MEASUREMENT OF ALKENYL/EPOXY DNA ADDUCTS BY GC-MS

将开发新的分析方法来测量DNA加合物 因人类职业暴露于乙烯、环氧乙烷、 丁二烯、苯乙烯和丙烯。这些化学物质是从 它们被广泛使用，并具有遗传毒性。此外，他们还预计会给出 相同类型的DNA加合物。 该方法主要包括四个步骤：(1)分离 从生物样本中提取DNA；(2)将DNA加合物从 大量DNA；(3)电泳液衍生加合物；以及(4)检测 气相色谱-电子捕获法测定电泳产物 负离子质谱仪(GC-ECNI-MS)。电选 选择衍生化/GC-ECNI-MS是因为需要 灵敏、明确的测量方法。目前的方法论仅限于 这些方面，这使得很难对 暴露和风险。 测量人体样本中的DNA加合物是很重要的。DNA是一种 体内致癌和致突变化学物质的最终靶标。 DNA加合物的测量可以帮助解决不确定因素 从个人接触多种化学品和从个人 新陈代谢和生活方式的变化。我们对这一现象的理解 化学损伤对DNA的生物学意义取决于我们的 衡量它的能力。

New analytical methodology is to be developed to measure DNA adducts arising from occupational human exposure to ethylene, ethylene oxide, butadiene, styrene and propene. These chemicals were selected since they are widely used and genotoxic. Also they are anticipated to give the same types of DNA adducts. The proposed methodology basically comprises four steps: (1) isolate the DNA from a biological sample; (2) separate the DNA adducts from the bulk of the DNA; (3) electrophore derivatize the adducts; and (4) detect the electrophoric products by gas chromatography - electron capture negative ion-mass spectrometry (GC-ECNI-MS). Electrophoric derivatization/GC-ECNI-MS has been selected because of the need for sensitive, definitive measurements. Current methodology is limited in these respects, which makes it difficult to make accurate assessments of exposure and risk. It is important to measure DNA adducts in human samples. DNA is an ultimate target in the body for carcinogenic and mutagenic chemicals. Measurements of DNA adducts can help to sort out uncertainties arising from exposure of individuals to multiple chemicals, and from individual variations in metabolism and lifestyle. Our understanding of the biological significance of chemical damage to DNA is dependent on our ability to measure it.