Multi-User Time-Resolved Fluorescence Spectrometer

多用户时间分辨荧光光谱仪

• 批准号: 8825781
• 负责人: Joseph R. LAKOWICZ
• 金额: $ 24.34万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8825781
• 关键词: Anisotropy; Baltimore; Biochemistry; Biological; Collection; Computer software; Computers; Contract Services; Event; Fees; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Fluorescence Spectroscopy; Frequencies; Funding; Grant; Maryland; Measurement; Methods; Minor; Molecular Biology; Optics; Peer Review; Principal Investigator; Publications; Research; Research Personnel; Scientist; Time; United States National Institutes of Health; Universities; base; design; instrument; instrumentation; medical schools; optical spectra; tau Proteins

DESCRIPTION (provided by applicant): The Center for Fluorescence Spectroscopy (CFS) has provided time-resolved measurements to scientists on this campus since 1988, resulting in over 200 peer-reviewed publications. This instrumentation is now obsolete or nonfunctional. NIH-funded principal investigators (PIs) at the University of Maryland, Baltimore (UMB) have a scientific need for a state-of-the-art fluorescence lifetime spectrofluorometer to support and enhance their NIH funded research. The requested instrument is a modern recently developed computer controlled spectrofluorometer which performs automated time-resolved measurements. The FluoTime 300 "Easy Tau" is a new instrument from PicoQuant designed to make time-resolved measurements easily available to biological researchers. This instrument will be located in and managed by the Center for Fluorescence Spectroscopy (CFS) at UMB and will be available for PIs throughout UMB. This instrument will provide automated collection of fluorescence intensity, fluorescence polarization (FP), anisotropy decays, time- resolved emission spectra, decay-associated spectra, and fluorescence resonance energy transfer (FRET). At present we have limited capabilities for time-resolved measurements. We have one LED-based frequency- domain (FD) instrument with very low sensitivity and one time-domain instrument which uses only optical filters for wavelength selection. These instruments can only be operated by spectroscopists and cannot support the demand for time-resolved measurements of UMB. The CFS has extensive software for time-resolved fluorescence using the frequency-domain method. With support from the Department of Biochemistry and Molecular Biology this valuable software will be modified for TCSPC measurements. The School of Medicine guarantees support for the service contract for five years in the event of a shortage from user fees. The PicoQuant FT300 will be used 100% of the time by 10 major users supported by 14 NIH grants and by 8 minor users supported by 3 NIH grants.

描述(由申请人提供)：自1988年以来，荧光光谱中心(CFS)为该校的科学家提供了时间分辨测量，发表了200多篇同行评议的出版物。此仪器现在已过时或不起作用。美国国立卫生研究院资助的巴尔的摩马里兰大学(UMB)首席研究人员(PI)在科学上需要一种最先进的荧光寿命光谱荧光仪来支持和加强他们由NIH资助的研究。所要求的仪器是最近开发的一种现代计算机控制的荧光分光光度计，它执行自动的时间分辨测量。FluoTime 300“Easy Tau”是PicoQuant的一种新仪器，旨在使生物研究人员能够轻松地进行时间分辨测量。该仪器将设在UMB的荧光光谱中心(CFS)并由其管理，并将在整个UMB供PI使用。该仪器将提供荧光强度、荧光偏振(FP)、各向异性衰减、时间分辨发射光谱、衰减相关光谱和荧光共振能量转移(FRET)的自动采集。目前，我们在时间分辨测量方面的能力有限。我们有一个灵敏度非常低的基于LED的频域(FD)仪器和一个只使用滤光片选择波长的时域仪器。这些仪器只能由光谱仪操作，不能支持对UMB的时间分辨测量的需求。CFS拥有广泛的软件，可以使用频域方法进行时间分辨荧光。在生物化学和分子生物学系的支持下，这个有价值的软件将被修改以用于TCSPC测量。医学院保证，在用户费用短缺的情况下，为服务合同提供五年的支持。PicoQuant Ft300将100%由10个主要用户使用，10个主要用户由14个NIH拨款支持，8个小用户由3个NIH拨款支持。