X-CHROMOSOME INACTIVATION AND DNA METHYLATION

X 染色体失活和 DNA 甲基化

• 批准号: 3119678
• 负责人: ARTHUR D RIGGS
• 金额: $ 27.49万
• 依托单位: BECKMAN RESEARCH INSTITUTE/CITY OF HOPE
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3119678
• 关键词: 5 methylcytosine; DNA binding protein; DNA methylation; cell differentiation; cytosine; gene expression; genetic promoter element; genetic transcription; human population genetics; laboratory mouse; methyltransferase; nucleic acid chemical synthesis; oligonucleotides; protein biosynthesis; tissue /cell culture; transposon /insertion element

This grant will support research designed to help answer the following questions: (1) What is the molecular mechanism of mammalian X-chromosome inactivation? (2) What is the biological function(s) of 5-methylcytosine in mammalian DNA? The working hypotheses that will guide this work are: (1) 5- Methylcytosine plays a significant role in mammalian gene regulation and cell differentiation, and (2) 5-Methylcytosine is an important component of the X-chromosome inactivation system. The specific aims are to: (1) identify and study proteins that bind specifically, in vitro and in vivo, to X-linked promoters and X-chromosomal DNA, (2) prepare and study DNA methylated at cytosine residues in CpG doublets by human DNA methyltransferase, (3) study proteins that bind preferentially to enzymatically methylated DNA, and (4) determine the effect of methylation on proteins identified as a result of aim 1.

这笔赠款将支持旨在帮助回答 主要问题有：(1)其分子机制是什么？ 哺乳动物X染色体失活？(2)什么是生物学 5-甲基胞嘧啶在哺乳动物DNA中的作用(S)？ 指导这项工作的工作假设是：(1)5- 甲基胞嘧啶在哺乳动物基因中的重要作用 调节和细胞分化，以及(2)5-甲基胞嘧啶是 X染色体失活系统的重要组成部分。 其具体目标是：(1)鉴定和研究 在体外和体内，特异性地结合X-连锁启动子和 X染色体DNA，(2)胞嘧啶甲基化DNA的制备和研究 人DNA甲基转移酶在CpG双体中的残基(3)研究 优先与酶甲基化的DNA结合的蛋白质， 以及(4)确定甲基化对识别的蛋白质的影响 作为目标1的结果。