Purchase of a Triple Quadrupole Mass Spectrometer for Multiple Reaction Monitorin

描述（由申请人提供）：本申请申请资金用于三重四极杆质谱仪，该质谱仪具有独特的功能，可以使用称为多重反应监测（MRM）的方法定量特定蛋白质和其他生物分子。一组非常有经验的研究人员，目前有许多用于鉴定和表征蛋白质结构的高分辨率串联质谱仪，将使用该仪器和MRM技术进行需要相当高的灵敏度和大量样品定量测量的目标分析。四个实验室将构成主要用户：Robert J. Cotter领导的中大西洋质谱（MAMS）实验室，Robert Cole领导的约翰霍普金斯SOM质谱蛋白质组学设施，以及Akhilesh Pandey和Gerald Hart的研究实验室。主要项目还包括与MAMS和MS/蛋白质组学实验室直接合作的一些研究人员。对于一些建议使用该仪器的项目，MRM提供了比iTRAQ测量更敏感和选择性的方法，目前该研究小组在遗传学，唐氏综合症，艾滋病毒和痴呆症，糖尿病和肺癌，胰腺癌和肝癌的研究中使用了这种测量方法。此外，该仪器的恒定中性损失（CNL）和前体离子扫描模式将用于筛选泛素化和SUMOylated蛋白以及与氧化应激相关的亚硝基化蛋白生物标志物。公共卫生相关性：本申请申请一种质谱仪，能够从大量样品中筛选具有高灵敏度的特定蛋白质和其他生物分子。例如，由疾病和对照个体组成的大型患者队列的结果可用于识别疾病诊断的生物标志物，用于监测治疗结果，或用于建立设计新药所需的生化途径。这组研究人员将利用该仪器进行遗传学、唐氏综合症、艾滋病毒和痴呆症、糖尿病以及肺癌、胰腺癌和肝癌的研究。

DESCRIPTION (provided by applicant): This application requests funds for a triple quadrupole mass spectrometer with unique capabilities for quantitating specific proteins and other biological molecules using an approach known as multiple reaction monitoring (MRM). A group of very experienced investigators, with access currently to a number of high resolution and tandem mass spectrometers for identifying and characterizing protein structures, will use this instrument and the MRM technique for targeted analyses requiring considerably higher sensitivity and quantitative measurements from large numbers of samples. Four laboratories will constitute the major users: the Middle Atlantic Mass Spectrometry (MAMS) Laboratory directed by Robert J. Cotter, the Johns Hopkins SOM Mass Spectrometry Proteomics Facility directed by Robert Cole, and the research laboratories of Akhilesh Pandey and Gerald Hart. Major projects also include a number of investigators collaborating directly with the MAMS and MS/Proteomics laboratories. For a number of the projects proposed to utilize this instrument, MRM provides a more sensitive and selective approach than the iTRAQ measurements currently used by this group of investigators in studies on genetics, Down syndrome, HIV and dementia, diabetes and cancers of the lung, pancreas and liver. Additionally, the constant neutral loss (CNL) and precursor ion scan modes of this instrument will be used to screen for ubiquitylated and SUMOylated proteins and for nitrosylated protein biomarkers associated with oxidative stress. PUBLIC HEALTH RELEVANCE: This application requests funds for a mass spectrometer capable of screening for specific proteins and other biological molecules with high sensitivity from large numbers of samples. The results from, for example, a large patient cohort composed of disease and control individuals may be used to identify biomarkers for disease diagnosis, for monitoring the outcomes of therapies, or to establish the biochemical pathways needed to design new drugs. This group of investigators will utilize the instrument in studies on genetics, Down syndrome, HIV and dementia, diabetes and cancers of the lung, pancreas and liver.