RAN-REGULATED IMPORTIN BETA CARGOS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 RAN GTP酶是许多细胞过程的关键调节因子，包括细胞核和细胞质之间的运输以及有丝分裂纺锤体的组装。RAN的许多这样的功能是由RanGTP诱导的Importinβ结合蛋白的释放所介导的。我们开发了一种策略，以定量和高纯度的形式分离Ran调节的Importinβ货物。通过泥坑分析鉴定Importinβ“转运体”将有助于发现和理解RAN的间期和有丝分裂功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ran GTPase is a key regulator of many cellular processes including the transport between nucleus and cytoplasm and of the mitotic spindle assembly. Many of such functions of Ran are mediated by RanGTP induced release of importin beta-binding protein cargos. We developed strategy to isolate Ran-regulated importin beta cargos quantitatively and in a highly purified form. The identification of the importin beta "transportome" by MuDPIT analysis will facilitate the discovery and understanding of the interphase and mitotic functions of Ran.