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Molecular Biology Core

分子生物学核心

基本信息

批准号：
10618376
负责人：
Jon Scott Blevins
金额：
$ 22.8万
依托单位：
UNIV OF ARKANSAS FOR MED SCIS
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-05-05 至 2027-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10618376
关键词：
Address Antibody Formation Applications Grants Arkansas Award Centers of Research Excellence Cloning Core Facility DNA DNA Sequencing Facility DNA biosynthesis DNA sequencing Data Dedications Doctor of Philosophy Ensure Equation Equipment Fee-for-Service Plans Fees Flow Cytometry Funding Gel Genes Genomic Library Genomics Goals Growth Human Resources Immunology Inflammatory Inflammatory Response Institution Investments Laboratory Personnel Maintenance Manuscripts Medical Medicine Methods Microbial Genome Sequencing Microbiology Modernization Molecular Biology Opportunistic Infections Pathogenesis Phase Preparation Proteins RNA Recovery Research Research Infrastructure Research Personnel Resources Sampling Science Scientist Service delivery model Services Side Supervision Technology Time Training Universities Validation Western Blotting Work base cellular imaging college cost equipment acquisition genome-wide instrument instrumentation microbial microscopic imaging next generation next generation sequencing operation pathogen programs protein expression protein purification quantitative imaging response success tool user-friendly voucher

项目摘要

PROJECT SUMMARY/ABSTRACT In Phases I and II, the Center for Microbial Pathogenesis and Host Inflammatory Responses (CMPHIR) established and maintained a Research and Technical Advancement Core to fill experimental gaps in the resources available in institutional cores maintained by the UAMS College of Medicine. One of these was a DNA Sequencing Core. However, the capability of this core was limited to single-gene sequencing. While this was an important and necessary service, it was not sufficient, particularly in an era of genomic and next-generation sequencing technology. Thus, the CMPHIR also invested heavily in augmenting the resources available in the existing DNA Sequencing Core. We accomplished this task, and, in Phase III, we propose to integrate these services under the common umbrella of a Molecular Biology Core (Core B) capable of providing CMPHIR and other UAMS investigators with the resources they need, particularly with respect to the pathogen side of the host–pathogen equation. The activities of Core B include enhancing the capability of obtaining high-quality RNA, DNA, and protein samples for analysis; high-throughput single-gene sequencing using conventional Sanger methods; next-generation sequencing of microbial genomes and genomic libraries; protein expression and purification; and antibody production. The proposed Core B will be supported through a combination of CMPHIR funding and continued institutional support. Daily operations will be supervised by Dr. Jon S. Blevins, Director, who will work with 2 full-time technicians. One of these technicians will have primary responsibility for the DNA component of Core B, while the other will have primary responsibility for the protein component. To ensure consistency and promote self-sustainability, Core B instruments will be operated by these technicians on behalf of CMPHIR and UAMS investigators on a fee-for-service basis. This will allow us to recover a significant proportion of the costs and thereby ensure the ability to upgrade and maintain the equipment in this core as needed as we move forward into Phase III and beyond.

项目总结/文摘