Multi-User Time-Resolved Fluorescence Spectrometer

多用户时间分辨荧光光谱仪

• 批准号: 8825781
• 负责人: Joseph R. LAKOWICZ
• 金额: $ 24.34万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8825781
• 关键词: Anisotropy; Baltimore; Biochemistry; Biological; Collection; Computer software; Computers; Contract Services; Event; Fees; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Fluorescence Spectroscopy; Frequencies; Funding; Grant; Maryland; Measurement; Methods; Minor; Molecular Biology; Optics; Peer Review; Principal Investigator; Publications; Research; Research Personnel; Scientist; Time; United States National Institutes of Health; Universities; base; design; instrument; instrumentation; medical schools; optical spectra; tau Proteins

DESCRIPTION (provided by applicant): The Center for Fluorescence Spectroscopy (CFS) has provided time-resolved measurements to scientists on this campus since 1988, resulting in over 200 peer-reviewed publications. This instrumentation is now obsolete or nonfunctional. NIH-funded principal investigators (PIs) at the University of Maryland, Baltimore (UMB) have a scientific need for a state-of-the-art fluorescence lifetime spectrofluorometer to support and enhance their NIH funded research. The requested instrument is a modern recently developed computer controlled spectrofluorometer which performs automated time-resolved measurements. The FluoTime 300 "Easy Tau" is a new instrument from PicoQuant designed to make time-resolved measurements easily available to biological researchers. This instrument will be located in and managed by the Center for Fluorescence Spectroscopy (CFS) at UMB and will be available for PIs throughout UMB. This instrument will provide automated collection of fluorescence intensity, fluorescence polarization (FP), anisotropy decays, time- resolved emission spectra, decay-associated spectra, and fluorescence resonance energy transfer (FRET). At present we have limited capabilities for time-resolved measurements. We have one LED-based frequency- domain (FD) instrument with very low sensitivity and one time-domain instrument which uses only optical filters for wavelength selection. These instruments can only be operated by spectroscopists and cannot support the demand for time-resolved measurements of UMB. The CFS has extensive software for time-resolved fluorescence using the frequency-domain method. With support from the Department of Biochemistry and Molecular Biology this valuable software will be modified for TCSPC measurements. The School of Medicine guarantees support for the service contract for five years in the event of a shortage from user fees. The PicoQuant FT300 will be used 100% of the time by 10 major users supported by 14 NIH grants and by 8 minor users supported by 3 NIH grants.

描述（由申请人提供）：荧光光谱中心（CFS）自1988年以来一直为该校区的科学家提供时间分辨测量，导致200多篇同行评议的出版物。这种仪器现在已经过时或不起作用。位于巴尔的摩的马里兰州大学（UMB）的NIH资助的主要研究者（PI）对最先进的荧光寿命分光荧光计有科学需求，以支持和增强其NIH资助的研究。所要求的仪器是一个现代最近开发的计算机控制的荧光分光光度计，进行自动时间分辨测量。FluoTime 300“Easy Tau”是PicoQuant的一款新仪器，旨在为生物研究人员提供轻松的时间分辨测量。该仪器将位于UMB的荧光光谱中心（CFS）并由其管理，并可供整个UMB的PI使用。该仪器将提供荧光强度、荧光偏振（FP）、各向异性衰减、时间分辨发射光谱、衰减相关光谱和荧光共振能量转移（FRET）的自动收集。目前，我们的时间分辨测量能力有限。我们有一个基于LED的频域（FD）仪器具有非常低的灵敏度和一个时域仪器，其仅使用光学滤波器进行波长选择。这些仪器只能由光谱学家操作，不能支持UMB时间分辨测量的需求。CFS拥有使用频域方法进行时间分辨荧光的广泛软件。在生物化学和分子生物学系的支持下，这一有价值的软件将被修改用于TCSPC测量。医学院保证在用户费用短缺的情况下支持服务合同五年。PicoQuant FT300将由14个NIH赠款的10个主要用户和3个NIH赠款的8个次要用户100%使用。