FLOW CYTROMETRIC ANALYSIS OF HIV-INFECTED PBMC

一种新的、相对便宜且准确的流式细胞术技术， 其识别和定量外周血单核细胞的数量， 感染人类免疫缺陷病毒（HIV）的PBMC，将被 用于监测抗病毒治疗如齐多夫定（AZT）的效果， 双脱氧胞苷（ddC）和双脱氧肌苷（ddi）对HIV-1数量的影响。 在接受这些治疗的艾滋病患者中感染的细胞。 此外，本发明还提供了一种方法， 这些抗病毒化合物对细胞中游离病毒量的影响 将使用p24抗原捕获酶联免疫吸附测定法测量循环。 免疫吸附测定（ELISA）。 我们希望能够测量到 使用流式细胞仪检测单个核细胞中的HIV含量 技术和血浆中游离病毒量的减少， p24抗原捕获ELISA技术。 这些研究比较了 这两种技术测量病毒量的能力 接受化疗的艾滋病患者的血液循环 应该导致有用的和有意义的检测，用于监测的影响， 抗病毒治疗对细胞相关和无细胞HIV数量的影响 这些病人。 血液循环中艾滋病毒数量的任何减少都会 与患者临床状态的改善相关， 这些HIV感染者的其他改善指标。

A novel, relatively inexpensive and accurate flow cytometric technique, which identifies and quantitates the number of peripheral blood mononuclear cells (PBMC) infected with the human immunodeficiency virus (HIV), will be used to monitor the effect of antiviral therapies such as Zidovudine (AZT), dideoxycytidine (ddC), and dideoxyinosine (ddi) on the number of HIV- infected cells in AIDS patients undergoing these treatments. In addition, the effects of these antiviral compounds on the amount of free virus in the circulation will be measured using the p24 antigen-capture enzyme-linked immunosorbant assay (ELISA). We expect to be able to measure a decrease in the amount of HIV in the mononuclear cells using the flow cytometric technique and a decrease in the amount of free virus in the plasma using the p24 antigen-capture ELISA technique. These studies comparing the ability of these two techniques to measure the amount of virus in the circulation of AIDS patients undergoing chemotherapy for their disease should lead to useful and meaningful assays for monitoring the effect of antiviral therapies on the amount of cell-associated and cell-free HIV in these patients. Any decreases in the amount of HIV in the circulation will be correlated with the improvement in clinical status of the patient and other markers of improvement in these HIV-infected patients.