Becton Dickinson LSR II Flow Cytometer

Becton Dickinson LSR II 流式细胞仪

• 批准号: 7594876
• 负责人: BRUCE J SHENKER
• 金额: $ 27.38万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7594876
• 关键词: 20 year old; Air; Area; Arts; Biology; Calcium; Cell Cycle; Cells; Communicable Diseases; Connective Tissue; Contract Services; Core Facility; Dental Schools; Disease; Equipment; Flow Cytometry; Fluorescence; Fluorochrome; Funding; Future; Immunity; Individual; Infection; Inflammation; Ions; Knowledge; Laboratories; Lasers; Lipids; Manufacturer Name; Medicine; Metabolism; Monitor; Mouth Diseases; Oral; Oral health; Pathogenesis; Pathologic; Periodontal Diseases; Physiological; Proteins; Research; Research Activity; Research Personnel; Research Support; Scientist; Signal Pathway; Stimulus; Surface; Technology; Tissues; United States National Institutes of Health; cell analyzer; craniofacial; flexibility; instrument; malignant mouth neoplasm; meetings; oral tissue; public health relevance; research facility; response; success

DESCRIPTION (provided by applicant): The short and long-term objective of this proposal is to upgrade the School of Dental Medicine (SDM) Flow Cytometry Core Facility so that it may continue to operate with state-of-the-art equipment. The facility is currently equipped with a 20 year-old instrument that can no longer be maintained on service contracts as it has been designated as obsolete by the manufacturer. Funds are requested to purchase a high-end flow cytometer; specifically, we plan to purchase a Becton Dickinson LSR II cell analyzer. The BD LSR II is an air-cooled, four laser (488nm, 633nm, 405nm and 355 nm) benchtop flow cytometer with the capability of acquiring 10 fluorescence parameters as configured. This configuration will provide the flexibility to meet the diverse needs of our user group. Moreover, it will support our investigators as they expand their studies to larger numbers of fluorochromes and thereby obtain significantly more information per cell. Investigators will be able to monitor: a wide range of both surface and intracellular markers, assess changes in cellular concentrations of ions such as calcium, cell cycle distribution, signaling pathways as well as study protein-protein and protein-lipid interactions. Core research facilities are vital to the success of research enterprises such as that at the SDM by providing investigators with access to advanced technologies in their research activities, which for financial reasons, would otherwise not be affordable to the individual scientist or laboratory. The SDM Flow Cytometry Facility supports the diverse needs of over 11 scientists whose research efforts are focused on advancing oral health. Supported projects are focused, in particular, on studies on the response of oral and craniofacial tissue to physiologic and pathologic stimuli with emphasis in three areas: inflammation and immunity, infectious disease, and the biology of soft and hard connective tissue. Public Health Relevance: The SDM Flow Cytometry Facility and the requested funds for acquisition of a state-of-the-art flow cytometer are vital to ongoing studies that elucidate pathogenic mechanisms in periodontal disease, herpetic infection, oral cancer and disorders associated with the metabolism of oral tissues, among others. Of particular relevance, this facility provides the necessary support for studies that are currently funded by the NIH as well as provide for an advantage for our investigators as they compete for future federal research support to advance our knowledge of the pathogenesis of oral disease.

描述（由申请人提供）：该提案的短期和长期目标是升级牙科医学院（SDM）流式细胞术核心设施，使其能够继续使用最先进的设备运行。该设施目前配备了一台已有 20 年历史的仪器，由于制造商已将其指定为过时仪器，因此无法再根据服务合同进行维护。需要资金购买高端流式细胞仪；具体来说，我们计划购买 Becton Dickinson LSR II 细胞分析仪。 BD LSR II 是一款风冷四激光（488nm、633nm、405nm 和 355 nm）台式流式细胞仪，能够根据配置采集 10 个荧光参数。这种配置将提供灵活性，以满足我们用户组的多样化需求。此外，它将支持我们的研究人员将研究扩展到更多数量的荧光染料，从而获得每个细胞更多的信息。研究人员将能够监测：各种表面和细胞内标记物，评估细胞离子浓度的变化（例如钙）、细胞周期分布、信号传导途径以及研究蛋白质-蛋白质和蛋白质-脂质相互作用。核心研究设施对于研究企业（例如 SDM 的研究企业）的成功至关重要，因为它们为研究人员提供了在研究活动中获得先进技术的机会，而由于财务原因，个别科学家或实验室无法负担得起这些技术。 SDM 流式细胞术设施支持超过 11 位科学家的多样化需求，他们的研究工作重点是促进口腔健康。受支持的项目特别侧重于口腔和颅面组织对生理和病理刺激的反应的研究，重点关注三个领域：炎症和免疫、传染病以及软和硬结缔组织的生物学。公共卫生相关性：SDM 流式细胞仪设施和购买最先进的流式细胞仪所需的资金对于正在进行的研究至关重要，这些研究阐明牙周病、疱疹感染、口腔癌和与口腔组织代谢相关的疾病等的致病机制。特别重要的是，该设施为目前由 NIH 资助的研究提供了必要的支持，并为我们的研究人员竞争未来的联邦研究支持以增进我们对口腔疾病发病机制的了解提供了优势。