Mechanisms of Motion Detection in Retinal Neural Network

视网膜神经网络运动检测机制

• 批准号: 10752012
• 负责人: Tomomi Ichinose
• 金额: $ 46.3万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10752012
• 关键词: Acceleration; Acetylcholine; Affect; Amacrine Cells; Animals; Calcium; Cells; Code; Computer Models; Computer Simulation; Dendrites; Detection; Diameter; Environmental Impact; Environmental Risk Factor; Feedback; Image; Immunohistochemistry; Knowledge; Light; Mediating; Molecular; Motion; Mus; Neural Retina; Neurons; Neurotransmitters; Nicotinic Receptors; Outcome; Pathway interactions; Presynaptic Terminals; Reaction; Reaction Time; Retina; Signal Transduction; Speed; Stimulus; Testing; Tissues; Visual; behavioral study; cholinergic; ganglion cell; meter; motion sensitivity; neural network; neurotransmission; object motion; patch clamp; receptive field; retinal neuron; signal processing; starburst amacrine cell; two-photon

Abstract Detection of moving objects is a retinal function which is crucial for an animal's survival. Multiple neurons and neural networks in the retina have been identified as critical players in this task, including starburst amacrine cells (SACs) and direction-selective ganglion cells (DSGCs), which sense direction of motion. Recent studies have revealed that several neural networks among bipolar and amacrine cells are involved in direction selectivity. However, the impact of environmental factors on motion sensitivity tuning of these neurons is not well understood. Background scenery affects the gain control and tuning of neurons for object motion detection; however, we have just begun to understand the sensitization and adaptation of those neurons. The long-term objective of the present project is to understand the cellular and molecular mechanisms in the retina for sensing direction of motion. We will conduct patch clamp recordings, two-photon calcium imaging, immunohistochemistry, computational simulation, and behavioral studies to examine the mechanisms underlying direction selectivity. We previously found that cholinergic feedback from SACs to bipolar cells contributes to SAC direction selectivity. We now have evidence that the cholinergic feedback is transferred for a long distance and tune SAC direction selectivity. Therefore, we hypothesize that an incoming object send a signal to bipolar cells through a cholinergic pathway to tune SAC direction selectivity, a form of predictive coding. We propose two Specific Aims to investigate long-distance cholinergic feedback. We will test this hypothesis by recording long-distance cholinergic feedback in bipolar cells (Aim 1), and we will examine the outcome of the long-distance cholinergic feedback in bipolar cell axon terminals, SAC dendrites, and DSGC activity (Aim 2). Visual prediction is an essential feature for motion detection, which would reduce neural signal delays and facilitate the animal reaction. Knowledge gained from the results of this project will shed light on the additional layer of motion detection and visual signal processing in the retina.

摘要

项目成果

ON and OFF Signaling Pathways in the Retina and the Visual System.

视网膜和视觉系统中的开和关信号通路。

• DOI: 10.3389/fopht.2022.989002
• 发表时间: 2022
• 期刊: Frontiers in ophthalmology
• 作者: Ichinose,Tomomi;Habib,Samar
• 通讯作者: Habib,Samar

Using Looming Visual Stimuli to Evaluate Mouse Vision.

• DOI: 10.3791/59766
• 发表时间: 2019-06
• 期刊: Journal of visualized experiments : JoVE
• 作者: Christina C. Koehler;Leo M Hall;Chase B. Hellmer;T. Ichinose

Patch clamp recording from bipolar cells in the wholemount mouse retina.

• DOI: 10.1016/j.xpro.2022.101482
• 发表时间: 2022-09-16
• 期刊: STAR protocols

Presynaptic depolarization differentially regulates dual neurotransmitter release from starburst amacrine cells in the mouse retina.

突触前去极化差异调节小鼠视网膜星爆无长突细胞的双重神经递质释放。

• DOI: 10.3389/fopht.2023.1225824
• 发表时间: 2023
• 期刊: Frontiers in ophthalmology
• 作者: Ichinose,Tomomi;Hellmer,ChaseB;Bohl,JeremyM
• 通讯作者: Bohl,JeremyM