SIZE AND DYNAMICS OF MICELLES

时间分辨荧光猝灭（TRFQ）测量将 可以在胶束和囊泡系统上进行， 脂质生物膜，并发现应用在药物输送和作为 生化反应中间TRFQ产生关于大小的信息 胶束的分布，添加剂的统计分布， 添加剂之间的双分子碰撞速率的动力学 胶束。 目标是（1）进行TRFQ实验， （2）应用TRFQ和 电子顺磁共振（EPR）在相同的系统和 对来自双方的数据进行相互一致的解释 实验;因此表明，使用互补的 技术，大大提高了信息的质量;（4）应用 该方法适用于磷脂胶束和囊泡的研究。 的 长期目标是有助于（1）阐明 生物膜结构，（2）基本原理的理解 （3）多学科协同研究， 化学系和生物系的同事。 在TRFQ中， 芘将用作荧光探针， 自由基作为猝灭剂（也是顺磁性分子）。 在 解释实验数据，通常认为添加剂 在胶束中随机分布。 派奖期间 实验将在一些经过充分研究的系统上进行，如 在添加剂存在下的SDS、LiDS和CTAC。 在TRFQ中 方法芘荧光的真实的时间衰减是 测定了 衰减时间缩短（淬火）， 猝灭剂作为与探针碰撞的结果。 淬火 速率常数取决于探针之间的碰撞频率 以及胶束中或胶束处的猝灭剂，这反过来取决于 胶束大小。 有一个框架， 衰减特性和猝灭速率常数 胶束的平均粒径和粒径分布。 在互补 EPR实验将由CSUN的Bales教授在 相同的系统，氮氧自由基的自旋弛豫时间将是 测定了 双分子碰撞缩短了相对时间， 碰撞频率取决于胶束尺寸。 所提取的 胶束的大小和分布依赖于统计假设 所有添加剂在胶束上的分布。 统计 将调整添加剂的分布以符合数据 从两个实验。 这两种技术在 因此，相同的系统将给出准确和可靠的确定 胶束的大小和添加剂的分布。

Time-resolved fluorescence quenching (TRFQ) measurements will be performed on systems of micelles and vesicles which are models of lipid biomembranes and find applicatins in drug delivery and as biochemical reaction medial TRFQ yields information on the size distribution of micelles, the statistical distribution of additives and the dynamics of bimolecular collision rates between additives on the micelles. The goals are (1) perform TRFQ experiments on specific systems; (2) apply the combined techniques of TRFQ and electron paramagnetic resonance (EPR) on identical systems and arrive at a mutually consistent interpretation of data from both experiments; and thus show that the use of complementary techniques, greatly improves the quality of information; (4) apply the method to the study of phospholipid micelles and vesicles. The long term goal is to contribute to (1) the elucidation of biomembrane structure, (2) the understanding of the fundamentals of self-organization; (3) a synergetic multidisciplinary research with colleagues in the Chemistry and Biology departments. In TRFQ, pyrene will function as the fluorescence probe and a nitroxide free radical as the quencher (also a paramagnetic molecule). In interpreting experimental data, it is usually assumed that additives distribute randomly among the micelles. During the award period experiments will be conducted on some well studied systems, like SDS, LiDS, and CTAC in the presence of additives. In the TRFQ method the real time decay on the pyrene fluorescence is measured. The decay time is shortened (quenched) by the quenchers as a result of collisions with the probe. The quenching rate constant depends on the collision frequency between probe and quencher in or at the micelles, which in turn depends on themicelle size. A framework is available that relates the overall decay characteristics and the quenching rate constant to the average size and size disperson of micelles. In the complementary EPR experiments to be carried out by Prof. Bales at CSUN,on the same systems, spin relaxation time of nitroxide free radicals will be measured. Bimolecular collisions shorten the relatation time and the collision frequency depends on the micelle size. The extracted micelle sizes and distribution rely on assumptions of the statistical distribution of all additives on the micelles. The statistical distribution of the additives will be adjusted to conform to data from both experiments. The two techniques used together on the same system will thus give an accurate and reliable determination of the sizes of micelles and of the distribution of the additives.