Time-domain/ELDOR EPR Spectrometer

时域/ELDOR EPR 光谱仪

• 批准号: 7596125
• 负责人: ALEX I. SMIRNOV
• 金额: $ 50万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7596125
• 关键词: 20 year old; Applications Grants; Area; Binding; Biology; Catalytic Domain; Chemistry; Development; Drug Delivery Systems; Electronics; Enzymes; Funding; Hybrids; Ions; Label; Measures; Membrane Proteins; Metals; Methodology; Methods; North Carolina; Phospholipids; Physiologic pulse; Principal Investigator; Protein Microchips; Proteins; Public Health; RNA; Recovery; Relaxation; Research; Research Personnel; Research Project Grants; Structure; System; Time; Training; Training Programs; Transfer RNA; Universities; Virion; enzyme structure; graduate student; instrument; instrumentation; lipid transfer protein; nanoparticle; nanoscale; public health relevance; research study; self assembly

DESCRIPTION (provided by applicant): This is a Shared Instrumentation Grant proposal for the acquisition of a modern commercial CW and FT-EPR Bruker EleXsys X-band spectrometer for use by a core group of six established Principal Investigators and one beginning investigator from Universities in the Research Triangle area of North Carolina. Currently, there is no such instrument in the Triangle area. The ongoing research projects involve applications of modern time-domain EPR methods including pulsed saturation recovery and HYSCORE to a wide range of biomedical problems and systems from hybrid nanoscale assemblies for membrane protein biochips to lipid transfer proteins, self-assembly of tRNA and drug-delivery assisted by nanoparticle-templated virions, and the structures of the enzymes' catalytic sites. Currently, all these projects are either pursued with available continuous wave X-band EPR instruments that are at least 20 years old or in collaborating with other groups that have Bruker EleXsys X-band spectrometer available. With this proposed major upgrade all seven core research projects will benefit from recent developments in instrumentation and methodology of time-domain EPR methods at X-band. Specifically, we will be able to 1) obtain long-range (to up to 60-70 angstrom) distance constrains for large biomolecular assemblies and partially folded structures; 2) directly measure electronic relaxation rates of specifically labeled proteins, RNAs, and phospholipids in the presence and absence of paramagnetic relaxation agents in order to gain valuable structural information; and 3) obtain unique information on paramagnetic metal ion coordination upon substrate binding to catalytic enzymes (HYSCORE experiment).PUBLIC HEALTH RELEVANCE: The relevance of this project to public health will be in providing primarily NIH-funded investigators as well as other investigators from NCSU, UNC, and the Research Triangle area with an advanced FT- EPR spectrometer as these investigators develop new projects involving structure-function studies of proteins and RNAs. The new FT-EPR spectrometer will also enhance training of graduate students from the newly established NCSU RNA Biology/Chemistry -Biology Interface Training Program that involves ten NCSU Departments.

描述（由申请人提供）：这是一份共享仪器拨款提案，用于购买一台现代商用CW和FT-EPR布鲁克EleXsys x波段光谱仪，供北卡罗莱纳州三角研究地区大学的六名主要研究人员和一名初级研究人员组成的核心小组使用。目前，在三角地区没有这样的仪器。正在进行的研究项目涉及将现代时域EPR方法（包括脉冲饱和恢复和HYSCORE）应用于广泛的生物医学问题和系统，从膜蛋白生物芯片的混合纳米级组装到脂质转移蛋白，tRNA的自组装和纳米颗粒模板病毒粒子辅助的药物递送，以及酶催化位点的结构。目前，所有这些项目要么使用已有至少20年历史的连续波x波段EPR仪器，要么与其他拥有布鲁克EleXsys x波段光谱仪的团队合作。随着这一提议的重大升级，所有七个核心研究项目将受益于x波段时域EPR方法的仪器和方法的最新发展。具体来说，我们将能够1)获得大型生物分子组装和部分折叠结构的远程（高达60-70埃）距离限制；2)在顺磁弛豫剂存在和不存在的情况下，直接测量特异性标记的蛋白质、rna和磷脂的电子弛豫率，以获得有价值的结构信息；3)获得底物与催化酶结合时顺磁性金属离子配位的独特信息（HYSCORE实验）。公共卫生相关性：该项目与公共卫生的相关性将主要是为美国国立卫生研究院资助的研究人员以及NCSU， UNC和研究三角地区的其他研究人员提供先进的FT- EPR光谱仪，因为这些研究人员正在开发涉及蛋白质和rna结构-功能研究的新项目。新的FT-EPR光谱仪还将加强对新成立的NCSU RNA生物学/化学-生物学界面培训计划的研究生的培训，该计划涉及NCSU的十个系。

项目成果

The Hydroxyl Radical is a Critical Intermediate in the Voltammetric Detection of Hydrogen Peroxide.

• DOI: 10.1021/jacs.5b13376
• 发表时间: 2016-03-02
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Roberts JG;Voinov MA;Schmidt AC;Smirnova TI;Sombers LA
• 通讯作者: Sombers LA

Oxidation of Pyrrole by Dehaloperoxidase-Hemoglobin: Chemoenzymatic Synthesis of Pyrrolin-2-Ones.

• DOI: 10.1039/c7cy00781g
• 发表时间: 2017
• 期刊: Catalysis science & technology
• 影响因子: 5
• 作者: McCombs NL;Smirnova T;Ghiladi RA
• 通讯作者: Ghiladi RA

Surface electrostatics of lipid bilayers by EPR of a pH-sensitive spin-labeled lipid.

通过 pH 敏感的自旋标记脂质的 EPR 分析脂质双层的表面静电。

• DOI: 10.1016/j.bpj.2012.11.3806
• 发表时间: 2013
• 期刊: Biophysical journal
• 影响因子: 3.4
• 作者: Voinov,MaximA;Rivera-Rivera,Izarys;Smirnov,AlexI
• 通讯作者: Smirnov,AlexI

Enhancing sensitivity of Double Electron-Electron Resonance (DEER) by using Relaxation-Optimized Acquisition Length Distribution (RELOAD) scheme.

• DOI: 10.1016/j.jmr.2018.12.004
• 发表时间: 2019-01
• 期刊: Journal of magnetic resonance
• 影响因子: 2.2
• 作者: Sergey Milikisiyants;M. Voinov;A. Marek;M. Jafarabadi;Jing Liu;Rong Han;Shenlin Wang;A. Smirnov

Peroxygenase and oxidase activities of dehaloperoxidase-hemoglobin from Amphitrite ornata.

• DOI: 10.1021/ja500293c
• 发表时间: 2014-06-04
• 期刊: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
• 影响因子: 15
• 作者: Barrios, David A.;D'Antonio, Jennifer;McCombs, Nikolette L.;Zhao, Jing;Franzen, Stefan;Schmidt, Andreas C.;Sombers, Leslie A.;Ghiladi, Reza A.
• 通讯作者: Ghiladi, Reza A.