FLOW CYTOMETRIC ANALYSIS OF PBMC AFTER HIV-CHEMOTHERAPY

HIV 化疗后 PBMC 的流式细胞术分析

• 批准号: 3147393
• 负责人: James McSharry
• 金额: $ 23.68万
• 依托单位: ALBANY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3147393
• 关键词: 2'3' dideoxycytidine; 2'3' dideoxyinosine; AIDS; AIDS therapy; cytomegalovirus; flow cytometry; human immunodeficiency virus; human subject; human therapy evaluation; immunofluorescence technique; immunologic techniques; macrophage; monocyte; polymerase chain reaction; zidovudine

The long term goal of this project is to develop further a novel technique involving immunofluorescence in conjunction with flow cytometry. This technique will be validated by comparing data from the flow cytometric technique with that derived from the quantitative polymerase chain reaction (PCR) and infectivity assays. Validation of this novel flow cytometric technique will provide a relatively inexpensive and rapid method for detecting and quantitating the number of HIV-infected peripheral blood mononuclear cells (PBMC) in HIV- seropositive individuals. This technique will be used to monitor disease progression in HIV-infected individuals and monitor the effects of anti- retroviral chemotherapies on virus burden in these individuals. The specific aims of this grant proposal are: 1. Validate the flow cytometric technique by comparing data obtained from flow cytometry with that derived from PCR and infectivity assays. 2. Use the flow cytometric technique to monitor patients who are receiving anti-retroviral chemotherapy such as ZDV, ddc and ddi. 3. To use the flow cytometric technique to identify the cells in the peripheral blood that are infected with HIV. 4. To use the flow cytometric to identify the cells in the peripheral blood that are infected with cytomegalovirus (CMV). Detection and quantitation of the PBMC infected with CMV may help elucidate the role that this opportunistic infection plays in the pathogenesis of AIDS. Successful completion of these studies will validate a technique that will make monitoring of the number of HIV-infected PBMC rapid and relatively inexpensive.

这个项目的长期目标是进一步开发一种新的 涉及免疫荧光与流动相结合的技术 细胞仪这项技术将通过比较来自 流式细胞术技术与来自定量 聚合酶链反应（PCR）和感染性测定。验证 这种新的流式细胞术技术将提供一种相对 廉价和快速的方法来检测和定量的数量 HIV感染的外周血单核细胞（PBMC）在HIV- 血清阳性个体。这项技术将用于监测疾病 艾滋病毒感染者的进展，并监测抗 逆转录病毒化疗对这些个体的病毒负荷的影响。 这项赠款建议的具体目标是： 1.通过比较获得的数据来验证流式细胞术 与来自PCR和感染性测定的结果进行比较。 2.使用流式细胞仪技术监测以下患者 接受抗逆转录病毒化疗，如ZDV、DDC和DDI。 3.应用流式细胞仪技术鉴定肿瘤组织中的细胞 感染艾滋病病毒的外周血。 4.使用流式细胞仪鉴定外周血细胞 感染巨细胞病毒（CMV）的血液。检测和 对感染CMV的PBMC进行定量分析可能有助于阐明CMV在PBMC中的作用。 这种机会性感染在艾滋病发病机制中的作用。 这些研究的成功完成将验证一种技术， 将快速监测HIV感染PBMC的数量， 相对便宜。