FLOW CYTROMETRIC ANALYSIS OF HIV-INFECTED PBMC

HIV 感染的 PBMC 的流式细胞术分析

• 批准号: 3422605
• 负责人: James McSharry
• 金额: $ 7.5万
• 依托单位: ALBANY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-30 至 1991-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3422605
• 关键词: AIDS; AIDS /HIV diagnosis; AIDS therapy; HIV infections; antiAIDS agent; deoxycytidine; enzyme linked immunosorbent assay; flow cytometry; human immunodeficiency virus 1; human subject; human therapy evaluation; inosine; monocyte; zidovudine

A novel, relatively inexpensive and accurate flow cytometric technique, which identifies and quantitates the number of peripheral blood mononuclear cells (PBMC) infected with the human immunodeficiency virus (HIV), will be used to monitor the effect of antiviral therapies such as Zidovudine (AZT), dideoxycytidine (ddC), and dideoxyinosine (ddi) on the number of HIV- infected cells in AIDS patients undergoing these treatments. In addition, the effects of these antiviral compounds on the amount of free virus in the circulation will be measured using the p24 antigen-capture enzyme-linked immunosorbant assay (ELISA). We expect to be able to measure a decrease in the amount of HIV in the mononuclear cells using the flow cytometric technique and a decrease in the amount of free virus in the plasma using the p24 antigen-capture ELISA technique. These studies comparing the ability of these two techniques to measure the amount of virus in the circulation of AIDS patients undergoing chemotherapy for their disease should lead to useful and meaningful assays for monitoring the effect of antiviral therapies on the amount of cell-associated and cell-free HIV in these patients. Any decreases in the amount of HIV in the circulation will be correlated with the improvement in clinical status of the patient and other markers of improvement in these HIV-infected patients.

一种新的、相对便宜和准确的流式细胞仪技术， 它识别和定量外周血单核细胞的数量 感染人类免疫缺陷病毒(HIV)的细胞(PBMC)将被 用于监测齐多夫定(AZT)等抗病毒治疗的效果， 二脱氧胞苷(DdC)和双脱氧肌苷(Ddi)对HIV- 接受这些治疗的艾滋病患者体内的感染细胞。此外, 这些抗病毒化合物对人体内游离病毒量的影响 将使用p24抗原捕获酶连接来测量循环。 免疫吸附试验(EL ISA)。我们预计将能够测量到 用流式细胞术检测单个核细胞中的HIV含量 技术和血浆中游离病毒量的降低 P24抗原捕获酶联免疫吸附试验技术。这些研究比较了 这两种技术测量人体内病毒数量的能力 艾滋病患者化疗后的循环情况 应导致有用和有意义的分析，以监测的效果 抗病毒治疗对人体内细胞相关和非细胞HIV含量的影响 这些病人。循环中艾滋病毒数量的任何减少都将 与患者临床状况的改善和 这些艾滋病毒感染患者病情改善的其他标志。

项目成果

Oral combination chemotherapy in the management of AIDS-related lymphoproliferative malignancies.

口服联合化疗治疗艾滋病相关淋巴增殖性恶性肿瘤。

• DOI: 10.2165/00003495-199958003-00014
• 发表时间: 1999
• 期刊: Drugs
• 影响因子: 11.5
• 作者: Remick,SC;Sedransk,N;Haase,R;Craffey,M;Subramanian,N;Dowlati,A;Nazeer,T;Ramnes,C;Blanchard,C;Mastrianni,D;Balducci,L;Horton,J;Ruckdeschel,JC
• 通讯作者: Ruckdeschel,JC