Flow Cytometry Core

项目摘要/摘要 在第一和第二阶段，微生物致病和宿主炎症反应中心(CMPHIR) 建立和维护研究和技术进步核心，以填补 由UAMS医学院维持的机构核心中可用的资源。其中之一就是流动 细胞学核心，其总体重点是允许UAMS调查人员表征细胞群体 在各种病理情况下。然而，这一流式细胞术核心不足以满足 需要CMPHIR研究人员，为此，CMPHIR在现有的流式细胞仪上投入了大量资金 核心，为这些研究人员提供他们需要的资源，以表征宿主对感染的反应 在细胞水平上，并且在符合BSL-2的条件下这样做。我们完成了这项任务，在第三阶段 我们建议将这些服务整合到这个更全面的流式细胞术的共同保护伞下 核心(核心C)。拟议的核心C将通过CMPHIR供资和继续供资相结合的方式提供支持 制度保障。日常运营将由主任Jason S.Stumhofer博士监督，他将与 2名专职技术人员。这些技术人员将主要负责在 核心C代表CMPHIR和其他UAMS调查人员，并与Stumhofer博士协商，协助 这些研究人员在实验设计和数据解释方面。为了促进自我可持续性，MOST核心C 仪器将由这些技术人员按服务收费方式操作。将有一些例外情况 允许用户自己使用设备，从而使其可以在下班后使用；在这些情况下，成本 恢复将使用按访问收费的模式完成。这将使我们能够收回相当大的比例 与核心C相关的成本，并确保能够升级和维护此核心中的设备 在我们进入第三阶段及以后的时候，视需要而定。

PROJECT SUMMARY/ABSTRACT In Phases I and II, the Center for Microbial Pathogenesis and Host Inflammatory Responses (CMPHIR) established and maintained a Research and Technical Advancement Core to fill experimental gaps in the resources available in institutional cores maintained by the UAMS College of Medicine. One of these was a Flow Cytometry Core, the overall focus of which was to allow UAMS investigators to characterize cellular populations in the context of various pathological conditions. However, this Flow Cytometry Core was not sufficient to meet the needs CMPHIR researchers, and for this reason the CMPHIR invested heavily in the existing Flow Cytometry Core to provide these researchers with the resources they needed to characterize the host response to infection at a cellular level, and to do so under BSL-2–compliant conditions. We accomplished this task, and in Phase III we propose to integrate these services under the common umbrella of this more comprehensive Flow Cytometry Core (Core C). The proposed Core C will be supported through a combination of CMPHIR funding and continued institutional support. Daily operations will be supervised by Dr. Jason S. Stumhofer, Director, who will work with 2 full-time technicians. These technicians will have the primary responsibility for operating instrumentation within Core C on behalf of CMPHIR and other UAMS investigators and, in consultation with Dr. Stumhofer, assisting these investigators in experimental design and data interpretation. To promote self-sustainability, most Core C instruments will be operated by these technicians on a fee-for-service basis. There will be some exceptions to allow the users themselves to utilize the equipment, thus making it accessible after hours; in these cases, cost recovery will be accomplished using a fee-for-access model. This will allow us to recover a significant proportion of the costs associated with Core C and ensure the ability to upgrade and maintain the equipment in this core as needed as we move forward into Phase III and beyond.