Multi-User Time-Resolved Fluorescence Spectrometer

多用户时间分辨荧光光谱仪

• 批准号: 8825781
• 负责人: Joseph R. LAKOWICZ
• 金额: $ 24.34万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-03-01 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8825781
• 关键词: Anisotropy; Baltimore; Biochemistry; Biological; Collection; Computer software; Computers; Contract Services; Event; Fees; Fluorescence; Fluorescence Polarization; Fluorescence Resonance Energy Transfer; Fluorescence Spectroscopy; Frequencies; Funding; Grant; Maryland; Measurement; Methods; Minor; Molecular Biology; Optics; Peer Review; Principal Investigator; Publications; Research; Research Personnel; Scientist; Time; United States National Institutes of Health; Universities; base; design; instrument; instrumentation; medical schools; optical spectra; tau Proteins

DESCRIPTION (provided by applicant): The Center for Fluorescence Spectroscopy (CFS) has provided time-resolved measurements to scientists on this campus since 1988, resulting in over 200 peer-reviewed publications. This instrumentation is now obsolete or nonfunctional. NIH-funded principal investigators (PIs) at the University of Maryland, Baltimore (UMB) have a scientific need for a state-of-the-art fluorescence lifetime spectrofluorometer to support and enhance their NIH funded research. The requested instrument is a modern recently developed computer controlled spectrofluorometer which performs automated time-resolved measurements. The FluoTime 300 "Easy Tau" is a new instrument from PicoQuant designed to make time-resolved measurements easily available to biological researchers. This instrument will be located in and managed by the Center for Fluorescence Spectroscopy (CFS) at UMB and will be available for PIs throughout UMB. This instrument will provide automated collection of fluorescence intensity, fluorescence polarization (FP), anisotropy decays, time- resolved emission spectra, decay-associated spectra, and fluorescence resonance energy transfer (FRET). At present we have limited capabilities for time-resolved measurements. We have one LED-based frequency- domain (FD) instrument with very low sensitivity and one time-domain instrument which uses only optical filters for wavelength selection. These instruments can only be operated by spectroscopists and cannot support the demand for time-resolved measurements of UMB. The CFS has extensive software for time-resolved fluorescence using the frequency-domain method. With support from the Department of Biochemistry and Molecular Biology this valuable software will be modified for TCSPC measurements. The School of Medicine guarantees support for the service contract for five years in the event of a shortage from user fees. The PicoQuant FT300 will be used 100% of the time by 10 major users supported by 14 NIH grants and by 8 minor users supported by 3 NIH grants.

描述（由申请人提供）：自 1988 年以来，荧光光谱中心 (CFS) 一直为该校园的科学家提供时间分辨测量，产生了 200 多篇同行评审的出版物。该仪器现已过时或无法使用。巴尔的摩马里兰大学 (UMB) 的 NIH 资助的首席研究员 (PI) 迫切需要最先进的荧光寿命分光荧光计来支持和加强他们的 NIH 资助的研究。所需的仪器是最近开发的现代计算机控制分光荧光计，可进行自动时间分辨测量。 FluoTime 300“Easy Tau”是 PicoQuant 的一款新型仪器，旨在使生物研究人员轻松获得时间分辨测量。该仪器将位于 UMB 荧光光谱中心 (CFS) 并由其管理，并且可供整个 UMB 的 PI 使用。该仪器将自动收集荧光强度、荧光偏振 (FP)、各向异性衰变、时间分辨发射光谱、衰变相关光谱和荧光共振能量转移 (FRET)。目前我们的时间分辨测量能力有限。我们拥有一台灵敏度极低的基于 LED 的频域 (FD) 仪器和一台仅使用光学滤波器进行波长选择的时域仪器。这些仪器只能由光谱学家操作，无法支持 UMB 时间分辨测量的需求。 CFS 拥有丰富的软件，可使用频域方法进行时间分辨荧光。在生物化学和分子生物学系的支持下，这一有价值的软件将针对 TCSPC 测量进行修改。如果使用费短缺，医学院将保证五年内对服务合同的支持。 PicoQuant FT300 将 100% 被由 14 项 NIH 拨款支持的 10 位主要用户和由 3 项 NIH 拨款支持的 8 名次要用户使用。