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Core--Histology/ Immunohistochemistry/ In Situ Hybridization

核心--组织学/免疫组织化学/原位杂交

基本信息

批准号：
7575268
负责人：
CHARLES E ALPERS
金额：
$ 14.68万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7575268
关键词：
Address Alkaline Phosphatase Animals Antibodies Biological Assay Biotin Cells Clinical Research Clinical Trials Competitive Binding Consultations DNA DNA Probes Development Diagnostic Digoxigenin Documentation Electron Microscopy Ensure Enzymes Evaluation Freezing Frozen Sections Galactosidase Grant Green Fluorescent Proteins Histocytochemistry Histologic Histology Immunoelectron Microscopy Immunofluorescence Microscopy Immunohistochemistry Immunoperoxidase Technics In Situ Hybridization Indirect Immunofluorescence Intervention Label Localized Microscopy Northern Blotting Organ Paraffin Embedding Pathology Performance Preparation Procedures Process Proteins Purpose RNA RNA Probes Radiolabeled Recruitment Activity Reporter Genes Research Design Research Personnel Sensitivity and Specificity Staining method Stains Standards of Weights and Measures Techniques Tissues Toxic effect United States Food and Drug Administration Universities Washington cell type gene therapy immunocytochemistry improved interest polyclonal antibody preclinical study programs radiotracer sample fixation

项目摘要

The principal purpose of this Core is to provide a common facility and expertise for the broad spectrum of morphologic studies that may be required for interpretation of gene therapy studies. Additional major functions of this Core include the characterization of antibodies and DNA and RNA probes that may be useful for the identification of cell types or localization of molecules of interest in normal and genetically manipulated tissues, and the development of enhancements to existing morphologic techniques that will improve the ability of Center investigators to localize specific molecules in tissue sections. The principal morphologic techniques provided by this Core include routine histology, including special histologic stains, immunohistochemistry, immunofluorescence microscopy, tissue enzyme histochemistry, electron microscopy, immunoelectron microscopy, in situ hybridization, and necessary ancillary techniques such as Western, Southern and Northern blotting and antibody binding and competitive inhibition assays needed to ensure the sensitivity and specificity of all proposed procedures. As the entire gene therapy program at the University of Washington has evolved, a greater emphasis has been placed on pathology analyses of pre-clinical trials, with an appreciation for assistance with nuances of study design and animal toxicity studies that would meet FDA standards for approval to progress to clinical trials. Dr. Denny Liggitt has been recruited into this core to specifically address this need.

本核心的主要目的是为以下方面提供共同的设施和专门知识：解释基因治疗可能需要的广泛的形态学研究问题研究该核心的其他主要功能包括抗体和DNA和RNA探针的表征，这可能有助于在正常和遗传操作的组织中识别细胞类型或定位感兴趣的分子，以及对现有形态学技术的增强，这将提高中心研究者在组织切片中定位特定分子的能力。本核心提供的主要形态学技术包括常规组织学，包括特殊组织学染色、免疫组织化学、免疫荧光显微镜、组织酶组织化学、电子显微镜、免疫电子显微镜、原位杂交和必要的辅助技术，如Western、Southern和北方印迹以及抗体结合和竞争性抑制试验，以确保所有拟定程序的灵敏度和特异性。随着华盛顿大学整个基因治疗项目的发展，更加重视临床前试验的病理学分析，并对研究设计和动物毒性研究的细微差别给予帮助，以满足FDA批准进入临床试验的标准。Denny Liggitt博士已被招募到这个核心，以专门满足这一需求。