Radiation Biology of EPR Oxygen Images

EPR 氧气图像的放射生物学

• 批准号: 7862342
• 负责人: HOWARD J HALPERN
• 金额: $ 27.93万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7862342
• 关键词: Animal Model; Animals; Biological Assay; Biopsy; Breast Carcinoma; Carcinoma; Clinical; Dose; ENG gene; Effectiveness; Electron Spin Resonance Spectroscopy; Electrons; Funding; Histologic; Human; Hypoxia; Hypoxia Inducible Factor; Image; Imaging Techniques; Inbred C3H Mice; Injection of therapeutic agent; Intensity-Modulated Radiotherapy; Investigation; Magnetic Resonance; Magnetic Resonance Imaging; Malignant Neoplasms; Measurement; Mus; Oryctolagus cuniculus; Outcome; Oxygen; Pimonidazole; Proteins; Radiation; Radiobiology; Rattus; Relative (related person); Reporting; Resistance; Resolution; Subgroup; Techniques; Testing; Tumor Oxygenation; Tumor Volume; Vascular Endothelial Growth Factors; Work; base; fibrosarcoma; glucose uptake; improved; melanoma; mouse model; receptor; small molecule; statistics; tumor

Electron Paramagnetic Resonance (EPR Oxygen Images (EPROI) have been shown to provide quantitative localized PO2 images of animal tumors including several in syngeneic mice, and in rats, and rabbits. The oxygen broadening of narrow EPR spectral lines report the PO2 with 1-3 torr resolution in each 1mm3 image voxeL This work has shown i) the first co-localized correlation between an oxygen image and a point oxygen measurement technique (Oxylite) 2) independent, statistically significant sharpening of the 50% tumor control dose with the addition of the information provided by the fraction of EPROI voxels less than io torr (HFio) 3) co-registration of pimonidazole images nth EPROI PO2 and 4) significant spatial correlation of VEOF and EPROI based HEw. We propose the following specific aims for the given cycle: i) Determining the relation between the localized oxygen information from the EPROI and pimonidazole retention and induction of hypoxia proteins, 2) Treatment of murine tumors to TCDSO after oxygen images and determining the predictive effectiveness of the HFio and other EPROT tumor statistics.

电子顺磁共振(EPR O2 Images，EPROI)已被证明可以提供动物肿瘤的定量定位PO2图像，包括几个同基因小鼠、大鼠和兔的肿瘤。狭窄EPR谱线的氧气展宽报告了在每个1mm3图像体素中具有1-3Torr分辨率的PO2这项工作已经表明：i)氧图像和点氧测量技术(Oxylite)之间的第一个共局域相关性2)独立的、统计上显著的锐化50%的肿瘤控制剂量与添加小于io torr(HFio)的部分EPROI体素提供的信息3)与EPROI PO2图像的联合配准以及4)基于VEOF和EPROI的HEW的显著空间相关性。对于给定的周期，我们提出了以下具体目标：1)确定来自EPROI的局部氧信息与匹莫硝唑保留和诱导低氧蛋白之间的关系；2)在氧成像后对小鼠肿瘤进行TCDSO治疗，并确定HFIO和其他EPROT肿瘤统计数据的预测效果。