable of drastically reducing populations of Ae. albopictus, since there is no therapeutic measure for preventing or treating these human infections. In 1980, the first indigenous transmission of dengue occurred in the United States in thirty-five years and subsequently in 1986. To obtain the objectives the proposed study is divided into five phases (1) oral treatment of adults (laboratory and field cage studies), (2) effects of juveniods on the F1 generation, (3) longevity of the treated males, (4) permanency of male sterility and (5) mucous secretions (extract, test and identify toxic substances in the mucous). Tests for chemosterilant effect will be conducted through oral treatment of the adult stage, Ae. albopictus with three classes of juveniods, benzylphenols (J2419, J2644, J2706), benzodioxoles (J2581, J2693) and a Carbamate (fenoxycarb). Non-hatchability of eggs will be used as a criterion for calculating sterility induced in eggs. The larvicidal effect of mucous secretions will be determined through a series of laboratory tolerance tests with the different larval instars at two or more concentration levels of the secretions. Also, samples of the mucous secretions will be analyzed to separate and determine (through use of HPLC) the target larvicidal material in the mucous.