CHEMOTAXIS AND PERIODONTOPATHOGENS--T DENTICOLA

趋化性和牙周病原--牙周病菌

• 批准号: 3425264
• 负责人: JOHN A MAYO
• 金额: $ 2.09万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3425264
• 关键词: Treponema; aminoacid; anaerobic bacteria; apical membrane; arginine; carbohydrates; cell motility; chemotaxis; fatty acids; gingival sulcus; glucose; microorganism culture; microorganism genetics; microorganism metabolism; oxidation reduction reaction; periodontium disorder; tooth loss; vitamins

Periodontal disease is the major cause of tooth loss in adults. This disease is caused by bacteria, and one of the major goals of recent oral microbiology research has been the identification of the causative agents and the elucidation of their pathogenic mechanisms. Several candidate bacterial groups have been identified, and oral spirochetes presently are of great interest. Oral spirochetes are anaerobic, motile bacteria inhabiting the gingival crevice, where they are found in small numbers. In periodontal disease, these bacteria proliferate greatly and can account for as much as 50 percent of the microbial numbers in diseased sites. They are found at the advancing fronts of lesions, and have been associated with the most destructive forms of periodontal disease in which bone supporting the teeth is undergoing active destruction. The reasons for this distribution in diseased sites are not clear. Motile bacteria are chemotactic; i.e., they respond to chemical signals and move towards nutrients and away from irritants. Bacterial motility and chemotaxis have been invoked to explain the distribution of spirochetes in periodontal lesions, but no specific hypotheses have been tested. In this proposal it is planned to cultivate the oral spirochete Treponema denticola under strict anaerobiosis, and to determine growth conditions which produce highly motile bacteria. Preparations of motile bacteria will be tested for positive and negative chemotaxis to a variety of physiologically significant chemicals which might be found in periodontal pockets. These will include sugars, amino acids, vitamins, and fatty acids. Also, salts used in some types of periodontal therapy will be tested, as will the environmentally significant parameter, redox potential. These experiments will determine whether an oral spirochete can respond chemotactically under conditions which might be found in periodontal disease. Further experiments will probe the physiological regulation of chemotactic behavior in T. denticola.

牙周病是成年人牙齿脱落的主要原因。 这种疾病是由细菌引起的， 最近的口腔微生物学研究已经鉴定出 病原体及其致病性的阐明 机制等 几个候选细菌群已经被 目前，口腔螺旋体已被鉴定出来，引起了人们的极大兴趣。 口腔螺旋体是厌氧的，活动的细菌， 牙龈缝隙，在那里他们被发现在少数。 在 牙周病，这些细菌大量繁殖， 占到了微生物数量的50%， 患病部位。 它们位于病变的前沿， 与最具破坏性的 牙周病，其中支持牙齿的骨被 正在遭受积极的破坏。 这种分布的原因 发病部位不清楚。 能动细菌具有趋化性; 也就是说，它们会对化学信号做出反应， 远离刺激物 细菌的运动性和趋化性 被用来解释螺旋体的分布， 牙周病变，但没有具体的假设进行了测试。 在这个提议中，计划培养口腔螺旋体 严格厌氧条件下的齿垢密螺旋体，并确定 生长条件，产生高度能动的细菌。 将对运动细菌制剂进行阳性和 对多种生理学上显著的 牙周袋中可能存在的化学物质。 这些 将包括糖、氨基酸、维生素和脂肪酸。 还有， 将测试用于某些类型牙周治疗的盐， 将是环境重要参数，氧化还原电位。 这些实验将确定口腔螺旋体是否能 化学反应的条件下，可能会发现， 牙周病 进一步的实验将探索 T.趋化行为的生理调节。齿垢