Plasmon-Controlled Fluorescence and Cardiac Markers

描述(由申请人提供)：竞争性修订血浆蛋白控制的荧光和心脏标记物。这是针对通知编号NOT-OD-09-058的竞争修订申请。母公司资助EB 006521的目标是使用理论、纳米制造和光谱学的组合来开发定义的纳米结构，从而从表面结合的荧光团产生增强的和/或定向的荧光。这些已定义的结构随后被用于开发心脏标志物的生物检测。虽然这项工作正在进行中，我们意识到需要更快地在其他实验室的研究中实施等离子控制荧光，并在生物技术和临床诊断学中实施。在这里，我们建议加快使用批量化学方法来制造金属纳米结构的研究，这可以在其他实验室进行。我们发现，这些高密度粒子为利用寿命和极化检测模式的新方法开发生物活性表面提供了极好的机会。当使用粒子等离子激元产生的高度放大的荧光信号时，寿命和偏振测量的实现是独一无二的，并为将荧光应用显著扩展到实时研究生物分子相互作用和开发新的生物分析格式提供了机会。扩展的具体目标：具体目标1.采用间歇化学合成方法制备表面密度不同的金属纳米颗粒(MNPs)玻璃基板。确定哪些MNPs构型最适合强度、寿命和荧光去偏振的变化。具体目的2.基于MNPs对强度、寿命和极化的影响，开发多种心脏标志物的检测方法。为了实现扩展的具体目标，我们将聘请两名新的博士级别的科学家。一名化学家，最好在金属颗粒合成和金属表面化学方面有经验。第二个人将是一名生物化学家，最好有荧光光谱学经验。 公共卫生相关性：PCF心脏竞争性修订该项目的目标是开发结合使用荧光、近场光学和纳米制造来检测心脏标记物的新方法。这一竞争性修订申请的目的是加速将我们的方法引入实际应用，这将使用制造方法来完成，制造方法可以使用自下而上的合成和组装来完成。这一具体目标的扩展将导致雇用两名博士级别的研究助理。

DESCRIPTION (provided by applicant): Competitive Revision Plasmon Controlled Fluorescence and Cardiac Markers. This is a Competitive Revision Application in response to Notice Number NOT-OD-09-058. The objectives of the parent grant EB 006521 are to use a combination of theory, nanofabrication and spectroscopy to develop defined nanostructures which result in enhanced and/or directional fluorescence from surface-bound fluorophores. These defined structures are then being used for development of bioassays for the cardiac markers. While this work is in progress, we were made aware of the need for more rapid implementation of plasmon-controlled fluorescence into research in other laboratories and implementation into biotechnology and clinical diagnostics. Here we propose to expedite the research using batch chemical methods for fabrication of metallic nanostructures which can be performed in other laboratories. We have found that these high density particles provide an excellent opportunity for development of bioactive surfaces using new approaches with lifetime and polarization detection modalities. The implementation of lifetime and polarization measurements are unique when using highly amplified fluorescence signal due to particle plasmons and provide opportunity for significantly expanding the fluorescence applications to studies of biomolecules interactions in real-time and development of new formats for bioassays. The extended Specific Aims: Specific Aim 1. Use batch chemical synthesis methods to make glass substrates with metal nanoparticles (MNPs) with various surface density. Identify which MNPs geometries are best suited for changes in intensity, lifetime and fluorescence depolarization. Specific Aim 2. Develop assays for multiple cardiac markers based on the effects of MNPs on intensity, lifetime and polarization. To accomplish the extended Specific Aims we will hire two new Ph.D. level scientists. One individual will be a chemist preferably with experience in the synthesis of metal particles and metal surface chemistry. The second individual will be a biochemist preferably with experience in fluorescence spectroscopy. PUBLIC HEALTH RELEVANCE: PCF Cardiac Competitive Revision The goal of this project is to develop new methods for detection of cardiac markers using a combination of fluorescence, near-field optics and nanofabrication. The purpose of this Competitive Revision application is to speed the introduction of our methods into practical applications this will be accomplished using fabrication methods which can be accomplished using bottom-up synthesis and assembly. This extension of the specific aims will result in the hiring of two PhD-level research associates.