CYTOKINE RESPONSES OF INJURED ENDOTHELIUM

在成功的伤口愈合事件中，内皮细胞 邻近机械伤口部位的人必须意识到， 发生;迁移到该地区;扩散，以恢复有缺陷的地区; 最后恢复到非增殖状态。 这个过程涉及 许多基因的调控。 为了阐明这些基因， 使用内皮细胞培养物的愈合模型，维持在人工 IV型胶原的基底膜，正在开发中。 微分 展示将用于分离和鉴定受调控的mRNA， 在伤口愈合过程中。 这种方法，沿着使用 感染性心内膜炎的体内模型，可以提供对 损伤或患病心脏愈合的潜在疾病机制 瓣膜可能成为血小板发育的部位 植被 创伤愈合过程中的差异基因调控， 无血管的胶原瓣膜组织可能在决定 无论是修复还是植被形成。

In a successful wound healing event, endothelial cells adjacent to a site of mechanical wound must perceive that an injury has occurred; migrate into the region; proliferate to restore the flawed area; and finally, return to a nonproliferative state. This process involves the regulation of many genes. To elucidate these genes, an in vitro wound healing model using endothelial cell cultures, maintained on an artificial basement membrane of type IV collagen, is being developed. Differential display will be utilized to isolate and identify mRNAs that are regulated during this wound healing process. This approach, along with the use of an in vivo model of infective endocarditis, may provide insights into the disease mechanisms underlying the healing of injured or diseased heart valves which may become the site for the development of platelet vegetations. Differential gene regulation during wound healing of this relatively avascular, collagenous valve tissue, may play a key role determining whether repair or vegetation formation occurs.