VIDEO IMAGING AND NEONATAL BRAIN OXYGENATION

在新生儿中，保持大脑的良好氧合是非常重要的 以避免不可逆的功能丧失。 我们开发了新 成像技术，以获得高分辨率（< 5 μ m/像素），定量， 二维地图氧气压力的静脉和毛细血管床的 皮质，以及细胞内pH和细胞内Ca 2 + 大脑皮层细胞表层的浓度。 这些 在新生仔猪中测量的代谢物图谱将使我们能够定量 皮层的氧合和细胞生理学对 PaCO 2、PaO 2、血液pH和全身血压。 “临界”氧 皮质毛细血管床中的压力，即低于 在EEG、细胞内pH或细胞内Ca 2+中观察到改变 浓度将被确定。 每种疾病的严重程度和持续时间 缺氧/代谢紊乱需要引起不可逆损伤， 由测量参数未能恢复正常值指示， 也将被确定。 具体来说，我们将：A。进行定量 皮质氧分压和功能对PaCO 2的依赖性。 B。 定量向皮质输送氧气对 全身血压 C.定量PaCO 2和PaO 2的相互作用 调节皮质局部微血管系统的作用 D.确定 代谢性酸血症对皮质氧合和功能的影响。 E. 评价微血管损伤在不可逆脑损伤中的作用 功能 将开展合作研究，以将上述内容联系起来 通过微透析评价神经递质代谢参数。 这些措施将允许关键的有效性评价， 旨在优化氧气输送并减少不可逆 对新生儿的大脑造成伤害。

In the newborn it is extremely important to keep the brain well oxygenated in order to avoid irreversible loss of function. We have developed new imaging technology to obtain high resolution (< 5 um/pixel), quantitative, two dimensional maps oxygen pressure in the veins and capillary beds of the cortex, as well as of intracellular pH and intracellular Ca2+ concentrations in the surface layers of cell of the brain cortex. These metabolite maps, measured in newborn piglets, will allow us to quantitate the dependence of oxygenation and cellular physiology of the cortex on PaCO2, PaO2, blood pH and systemic blood pressure. the "critical" oxygen pressures in the capillary beds of the cortex i.e. those below which alterations are observed in EEG, intracellular pH or intracellular Ca2+ concentration, will be determined. The severity and duration of each hypoxia/metabolic perturbation require to cause irreversible damage, as indicated by failure of the measured parameters to recover normal values, will also be determined. Specifically we will: A. Quantitate the dependence of cortical oxygen pressure and function on PaCO2. B. Quantitate the dependence of delivery of oxygen to the cortex effect on systemic blood pressure. C. Quantitate the interactions of PaCO2 and PaO2 in regulating the local microvascular system of the cortex. D. Determine the effect of metabolic acidemia on cortical oxygenation and function. E. Evaluate the role of microvascular damage in irreversible damage to brain function. Collaborative studies will be carried out to correlate the above parameters with neurotransmitter metabolism as evaluated by microdialysis. These measures will permit critical evaluation of the efficacy of treatments designed to optimize oxygen delivery and to reduce irreversible damage to the brain of the newborn.