Flow Cytometry Core

项目总结/摘要 在第一阶段和第二阶段，微生物发病机制和宿主炎症反应中心（CMPHIR） 建立并维持了一个研究和技术进步核心，以填补实验空白， 由UAMS医学院维护的机构核心中可用的资源。其中之一是流动 细胞计数核心，其总体重点是允许UAMS研究人员表征细胞群 在各种病理条件下。然而，该流式细胞术核心不足以满足 需要CMPHIR研究人员，因此CMPHIR在现有流式细胞术上投入了大量资金 核心，为这些研究人员提供他们所需的资源，以表征宿主对感染的反应 在细胞水平上，并且在符合BSL-2的条件下这样做。我们完成了这项任务，在第三阶段， 我们建议将这些服务整合到更全面的流式细胞仪的共同保护伞下， 核心（核心C）。拟议的核心C将通过CMPHIR供资和持续的 机构支持。日常操作将由Jason S博士监督。Stumhofer，主任，谁将与 2名专职技术人员。这些技术人员将主要负责操作仪器， 核心C代表CMPHIR和其他UAMS研究者，并与Stumhofer博士协商，协助 这些研究人员在实验设计和数据解释。为了促进自我可持续性，大多数核心C 仪器将由这些技术人员按服务收费操作。会有一些例外， 允许用户自己使用设备，从而在下班后可以使用;在这种情况下， 恢复工作将采用收费访问模式。这将使我们能够收回很大一部分 与核心C相关的成本，并确保有能力升级和维护该核心中的设备 在我们进入第三阶段及以后的时候。

PROJECT SUMMARY/ABSTRACT In Phases I and II, the Center for Microbial Pathogenesis and Host Inflammatory Responses (CMPHIR) established and maintained a Research and Technical Advancement Core to fill experimental gaps in the resources available in institutional cores maintained by the UAMS College of Medicine. One of these was a Flow Cytometry Core, the overall focus of which was to allow UAMS investigators to characterize cellular populations in the context of various pathological conditions. However, this Flow Cytometry Core was not sufficient to meet the needs CMPHIR researchers, and for this reason the CMPHIR invested heavily in the existing Flow Cytometry Core to provide these researchers with the resources they needed to characterize the host response to infection at a cellular level, and to do so under BSL-2–compliant conditions. We accomplished this task, and in Phase III we propose to integrate these services under the common umbrella of this more comprehensive Flow Cytometry Core (Core C). The proposed Core C will be supported through a combination of CMPHIR funding and continued institutional support. Daily operations will be supervised by Dr. Jason S. Stumhofer, Director, who will work with 2 full-time technicians. These technicians will have the primary responsibility for operating instrumentation within Core C on behalf of CMPHIR and other UAMS investigators and, in consultation with Dr. Stumhofer, assisting these investigators in experimental design and data interpretation. To promote self-sustainability, most Core C instruments will be operated by these technicians on a fee-for-service basis. There will be some exceptions to allow the users themselves to utilize the equipment, thus making it accessible after hours; in these cases, cost recovery will be accomplished using a fee-for-access model. This will allow us to recover a significant proportion of the costs associated with Core C and ensure the ability to upgrade and maintain the equipment in this core as needed as we move forward into Phase III and beyond.