Double-Gated Selection of Ligands that Target Surface Markers of Differentiation

针对分化表面标记的配体的双门选择

• 批准号: 7671588
• 负责人: Dana Larocca
• 金额: $ 24.11万
• 依托单位: MANDALA BIOSCIENCES, LLC
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7671588
• 关键词: Alzheimer' s Disease; Astrocytes; Bacteriophages; Cardiac Myocytes; Cell Separation; Cell Therapy; Cell surface; Cells; Degenerative Disorder; Development; Diabetes Mellitus; Differentiation Antigens; EGF gene; Embryo; Epidermal Growth Factor Receptor; Gene Delivery; Gene Expression; Gene Targeting; Gene Transfer; Glial Fibrillary Acidic Protein; Goals; Heart Diseases; In Vitro; Injury; Laboratories; Lead; Libraries; Ligand Binding; Ligands; Macular degeneration; Maps; Masks; Mediating; Methods; Modification; Multiple Sclerosis; Names; Parkinson Disease; Pathway interactions; Peptides; Phage Display; Pluripotent Stem Cells; Population; Preclinical Drug Evaluation; Production; Property; Public Health; Random Peptide Libraries; Reagent; Receptor Cell; Recovery; Replacement Therapy; Reporter Genes; Resistance; Skin; Skin Aging; Source; Specificity; Stem Cell Factor; Stem Cell Research; Stem cells; Surface; System; Technology; Testing; Tissues; WA09 Cell Line; base; cell type; clinical application; drug development; embryonic stem cell; gene transfer vector; human embryonic stem cell; innovation; innovative technologies; nerve stem cell; novel; particle; progenitor; promoter; public health relevance; regenerative; scale up; self-renewal; vector

DESCRIPTION (provided by applicant): Human embryonic stem (hES) cells have the ability to self renew indefinitely and differentiate into virtually any cell type in the body. They offer tremendous potential for the development of new treatments for major degenerative diseases such as heart disease, Alzheimer's disease, Parkinson's, multiple sclerosis, skin aging and macular degeneration, to name a few. Recent developments in reprogramming skin cells to pluripotent cells with the same properties as embryonic stem cells have increased the availability of pluripotent stem cells for research and may ultimately provide cells for clinical applications is they can be proven safe. However, regardless of the source of pluripotent cells, there is a critical unmet need to understand how pluripotent cells differentiate in vitro to facilitate development of efficient methods of directing differentiating of pluripotent cells to desirable mature cell types for cell replacement therapy as well as for drug development. The changes in cell surface molecules that occur as pluripotent stem cells progress toward specification and commitment to various lineages are poorly understood. Mapping the surface of differentiating stem cells using traditional phage display selection methods is problematic on a mixture of heterogenous cells because the more abundant cells and receptors may mask the less common ones. We propose here to apply a novel targeted gene delivery technology combined with display library selection to directly select ligands that target lineage specific progenitors derived from hES cells (WA09). Our unique selection strategy is based on selection of the ligands using 2 gates. To be selected, the ligand-targeted vector must first internalize into the progenitor cells through a surface marker interaction. The second gate is regulated expression of a vector reporter gene in the differentiated cells that are descended from the ligand targeted progenitors. In this manner, only ligands that target progenitors of a specific differentiated cell type like cardiomyocytes as proposed here would be selected. We will first test the concept by using an EGF targeted particle to target neural progenitor cells and genetically selecting targeted particles from astrocytes. After optimizing the vector using the neural progenitor pathway, we will construct a large random peptide library and select it for cardiomyocyte targeting peptides. The selected peptides will be characterized for their ability to target and isolate cardiomyocyte progenitors. Our long term goal is to develop and commercialize targeting ligands and kits for stem cell progenitor targeting, isolation and differentiation into therapeutically useful cell-types and cells for drug screening. PUBLIC HEALTH RELEVANCE: Embryonic and other pluripotent stem cells such as recently discovered reprogrammed skin cells have the ability to self renew indefinitely and differentiate into virtually any cell type in the body. They therefore have the potential to profoundly benefit public health by providing cells for treating virtually any degenerative disease or injury such as heart disease, diabetes, MS, AD, and Parkinson's disease to name a few. It is critical to understand how pluripotent stem cells develop in the laboratory so that the right types of cells can be prepared for safe and effective regenerative treatments. The proposed project will use innovative methods to develop reagents that will facilitate stem cell research and enable the isolation, scale up and production of cells for therapy and drug development.

描述（由申请人提供）：人类胚胎干细胞（hES）具有无限自我更新和分化成体内几乎任何细胞类型的能力。它们为心脏病、阿尔茨海默病、帕金森氏症、多发性硬化症、皮肤老化和黄斑变性等主要退行性疾病的新疗法的开发提供了巨大的潜力。最近在将皮肤细胞重编程为具有与胚胎干细胞相同特性的多能性细胞方面的进展，增加了多能性干细胞用于研究的可用性，并可能最终为临床应用提供细胞，如果它们可以被证明是安全的。然而，无论多能细胞的来源如何，了解多能细胞如何在体外分化，以促进开发有效的方法，指导多能细胞分化为细胞替代疗法和药物开发所需的成熟细胞类型，都是一个关键的未满足的需求。多能干细胞在分化和分化过程中发生的细胞表面分子的变化尚不清楚。利用传统的噬菌体展示选择方法在异质细胞混合物中绘制分化干细胞表面是有问题的，因为更丰富的细胞和受体可能会掩盖不太常见的细胞和受体。我们建议应用一种新的靶向基因传递技术，结合展示文库选择，直接选择靶向来自hES细胞的谱系特异性祖细胞的配体（WA09）。我们独特的选择策略是基于使用2门选择配体。为了被选择，配体靶向载体必须首先通过表面标记相互作用内化到祖细胞中。第二个门是载体报告基因在从配体靶向祖细胞中衍生的分化细胞中的调控表达。通过这种方式，只有针对特定分化细胞类型（如心肌细胞）的祖细胞的配体才会被选择。我们将首先通过使用EGF靶向颗粒靶向神经祖细胞和从星形胶质细胞中遗传选择靶向颗粒来测试这一概念。利用神经祖细胞途径对载体进行优化后，我们将构建一个大的随机肽库，并从中选择心肌细胞靶向肽。所选择的肽将以其靶向和分离心肌细胞祖细胞的能力为特征。我们的长期目标是开发和商业化靶向配体和试剂盒，用于干细胞祖细胞靶向，分离和分化为治疗有用的细胞类型和药物筛选细胞。公共卫生相关性：胚胎和其他多能干细胞，如最近发现的重编程皮肤细胞，具有无限自我更新和分化成体内几乎任何细胞类型的能力。因此，它们有潜力通过提供细胞来治疗几乎任何退行性疾病或损伤，如心脏病、糖尿病、多发性硬化症、阿尔茨海默病和帕金森病，从而深刻地造福公众健康。了解多能干细胞是如何在实验室中发育的至关重要，这样才能为安全有效的再生治疗准备合适类型的细胞。拟议的项目将使用创新方法开发试剂，以促进干细胞研究，并能够分离、扩大和生产用于治疗和药物开发的细胞。