Noninvasive Measurement Of Strain And Mechanical Properties In Tendons/Ligaments

肌腱/韧带应变和机械性能的无创测量

• 批准号: 7840353
• 负责人: RAY VANDERBY
• 金额: $ 21.67万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-15 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7840353
• 关键词: Accounting; Acoustics; Algorithms; American Cancer Society; Animal Model; Animals; Area; Arteries; Behavior; Benign; Biomechanics; Clinical; Cost Savings; Data; Defect; Degenerative polyarthritis; Development; Diagnosis; Diagnostic; Diagnostic Neoplasm Staging; Diagnostic Procedure; Disabled Persons; Failure; Family suidae; Flexor; Healed; Histocompatibility Testing; Image; In Situ; In Vitro; Investigation; Joint Laxity; Knee; Knowledge; Ligaments; Limb structure; Magnetic Resonance Imaging; Maintenance; Malignant - descriptor; Malignant neoplasm of prostate; Mammary Neoplasms; Maps; Marfan Syndrome; Measurement; Measures; Mechanics; Medical; Medicare; Methods; Modeling; Mus; Musculoskeletal; National Institute of Arthritis and Musculoskeletal and Skin Diseases; Operative Surgical Procedures; Orthopedics; Pathologic; Population; Property; Public Health; Publishing; Rattus; Recovery; Rehabilitation therapy; Research Personnel; Resolution; Rupture; Shoulder; Signal Transduction; Simulate; Skin; Specimen; Sprain; Stress; Stretching; Surgeon; System; Techniques; Technology; Tendinitis; Tendon structure; Testing; Time; Tissues; Tumor stage; Ultrasonic wave; Ultrasonography; Visit; Woman; achilles tendon; base; clinically relevant; cost; efficacy testing; handicapping condition; healing; improved; in vivo; innovation; interest; ligament injury; malignant breast neoplasm; mathematical theory; men; musculoskeletal imaging; novel diagnostics; programs; public health relevance; soft tissue; theories; tool; tumor

DESCRIPTION (provided by applicant): We propose a new paradigm for ultrasound wave analysis in order to non-invasively compute mechanical properties (nonlinear stiffness) and functional strains in ligaments and tendons. Mechanical properties can diagnose damage or a pathologic state (i.e. tendonitis or tendinosis) and it can quantify the extent of healing. Tissue strains indicate functional loadings for rehabilitation and recovery. If successful, our method will significantly improve upon existing methods and find numerous applications that benefit public health. And, it will provide an improved tool for scientific inquiry in many musculoskeletal studies of relevance to NIAMS. This study applies acoustoelasticity (AE) to biomedical ultrasound imaging for the first time. AE is a mathematical theory that rigorously describes ultrasonic wave propagation in deformed elastic materials, interrelating reflected waves to mechanical properties and strain. After a tissue such as tendon or ligament is stretched (i.e. functionally loaded), AE models wave propagation in a deformed medium more accurately than existing methods. Currently, other ultrasound-based methods use "wave theory", which does not account for deformation-dependent changes in wave velocity and amplitude. Preliminary studies clearly show that "wave theory" analysis can cause significant errors when ultrasound is used to compute tissue properties. AE analysis has the potential to: 1) avoid these errors, 2) include tissue non-linearities, 3) acquire all requisite data with one functional loading, and 4) compute strain and mechanical properties in virtually real time. This technique does not require additional force measurements or time-consuming numerical modeling to extract properties. To our knowledge, no existing method can evaluate both applied strain and nonlinear tissue properties simultaneously in virtually real time. This study will test the efficacy of an AE-based technique termed "Acoustoelastic Strain Gauge" (ASG) on animal tendons (tendons in rat and pig limbs after sacrifice). Tendons will be analyzed in situ, that is, through skin and superficial tissues while being stretched with a mechanical test system. In this study ASG will be applied to porcine flexor tendons (intact and with induced subfailure damage to simulate various levels of 2nd degree sprains). ASG will be applied to rat Achilles tendons (intact and after surgical rupture and various periods of healing). In both animal models, applied strain and strain-dependent tissue properties of the in situ stretched tendons will be evaluated. Strain from ASG will be correlated with strain from a mechanical test system to demonstrate accuracy and repeatability of the ASG technique. This study will determine the ability and sensitivity of ASG to quantify the level of sub-failure damage (as determined by a reduction in ultimate force). Finally, this study will determine the ability of ASG to predict strength recovery during healing. PUBLIC HEALTH RELEVANCE: We propose a new and different method of ultrasound wave analysis to non-invasively measure mechanical properties and functional strains in soft tissues. If successful, this new diagnostic tool be a significant improvement over existing methods and will find numerous applications that benefit public health. Consider, for example, functional strain measurement. In the U.S. more than 12 million people visited orthopedic surgeons in 2003 because of either knee or shoulder problems. Knee ligament injuries are common, and almost all shoulder problems are related to stretched or ruptured tissues with altered functional loads. Consider, for example, mechanical property identification. The American Cancer Society estimated in 2005 that approximately 211,240 women were diagnosed with invasive breast cancer in the U.S. and 232,090 men were diagnosed with prostate cancer. Clearly, non-invasive tissue strain to measure functional loads and non-invasive mechanical properties to distinguish and identify tissue types would be valuable. Our method has the potential to improve upon existing methods in these areas. A recent study in a Medicare population projected that musculoskeletal imaging costs in 2020 will be $3.6 billion, of which $2 billion will be for MRI. Investigators estimated 45% of the MRI diagnoses could have been made with ultrasound, and if appropriately substituted, could result in cost savings of nearly $7 billion over the next 14 years. Innovative developments in ultrasound technology may create value added features resulting in increased interest and significant.

描述（由申请人提供）：我们提出了一种新的超声波分析范例，以便非侵入性地计算韧带和肌腱的机械特性（非线性刚度）和功能应变。机械特性可以诊断损伤或病理状态（即肌腱炎或肌腱变性），并且可以量化愈合程度。组织应变表明康复和恢复的功能负荷。如果成功，我们的方法将显着改进现有方法，并找到许多有益于公共健康的应用。而且，它将为与 NIAMS 相关的许多肌肉骨骼研究中的科学探究提供改进的工具。 该研究首次将声弹性（AE）应用于生物医学超声成像。 AE 是一种数学理论，严格描述超声波在变形弹性材料中的传播，将反射波与机械性能和应变联系起来。在肌腱或韧带等组织被拉伸（即功能加载）后，AE 比现有方法更准确地模拟变形介质中的波传播。目前，其他基于超声的方法使用“波理论”，该理论没有考虑波速和振幅的变形相关变化。初步研究清楚地表明，当使用超声波计算组织特性时，“波理论”分析可能会导致重大错误。 AE 分析有潜力：1) 避免这些误差，2) 包括组织非线性，3) 通过一种功能载荷获取所有必需的数据，4) 几乎实时计算应变和机械特性。该技术不需要额外的力测量或耗时的数值建模来提取属性。据我们所知，没有任何现有方法可以几乎实时地同时评估施加的应变和非线性组织特性。这项研究将测试一种名为“声弹性应变仪”(ASG) 的基于 AE 的技术对动物肌腱（处死后大鼠和猪四肢的肌腱）的功效。肌腱将在原位进行分析，即在用机械测试系统拉伸时通过皮肤和浅表组织进行分析。在本研究中，ASG 将应用于猪屈肌腱（完整且具有诱导的亚失效损伤，以模拟各种级别的二度扭伤）。 ASG 将应用于大鼠跟腱（完整的、手术破裂后和不同愈合期的）。在这两种动物模型中，将评估原位拉伸肌腱的施加应变和应变依赖性组织特性。 ASG 的应变将与机械测试系统的应变相关联，以证明 ASG 技术的准确性和可重复性。这项研究将确定 ASG 量化次失效损坏水平（通过极限力的减少确定）的能力和灵敏度。最后，这项研究将确定 ASG 预测愈合过程中力量恢复的能力。 公共健康相关性：我们提出了一种新的、不同的超声波分析方法，用于非侵入性测量软组织的机械特性和功能应变。如果成功，这种新的诊断工具将是对现有方法的重大改进，并将发现许多有益于公众健康的应用。例如，考虑功能应变测量。 2003年，美国有超过1200万人因为膝盖或肩部问题去看整形外科医生。膝关节韧带损伤很常见，几乎所有肩部问题都与功能负荷改变的组织拉伸或破裂有关。例如，考虑机械性能识别。美国癌症协会 2005 年估计，美国大约有 211,240 名女性被诊断出患有浸润性乳腺癌，232,090 名男性被诊断出患有前列腺癌。显然，用于测量功能负荷的非侵入性组织应变和用于区分和识别组织类型的非侵入性机械特性将是有价值的。我们的方法有潜力改进这些领域的现有方法。最近一项针对医疗保险人群的研究预测，2020 年肌肉骨骼成像费用将达到 36 亿美元，其中 20 亿美元用于 MRI。研究人员估计 45% 的 MRI 诊断可以通过超声波进行，如果适当替代，可以在未来 14 年内节省近 70 亿美元的成本。超声技术的创新发展可能会创造增值功能，从而引起人们的兴趣和意义。

项目成果

Shear loads induce cellular damage in tendon fascicles.

剪切载荷会引起肌腱束的细胞损伤。

• DOI: 10.1016/j.jbiomech.2015.06.006
• 发表时间: 2015
• 期刊: Journal of biomechanics
• 影响因子: 2.4
• 作者: Kondratko-Mittnacht,Jaclyn;Lakes,Roderic;VanderbyJr,Ray
• 通讯作者: VanderbyJr,Ray

Time-dependent ultrasound echo changes occur in tendon during viscoelastic testing.

在粘弹性测试期间，肌腱中会发生随时间变化的超声回波变化。

• DOI: 10.1115/1.4007745
• 发表时间: 2012
• 期刊: Journal of biomechanical engineering
• 作者: Duenwald-Kuehl,Sarah;Kobayashi,Hirohito;Lakes,Roderic;VanderbyJr,Ray
• 通讯作者: VanderbyJr,Ray

Quantification of collagen organization and extracellular matrix factors within the healing ligament.

• DOI: 10.1017/s1431927611011925
• 发表时间: 2011-10
• 期刊: MICROSCOPY AND MICROANALYSIS
• 影响因子: 2.8
• 作者: Chamberlain, Connie S.;Crowley, Erin M.;Kobayashi, Hirohito;Eliceiri, Kevin W.;Vanderby, Ray
• 通讯作者: Vanderby, Ray

Mechanical compromise of partially lacerated flexor tendons.

部分撕裂的屈肌腱的机械损伤。

• DOI: 10.1115/1.4023092
• 发表时间: 2013
• 期刊: Journal of biomechanical engineering
• 作者: Kondratko,Jaclyn;Duenwald-Kuehl,Sarah;Lakes,Roderic;VanderbyJr,Ray
• 通讯作者: VanderbyJr,Ray

Shear load transfer in high and low stress tendons.

• DOI: 10.1016/j.jmbbm.2015.01.021
• 发表时间: 2015-05
• 期刊: Journal of the mechanical behavior of biomedical materials
• 影响因子: 3.9
• 作者: Kondratko-Mittnacht J;Duenwald-Kuehl S;Lakes R;Vanderby R Jr
• 通讯作者: Vanderby R Jr