NHGRI/DIR Animal Research Infrastructure

在OSD下，OLAM支持 NHGRI科学家的研究。这种支持的一个关键重点是在人道主义方面的培训 照顾和处理研究用动物。为此，他们创建了三个培训 已在世界各地发行的CD：1)生存啮齿动物外科培训， NHGRI和OACU的联合项目；2)基础生物方法学培训 实验室老鼠，一个NHGRI资助和生产的项目，也是一个 OACU CD小鼠的脊椎&大鼠的人文束缚与实验 技术；3)对实验大鼠进行基本生物方法学培训 NIH/NHGRI(777号文件)4/08 I-5 目前正在生产的项目，已由AALAS提供部分资金 基金会。通过奥兰提供的培训确保所有动物处理都在 符合NHGRI、NIH和联邦法规和指南的要求 保证获得国际AAALAC的全面认证。奥兰还开发了 和/或建议改进人道关怀和处理，并提供培训 这些改进方面的研究人员。 除了OACU要求的在线或基于讲座的培训和 要注册AEP(参见NIH概述)，NHGRI ACUC需要 在NHGRI ASP上列出或添加到 修改后的ASP。每名研究人员必须： 完成针对设施的培训。本培训包括要求 设施进入，正确的动物处理程序，以及其他相关的 问题。警察或她指定的人进行这项训练。 审查CD的相关章节，《基本培训》 实验小鼠的生物方法学。这张CD是开发出来的 由NHGRI提供财务支持，并涵盖最常见的 在NHGRI进行的程序。这包括一般畜牧业， 约束和处理，鉴定方法，基因分型，注射， 采血、麻醉和止痛，以及安乐死。这个 NHGRI调查员手册还提供了关于 在研究中照顾和使用动物。 一旦审查了裁谈会的相关章节， 与警方或她指定的人进行一对一培训以确保 动物受到人道对待而不会对动物造成伤害或 操作员和技能适合于实验 正在表演的手法(S)。 进行存活啮齿动物手术的个人也被要求 观看CD，培训啮齿动物生存外科开发和 由NHGRI和OACU提供财政支持。这张CD也 介绍转基因手术和基本缝合中的特殊注意事项 技巧。不管调查人员的技能或培训如何，所有的 外科手术由警方观察，直到她确信 所有的无菌操作和外科手术都进行得很好。 如有必要，非恢复期训练动物将被使用，直到 外科手术技巧已经掌握。 签署一份声明，声明已阅读了ASP，其内容为 明白了。 阅读NHGRI ACUC 03.1 LAAPP指南和附加DOHS 过敏宣传册。 培训是一项持续的承诺，也是NHGRI的高度优先事项。这个 奥兰每月在不同的地方举办实践培训课程 实验技术(例如，束缚、采血、注射、 缝合等)NHGRI apd和ACUC主席还提供 关于新的/修订的联邦、NIH和/或NHGRI动物福利的更新 在每月NHGRI教职员工会议上的指导方针。 NHGRI Olam和OACU开发了CD培训 工具，生存啮齿动物外科的培训。这张CD教会了 啮齿动物外科无菌技术的使用原则 转基因手术的考虑，以及简单的缝合方式。 所有NHGRI人员执行任务时都需要查看本CD 啮齿动物生存手术。 术前计划是在制定方案时开始的。 在这段时间里，外科医生的技能和可用的 设施/设备由PI和APD进行评估。如果 调查人员没有经验的外科手术程序，培训 在僵尸或终末麻醉的动物身上 根据NHGRI培训议定书(G-98-1)进行，直到 技术已经完善。一旦议定书获得批准， 警察局与调查人员合作，确保无菌做法 紧随其后。不管调查员的专业水平如何， 当所有手术第一次进行时，警察局都会观察。 此培训记录在OLAM培训文件中并进行维护。 此外，NHGRI apd还提供个性化培训，如 如上文手术前计划部分所述。调查员 与NHGRI警察局的培训几乎每天都在进行。如果 警察局缺乏教授一种特殊技术的专业知识，这是 警察局和私家侦探的共同责任是找到一个 有能力训练警察和调查员。 所有使用麻醉剂的NHGRI调查员都是由警方培训的 在安全和有效地使用它们以及在适当的技术中 诱导、维护和恢复。此培训已记录在案 并保存在奥兰培训文件中。这些技术是 也谈实验小鼠训练的生物方法学 CD.使用吸入性麻醉剂的调查人员还接受了 安全和适当的清理技术。 所有NHGRI调查人员都由动物设施和 警察局或她指定的人在他们的设施培训期间，在批准的 每个设施的安乐死方法。这项培训包括 无论是成年人还是新生儿。这些技术也将在 实验小鼠训练CD的生物方法学及其应用 NHGRI指南01.1和01.2啮齿动物安乐死和 斑马鱼的安乐死..

Under the OSD, the OLAM supports the research of NHGRI scientists. A critical focus of this support is training in the humane care and handling of research animals. To this end, they have created three training CDs that have enjoyed world-wide distribution: 1) Training in Survival Rodent Surgery, a joint project between the NHGRI and OACU; 2) Training in Basic Biomethodology for Laboratory Mice, a NHGRI funded and produced project that also serves as a backbone for the OACU CD Mouse & Rat Humane Restraint and Experimental Techniques; and 3) Training in Basic Biomethodology for Laboratory Rats, a NHGRI NIH/NHGRI (File 777) 4/08 I-5 project currently under production that has been partially funded by the AALAS Foundation. The training offered through OLAM ensures that all animal handling is in compliance with NHGRI, NIH, and federal regulations and guidelines for the continued assurance of full accreditation by the AAALAC, International. The OLAM also develops and/or recommends refinements in humane care and handling and provides training to researchers in these refinements. In addition to the OACU-required on-line or lecture-based training and registering with AEP, (see NIH overview), the NHGRI ACUC requires additional training prior to being listed on a NHGRI ASP or being added to an ASP by amendment. Each research staff is required to: Complete facility-specific training. This training includes requirements for facility entry, proper animal handling procedures, and other related issues. The APD or her designee performs this training. Review the relevant sections of the CD, Training in Basic Biomethodololgy for Laboratory Mice. This CD was developed and financially supported by NHGRI and covers the most common procedures performed at NHGRI. This includes general husbandry, restraint and handling, identification methods, genotyping, injections, blood collection, anesthesia and analgesia, and euthanasia. The NHGRI Investigator Handbook also offers information on the humane care and use of animals in research. Once the relevant sections of the CD have been reviewed, hands-on, one-on-one training with the APD or her designee occurs to assure that animals are treated humanely without injury to the animal or handler and that skills are appropriate for the experimental manipulation(s) being performed. Individuals performing survival rodent surgery are also required to view the CD, Training in Rodent Survival Surgery developed and financially supported by the NHGRI and the OACU. This CD also covers special considerations in transgenic surgery and basic suturing techniques. Regardless of an investigators skill or training, all surgical manipulations are observed by the APD until she is assured that all aseptic and surgical procedures are being performed properly. If necessary, non-recovery training animals will be used until the surgical technique has been mastered. Sign a statement saying the ASP has been read and the contents are understood. Read the NHGRI ACUC 03.1 LAAPP guideline and attached DOHS allergy brochure. Training is an on-going commitment and a high priority at NHGRI. The OLAM conducts monthly hands-on training sessions in various experimental techniques (e.g., restraint, blood collection, injections, suturing, etc.) The NHGRI APD and ACUC Chairperson also provide updates on new/revised Federal, NIH, and/or NHGRI animal welfare guidelines at the monthly NHGRI faculty meetings. The NHGRI OLAM and the OACU have developed a CD training tool, Training in Survival Rodent Surgery. This CD teaches the user principles of aseptic techniques for rodent surgery, special considerations for transgenic surgery, and simple suture patterns. Viewing of this CD is required for all NHGRI personnel performing rodent survival surgery. Pre-surgical planning is initiated during protocol development. During this time, the surgeons skills and the available facilities/equipment are assessed by the PI and APD. If the investigator is not experienced with the surgical procedure, training with the APD on cadaverous or terminally anesthetized animals is done under the NHGRI training protocol (G-98-1) until the technique has been perfected. Once the protocol is approved, the APD works with the investigator to assure aseptic practices are followed. Regardless of the level of expertise of the investigator, the APD observes all surgeries when they are first performed. This training is documented and maintained in the OLAM trainingfile. In addition, the NHGRI APD provides individualized training as described in the pre-surgical planning section above. Investigator training with the NHGRI APD occurs on an almost daily basis. If the APD lacks the expertise to teach a particular technique, it is the joint responsibility of the APD and the PI to find an individual capable of training the APD and the investigator. All NHGRI investigators using anesthetics are trained by the APD in their safe and effective use and in the proper techniques for induction, maintenance and recovery. This training is documented and maintained in the OLAM training file. These techniques are also reviewed in the Biomethodology for Laboratory Mice training CD. Investigators using inhalant anesthetics are also trained in safe and appropriate scavenging techniques. All NHGRI investigators are trained by the animal facility and the APD, or her designee, during their facility training, in the approved methods of euthanasia for each facility. This training encompasses both adults and neonates. These techniques are also reviewed in the Biomethodology for Laboratory Mice training CD and the NHGRI Guidelines 01.1 and 01.2 Euthanasia of Rodents and Euthanasia of Zebrafish..