High Precision Optical One-to-One Cell Fusion

高精度光学一对一细胞融合

• 批准号: 7728353
• 负责人: Gao Chen
• 金额: $ 20万
• 依托单位: MAXWELL SENSORS, INC.
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-18 至 2011-09-17
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7728353
• 关键词: Antibodies; Antibody Formation; Biotechnology; California; Cell Count; Cell Line; Cell Nucleus; Cell Survival; Cell fusion; Cells; Chemicals; Chinese Hamster; Chinese Hamster Ovary Cell; Cloning; Collaborations; Culture Media; Development; Dimensions; Disease; Dyes; Evaluation; Feasibility Studies; Figs - dietary; Generations; Genes; Genetic Materials; Germ Cells; Goals; Hybrid Cells; Hybridomas; Image; International; Label; Lasers; Lead; Life; Light; Liquid substance; Mammals; Maps; Measures; Methods; Microfluidics; Microscope; Monitor; Monoclonal Antibodies; Multiple Myeloma; Nitrogen; Nuclear; Oils; Operative Surgical Procedures; Optics; Ovary; Pattern; Penicillins; Perforation; Performance; Phase; Photons; Physiologic pulse; Polyethylene Glycols; Price; Process; Property; Protein Biosynthesis; Protocols documentation; Pyruvate; Pyruvates; Receptor Cell; Research Personnel; Sampling; Scanning; Scientist; Shock; Sodium; Solutions; Sorting - Cell Movement; Source; Splenocyte; Spottings; Staining method; Stains; Stem Cell Research; Streptomycin; System; Techniques; Technology; Testing; Time; Toxic effect; Transfection; Ultraviolet Rays; Universities; Vendor; aqueous; base; cancer immunotherapy; cell dimension; cell injury; cell type; cellular imaging; charge coupled device camera; design; electric field; fetal bovine serum; fluorexon; improved; indexing; insight; monoclonal antibody production; novel; optical traps; prototype; public health relevance; research study; sensor; success; tool; voltage

DESCRIPTION (provided by applicant): In the past decade, cell fusion has become a powerful tool for the analysis of hybridoma antibody production, cancer immunotherapy, gene transfection, chromosomal mapping, cloning mammals, and stem cell research. Until now, cell fusion techniques have been based on chemical and physical methods. Using these techniques, however, whole cell damage caused by some types of stimulation was unavoidable and a fusion of specific cells that are required for particular purposes was not possible because of the collective treatment. Therefore, Maxwell Sensors Inc. proposes to develop a novel Optical Cell Fusion (OC-Fusion) chip and system that combines the technologies of picodroplet and optical fusion to rapidly generate hundreds or thousands of hybrid cells with very high precision. The picodroplet method - rapidly and accurately pairs cells in contact with each other in a tiny droplet. A laser - precisely fuses a point at cell contact. The advantages of OC-Fusion are: 1. a versatile droplet technique rapidly pairs 1:1 selected cells in contact inside a picodroplet; the process does not depend on any natural cell contact, specific cell receptors, or labor-intensive optical trapping. 2. hundreds or thousands of hybrid cells can be generated with an image-assisted laser system used to scan the droplet array and precisely fuse the two cells at point contact (< 3<m) with a UV microbeam. In order to achieve the goal of the proposed project, the Phase I specific aims are to design and develop the OC-Fusion chip and system, characterize the system performance, develop protocol, perform and validate cell-cell electrofusion to fuse (1) cultured Chinese hamster ovary (CHO-k1) cells, and (2) splenocyte and myeloma. PUBLIC HEALTH RELEVANCE: Maxwell Sensors Inc. proposes to develop a compact single-cell fusing chip and system that enables to generate hundreds or thousands of fused cells for a variety of biotechnology and biomedical applications. Optical fusion, the process of using laser pulses to fuse two or more living cells, is a growing technology with a wide variety of applications. The technique is integral to cancer immunotherapy, monoclonal antibody generation and stem cell research where it affords researchers greater insight into how genes guide protein synthesis. The technique also allows researchers to create large quantities of different disease-specific antibodies and helps scientists clone mammals.

描述（由申请人提供）：在过去的十年中，细胞融合已成为分析杂交瘤抗体产生、癌症免疫治疗、基因转染、染色体作图、克隆哺乳动物和干细胞研究的强大工具。到目前为止，细胞融合技术一直基于化学和物理方法。然而，使用这些技术时，某些类型的刺激引起的全细胞损伤是不可避免的，并且由于集体治疗，特定目的所需的特定细胞的融合是不可能的。因此，Maxwell Sensors Inc.提出开发一种新型光学细胞融合（OC-Fusion）芯片和系统，结合皮液滴和光学融合技术，以非常高的精度快速生成数百或数千个混合细胞。皮液滴法 - 快速准确地将微小液滴中相互接触的细胞配对。激光 - 精确地熔合细胞接触点。 OC-Fusion 的优点是： 1. 一种多功能液滴技术，可以在皮液滴内快速配对 1:1 的选定细胞并接触；该过程不依赖于任何自然细胞接触、特定细胞受体或劳动密集型光学捕获。 2. 可以使用图像辅助激光系统生成数百或数千个杂交细胞，该系统用于扫描液滴阵列，并使用紫外微束在点接触（< 3< m）处精确地融合两个细胞。为了实现拟议项目的目标，第一阶段的具体目标是设计和开发 OC-Fusion 芯片和系统，表征系统性能，开发协议，执行和验证细胞间电融合以融合（1）培养的中国仓鼠卵巢（CHO-k1）细胞，以及（2）脾细胞和骨髓瘤。公共健康相关性：Maxwell Sensors Inc. 提议开发一种紧凑的单细胞融合芯片和系统，能够为各种生物技术和生物医学应用生成数百或数千个融合细胞。光学融合是使用激光脉冲融合两个或多个活细胞的过程，是一项不断发展的技术，具有广泛的应用。该技术是癌症免疫治疗、单克隆抗体生成和干细胞研究不可或缺的一部分，它使研究人员能够更深入地了解基因如何指导蛋白质合成。该技术还允许研究人员制造大量不同的疾病特异性抗体，并帮助科学家克隆哺乳动物。