Cellular functions of Ran GTPase

Ran GTPase 的细胞功能

• 批准号: 7733479
• 负责人: Petr Kalab
• 金额: $ 33.85万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7733479
• 关键词: Address; American; Animal Model; Animals; BRCA1 gene; Binding; Biochemical; Biological; Biological Models; Biology; Biosensor; Cell Nucleus; Cell division; Cell physiology; Cells; Cellular biology; Characteristics; Chemicals; Chromatin; Collaborations; DNA; Decision Making; Development; Disease; Enzymatic Biochemistry; Eukaryota; Eukaryotic Cell; Family member; Fluorescence; Future; Genome; Goals; Guanosine Triphosphate Phosphohydrolases; Housing; Human; Image; Importins; International; Interview; Journals; Laboratories; Life; Malignant Neoplasms; Manuscripts; Mediating; Meiosis; Methods; Microscope; Microscopy; Mind; Mitosis; Mitotic; Mitotic spindle; Molecular; Molecular Structure; Monomeric GTP-Binding Proteins; NCI Center for Cancer Research; NPM1 gene; Nuclear; Nuclear Envelope; Oral; Organelles; Pathway interactions; Plants; Positioning Attribute; Protein Overexpression; Proteins; Proteomics; Publishing; Regulation; Reporting; Research; Research Personnel; Research Project Grants; Role; Running; San Francisco; Signal Transduction; Societies; Somatic Cell; Structure; Students; System; TACC3 gene; Vertebrates; Work; Writing; Xenopus; abstracting; alpha Karyopherins; animal facility; base; cell type; design; egg; high throughput screening; human STK6 protein; inhibitor/antagonist; instrumentation; nucleocytoplasmic transport; nucleophosmin; posters; receptor; reconstitution; research study; small molecule; survivin; symposium; tissue/cell culture; tool

Summary of accomplishments (Since May 2008) 1. The laboratory is now equipped to perform basic biochemical and cell biological work. 2. One postdoctoral student was hired (arrives April 2009), several interviewed but no further decisions made so far. 3. The work on the animal aquatic facility is expected to start with the new FY 09 and is supposed to be finalized in October/November 2008 4. The FLIM upgrade to Zeiss 710 confocal microscope was designed and all its components have been purchased. The assembly of the system started on September 29, 2008. 5. High throughput screen for small molecule inhibitors of importin alpha- importin beta interaction has been successfully validated at the NCGC. 6. A chapter for Methods in Enzymology was completed and is in press and a research manuscript (collaboration) is under review in Biophysical Journal. Another invited methods review chapter as well as a research manuscript are in progress. 7. Conferences: oral presentation at the Cold Spring Harbor Meeting (Dynamic Organization of Nuclear Function, Sept. 17-21, 2008), poster abstract submitted to the Annual Meeting of the American Society for Cell Biology (San Francisco, Dec. 2008). 8. Four new national and international collaborations were established

成绩摘要（自2008年5月以来）该实验室现在配备了 进行基本的生物化学和细胞生物学工作。2.一名博士后被录用 （2009年4月到达），对几个人进行了面谈，但迄今没有作出进一步的决定。3.的工作 动物水生设施预计将于2009财年开始， 2008年10月/11月完成4. FLIM升级到Zeiss 710共聚焦显微镜， 已完成设计，所有部件均已采购。该系统的装配开始于 2008年9月29日5.高通量筛选输入素α-受体的小分子抑制剂 已经在NCGC成功验证了输入蛋白β相互作用。6.一章为 酶学方法已完成，并在印刷和研究手稿（合作） 正在生物物理学杂志上进行评论。另一个邀请的方法审查章节以及一个 研究手稿正在进行中。7.会议：在冷泉会议上的口头报告 港口会议（核功能的动态组织，9月。17-21，2008年），海报摘要 提交给美国细胞生物学学会年会（San弗朗西斯科，12月12日）。 2008年）。8.建立了四个新的国家和国际合作