WOUND HEALING MODEL USING ENDOTHELIAL CELL CULTURES

在成功的伤口愈合事件中，邻近的内皮细胞 必须意识到，损伤已经 发生;迁移到该地区;扩散，以恢复 有缺陷的区域;最后，恢复到非增殖状态。 这个过程涉及到许多基因的调控。 阐明 这些基因，使用内皮细胞的体外伤口愈合模型， 细胞培养，维持在人工基底膜上， IV型胶原蛋白正在开发中。 差分显示将 用于分离和鉴定受调控的mRNA， 在伤口愈合过程中。 这种方法，沿着 使用感染性心内膜炎的体内模型，可以提供 深入了解疾病的治疗机制， 受伤或患病的心脏瓣膜。 它可能会成为 血小板赘生物的发展。 差异基因 在伤口愈合过程中， 胶原瓣膜组织，可能在决定是否 修复或植被形成。 关键词：创伤愈合，内皮细胞，分化 显示感染性心内膜炎

In a successful wound healing event, endothelial cells adjacent to a site of mechanical wound must perceive that an injury has occurred; migrate into the region; proliferate to restore the flawed area; and finally, return to a nonproliferative state. This process involves the regulation of many genes. To elucidate these genes, an in vitro wound healing model using endothelial cell cultures, maintained on an artificial basement membrane of type IV collagen, is being developed. Differential display will be utilized to isolate and identify mRNAs that are regulated during this wound healing process. This approach, along with the use of an in vivo model of infective endocarditis, may provide insights into the disease mechanisms underlying the healing of injured or diseased heart valves. Which may become the site for the development of platelet vegetations. Differential gene regulation during wound healing of this relatively avascular, collagenous valve tissue, may play a key role determining whether repair or vegetation formation occurs. Key words: wound healing, endothelial cell, differential display, infective endocarditis.