X-CHROMOSOME INACTIVATION AND DNA METHYLATION

这笔赠款将支持旨在帮助回答 问题：（1）分子机制是什么 哺乳动物X染色体失活 (2)什么是生物学 哺乳动物DNA中5-甲基胞嘧啶的功能？ 指导这项工作的工作假设是：（1）5- 甲基胞嘧啶在哺乳动物基因组中起重要作用 调节和细胞分化，和（2）5-甲基胞嘧啶是 X染色体失活系统的重要组成部分。 具体目标是：（1）鉴定和研究 在体外和体内特异性结合X-连锁启动子， X染色体DNA，（2）制备并研究胞嘧啶甲基化的DNA 人DNA甲基转移酶法测定CpG双联体中的CpG残基，（3）研究 优先结合酶促甲基化DNA的蛋白质， 和（4）确定甲基化对鉴定的蛋白质的影响 由于目标1。

This grant will support research designed to help answer the following questions: (1) What is the molecular mechanism of mammalian X-chromosome inactivation? (2) What is the biological function(s) of 5-methylcytosine in mammalian DNA? The working hypotheses that will guide this work are: (1) 5- Methylcytosine plays a significant role in mammalian gene regulation and cell differentiation, and (2) 5-Methylcytosine is an important component of the X-chromosome inactivation system. The specific aims are to: (1) identify and study proteins that bind specifically, in vitro and in vivo, to X-linked promoters and X-chromosomal DNA, (2) prepare and study DNA methylated at cytosine residues in CpG doublets by human DNA methyltransferase, (3) study proteins that bind preferentially to enzymatically methylated DNA, and (4) determine the effect of methylation on proteins identified as a result of aim 1.