RAN-REGULATED IMPORTIN BETA CARGOS

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 Ran GT3是许多细胞过程的关键调节因子，包括细胞核和细胞质之间的运输以及有丝分裂纺锤体组装。Ran的许多这样的功能是由RanGTP诱导的输入蛋白β结合蛋白货物的释放介导的。我们开发了一种策略，以定量和高度纯化的形式分离RAN调节的输入β货物。通过MuDPIT分析鉴定importin β“转运体”将有助于发现和理解Ran的间期和有丝分裂功能。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ran GTPase is a key regulator of many cellular processes including the transport between nucleus and cytoplasm and of the mitotic spindle assembly. Many of such functions of Ran are mediated by RanGTP induced release of importin beta-binding protein cargos. We developed strategy to isolate Ran-regulated importin beta cargos quantitatively and in a highly purified form. The identification of the importin beta "transportome" by MuDPIT analysis will facilitate the discovery and understanding of the interphase and mitotic functions of Ran.