Purchase of a Cryo-Probe to Upgrade a 500 MHz NMR System for BioNMR Applications

购买冷冻探针来升级 500 MHz NMR 系统以用于 BioNMR 应用

• 批准号: 7591326
• 负责人: Om Prakash
• 金额: $ 31.61万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7591326
• 关键词: Alzheimer' s Disease; Amyloid beta-Protein; Arts; Ataxia; Binding; Biomedical Research; Biomolecular Nuclear Magnetic Resonance; Carbon; Chitin; Collaborations; Cystic Fibrosis; Data; Dementia; Detection; Disease; Disease Vectors; Distant; Funding; Glucans; Health; Hour; Human; Immunity; Inflammatory; Insect Proteins; Insecta; Institution; Ischemia; Kansas; Lysine; N.I.H. Research Support; Noise; Nucleocapsid Proteins; Peptides; Performance; Proteins; Protons; Research Personnel; Research Project Grants; SARS coronavirus; Sampling; Signal Transduction; Sodium Chloride; Solubility; Solutions; Structure; System; Tertiary Protein Structure; Time; United States National Institutes of Health; Universities; cryogenics; human disease; improved; instrument; instrumentation; programs; public health relevance; research study

DESCRIPTION (provided by applicant): The Kansas State University (KSU) Biomolecular NMR Facility, which has served more than 23 separate NIH-funded research projects, request funding to purchase a 5 mm triple resonance cryogenic probe with enhanced carbon sensitivity and salt tolerant proton coil for an existing Varian 500 NMR System(R) . This acquisition of a cryogenic probe will afford a 2-3 fold enhancement in sensitivity compared with conventional probes and will be of great use to eight major users and many other NIH supported investigators at this campus studying mass-limited biomolecules having less stability and/or low solubility and biomacromolecules that show concentration-dependent aggregation. Currently our users are constrained with the present instrumentation due to the limited sensitivity of the conventional probe. Many of our local researchers supported by NIH funding are forced to go to distant facilities for 2D and 3D NMR studies on their diluted samples, as our 500 MHz NMR spectrometer equipped with a conventional probe is limited in its ability to perform these experiments in a timely manner and in some cases not at all. The cryogenic probe is essential for our 500 MHz console to perform long hours 2D and 3D NMR experiments for studying solution structure and dynamics of large biomolecules and other compounds available in limited amount. The leading performance benefits of the upgraded 500 MHz NMR spectrometer with the requested cryogenic probe will be: a) improved signal-to-noise ratio in the NMR spectra, b) reduced experimental time for multi-dimensional NMR experiments, c) lowered detection limit, and d) enhanced capability to acquire NMR data on diluted samples and on biomacromolecules with low solubility and or less stability. In brief this upgrade will facilitate KSU Biomolecular NMR facility in performing multi-dimensional NMR experiments on proteins/peptides and other biomacromolecules available in low concentration with best possible sensitivity on the existing instrument. The funding of this project will provide a shared instrumentation necessary to complete on going NIH supported research programs at KSU and to elevate inter-institutional biomedical research collaborations between KSU NMR facility and nearby institutions. PUBLIC HEALTH RELEVANCE: Most of the research projects in this application aim at understanding the structure and function of a variety of protein domains relevant to human health. These include chitin binding domain and 2-1, 3 glucan binding domain of insect proteins, lysine-rich domain of the SARS- CoV nucleocapsid protein, channel forming peptides, amyloid beta peptides and biophysical mechanism of protein-protein or protein-peptide interactions, particularly in immunity and inflammatory diseases and in insects as disease vectors. The acquisition of a state-of-the-art cryogenic probe for our existing 500 MHz NMR console will significantly enhance or create new feasibility of such studies of broad importance in a number of human diseases, such as Alzheimer's dementia, ataxia, ischemia, and cystic fibrosis. .

描述（由申请人提供）：堪萨斯州立大学（KSU）生物分子核磁共振设施已为超过23个独立的NIH资助的研究项目提供服务，要求提供资金购买5 mm三重共振低温探头，该探头具有增强的碳灵敏度和耐盐质子线圈，用于现有的瓦里安500核磁共振系统（R）。与传统探针相比，这种低温探针的获得将提供2-3倍的灵敏度增强，并且将对八个主要用户和该校园的许多其他NIH支持的研究人员非常有用，这些研究人员研究具有较低稳定性和/或低溶解度的质量受限生物分子以及显示浓度依赖性聚集的生物大分子。目前，由于传统探头的灵敏度有限，我们的用户受到本仪器的限制。我们的许多当地研究人员在NIH的资助下被迫前往遥远的设施，对他们稀释的样品进行2D和3D NMR研究，因为我们配备传统探头的500 MHz NMR光谱仪在及时执行这些实验的能力方面受到限制，在某些情况下根本没有。低温探头对于我们的500 MHz控制台至关重要，可以进行长时间的2D和3D NMR实验，以研究大生物分子和其他化合物的溶液结构和动力学。升级后的500 MHz NMR光谱仪配备所需的低温探头，其主要性能优势将是：a）NMR光谱的信噪比提高，B）多维NMR实验的实验时间缩短，c）检测限降低，以及d）获得稀释样品和低溶解度和/或稳定性较差的生物大分子NMR数据的能力增强。简而言之，这一升级将促进KSU生物分子NMR设施在进行多维NMR实验的蛋白质/肽和其他生物大分子在低浓度与现有仪器的最佳灵敏度。该项目的资金将提供必要的共享仪器，以完成正在进行的NIH支持的研究计划在KSU和提升KSU NMR设施和附近机构之间的机构间生物医学研究合作。公共卫生相关性：本申请中的大多数研究项目旨在了解与人类健康相关的各种蛋白质结构域的结构和功能。这些包括昆虫蛋白质的几丁质结合结构域和2-1，3葡聚糖结合结构域、SARS-CoV核衣壳蛋白质的富含赖氨酸的结构域、通道形成肽、淀粉样β肽和蛋白质-蛋白质或蛋白质-肽相互作用的生物物理机制，特别是在免疫和炎性疾病中以及在作为疾病载体的昆虫中。为我们现有的500 MHz NMR控制台购买最先进的低温探头将显著增强或创造新的可行性，这些研究在许多人类疾病中具有广泛的重要性，如阿尔茨海默氏痴呆症，共济失调，缺血和囊性纤维化。.