OXYGEN MEASUREMENTS IN VIVO BY PHOSPHORESCENCE QUENCHING

通过磷光淬火进行体内氧气测量

• 批准号: 6184402
• 负责人: DAVID F WILSON
• 金额: $ 21.57万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-06 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6184402
• 关键词: analytical method; chemical synthesis; clinical biomedical equipment; computational neuroscience; diagnosis design /evaluation; mathematical model; method development; noninvasive diagnosis; optics; oxygen tension; phantom model; phosphorescence; phosphorus compounds; photochemistry; respiratory gas analyzer; respiratory oxygenation; vascular endothelium permeability

DESCRIPTION: The investigators propose a program to develop reliable methods based on phosphorescence quenching for the measurement of oxygen concentrations in living animals and superficial tissue volumes in human patients, as further described by their abstract: "Oxygen dependent quenching of phosphorescence is a non invasive, highly accurate, optical method for measuring oxygen, including under conditions for which there are either no other suitable method or the existing methods are inadequate. This is new technology which is badly needed in biomedical research and as a diagnostic tool in the clinics, because it can be used non invasive, real time, measurements of tissue oxygenation under physiological conditions and in real time. In order to bring this new technology to its full potential and to make it accessible to a maximum number of users, we will: 1. Design and synthesize water soluble, non-toxic phosphors which absorb and phosphoresce in the near infra red 'window' of tissue. A. Determine the role of the phosphor microenvironment on the oxygen quenching process, e.g. access of oxygen to the phosphor, dependence on pH and temperature, photochemical stability, etc.; B. Determine the chemical properties which control permeability of the phosphors through the vascular wall (leakage into the intravascular space) and of excretion by the kidney. 2. Develop new, high performance, frequency domain phosphorometers for measuring oxygen pressure in vivo. 3. Develop the mathematical formalism needed for extraction of oxygen distributions (histograms) for the microvasculature by computational analysis of the measured phosphorescence decays. 4. Construct and test new optical systems capable of providing 3D maps, at millimeter resolution, of regions of relative hypoxia in tissue volumes several centimeters on a side. "Potential clinical applications include diagnosis and clinical evaluation of complications of diabetes, peripheral vascular disease, cerebrovascular and cardiovascular events as well as detection and therapy design for treatment of tumors."

描述：研究人员提出了一项计划，以开发可靠的 基于磷光猝灭的氧测量方法 活体动物和人体浅表组织中的浓度 患者，如他们的摘要进一步描述： “磷光的氧依赖性猝灭是一种非侵入性的，高度 测量氧气的精确的光学方法，包括在 没有其他合适的方法或现有的方法 是不够的。 这是生物医学领域急需的新技术 研究和作为诊断工具，在诊所，因为它可以使用非 生理条件下组织氧合的侵入性真实的实时测量 条件和真实的时间。 为了将这项新技术带到 充分发挥其潜力，并使其可供最大数量的用户使用，我们 意志：1. 设计和合成水溶性无毒磷光体， 在组织的近红外“窗口”吸收和发磷光。 A. 确定磷光体微环境对氧猝灭的作用 过程，例如氧进入磷光体，对pH的依赖性， 温度、光化学稳定性等; B。确定化学 控制磷光体通过血管的渗透性的性质 壁（渗漏到血管内空间）和肾脏排泄。 2. 开发新的高性能频域磷光计， 测量体内氧分压。 3. 发展数学形式主义 所需的氧分布（直方图）的提取 通过测量磷光的计算分析微脉管系统 腐烂 4. 构建和测试能够提供3D的新光学系统 组织中相对缺氧区域的毫米分辨率地图 每边几厘米的体积。 “潜在的临床应用包括诊断和临床评估 糖尿病并发症、周围血管疾病、脑血管疾病 和心血管事件以及检测和治疗设计 肿瘤的治疗。"