Radiation Biology of EPR Oxygen Images

EPR 氧气图像的放射生物学

• 批准号: 7582532
• 负责人: HOWARD J HALPERN
• 金额: $ 27.33万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7582532
• 关键词: Animal Model; Animals; Breast Carcinoma; Clinical; Dose; Effectiveness; Electron Spin Resonance Spectroscopy; Electrons; Funding; Histologic; Human; Hypoxia; Hypoxia Inducible Factor; Image; Inbred C3H Mice; Injection of therapeutic agent; Investigation; Leg; Magnetic Resonance; Magnetic Resonance Imaging; Malignant Neoplasms; Measurement; Mus; Oryctolagus cuniculus; Outcome; Oxygen; Pimonidazole; Poison; Population; Proteins; Radiation; Radiobiology; Rattus; Reporting; Resistance; Resolution; Staining method; Stains; Subgroup; Techniques; Testing; Time; Tumor Oxygenation; Vascular Endothelial Growth Factors; Width; Work; base; carbonate dehydratase; fibrosarcoma; glucose uptake; improved; melanoma; mouse model; receptor; statistics; tumor

Electron Paramagnetic Resonance (EPR Oxygen Images (EPROI) have been shown to provide quantitative localized PO2 images of animal tumors including several in syngeneic mice, and in rats, and rabbits. The oxygen broadening of narrow EPR spectral lines report the PO2 with 1-3 torr resolution in each 1mm3 image voxeL This work has shown i) the first co-localized correlation between an oxygen image and a point oxygen measurement technique (Oxylite) 2) independent, statistically significant sharpening of the 50% tumor control dose with the addition of the information provided by the fraction of EPROI voxels less than io torr (HFio) 3) co-registration of pimonidazole images nth EPROI PO2 and 4) significant spatial correlation of VEOF and EPROI based HEw. We propose the following specific aims for the given cycle: i) Determining the relation between the localized oxygen information from the EPROI and pimonidazole retention and induction of hypoxia proteins, 2) Treatment of murine tumors to TCDSO after oxygen images and determining the predictive effectiveness of the HFio and other EPROT tumor statistics.

电子顺磁共振（EPR）氧图像（EPROI）已被证明可以提供动物肿瘤的定量定位PO 2图像，包括同系小鼠、大鼠和兔中的几种肿瘤。窄EPR谱线的氧增宽在每个1 mm ~ 3图像体素中以1-3 μ m的分辨率报告了PO_2。这项工作表明：i）氧图像和点氧测量技术（Oxylite）之间的第一个共定位相关性; 2）独立，统计学上显著锐化的50%肿瘤控制剂量，加上由EPROI体素的分数小于100（HFio）提供的信息，3）哌莫硝唑图像的共配准，第n个EPROI PO 2和4）基于HEW的VEOF和EPROI的显著空间相关性。我们针对给定周期提出了以下具体目标：i）确定来自EPROI的局部氧信息与哌莫硝唑保留和缺氧蛋白诱导之间的关系，2）在氧图像后用TCDSO治疗小鼠肿瘤并确定HFio和其他EPROT肿瘤统计的预测有效性。