Purchase of a Cryo-Probe to Upgrade a 500 MHz NMR System for BioNMR Applications

购买冷冻探针来升级 500 MHz NMR 系统以用于 BioNMR 应用

• 批准号: 7591326
• 负责人: Om Prakash
• 金额: $ 31.61万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7591326
• 关键词: Alzheimer' s Disease; Amyloid beta-Protein; Arts; Ataxia; Binding; Biomedical Research; Biomolecular Nuclear Magnetic Resonance; Carbon; Chitin; Collaborations; Cystic Fibrosis; Data; Dementia; Detection; Disease; Disease Vectors; Distant; Funding; Glucans; Health; Hour; Human; Immunity; Inflammatory; Insect Proteins; Insecta; Institution; Ischemia; Kansas; Lysine; N.I.H. Research Support; Noise; Nucleocapsid Proteins; Peptides; Performance; Proteins; Protons; Research Personnel; Research Project Grants; SARS coronavirus; Sampling; Signal Transduction; Sodium Chloride; Solubility; Solutions; Structure; System; Tertiary Protein Structure; Time; United States National Institutes of Health; Universities; cryogenics; human disease; improved; instrument; instrumentation; programs; public health relevance; research study

DESCRIPTION (provided by applicant): The Kansas State University (KSU) Biomolecular NMR Facility, which has served more than 23 separate NIH-funded research projects, request funding to purchase a 5 mm triple resonance cryogenic probe with enhanced carbon sensitivity and salt tolerant proton coil for an existing Varian 500 NMR System(R) . This acquisition of a cryogenic probe will afford a 2-3 fold enhancement in sensitivity compared with conventional probes and will be of great use to eight major users and many other NIH supported investigators at this campus studying mass-limited biomolecules having less stability and/or low solubility and biomacromolecules that show concentration-dependent aggregation. Currently our users are constrained with the present instrumentation due to the limited sensitivity of the conventional probe. Many of our local researchers supported by NIH funding are forced to go to distant facilities for 2D and 3D NMR studies on their diluted samples, as our 500 MHz NMR spectrometer equipped with a conventional probe is limited in its ability to perform these experiments in a timely manner and in some cases not at all. The cryogenic probe is essential for our 500 MHz console to perform long hours 2D and 3D NMR experiments for studying solution structure and dynamics of large biomolecules and other compounds available in limited amount. The leading performance benefits of the upgraded 500 MHz NMR spectrometer with the requested cryogenic probe will be: a) improved signal-to-noise ratio in the NMR spectra, b) reduced experimental time for multi-dimensional NMR experiments, c) lowered detection limit, and d) enhanced capability to acquire NMR data on diluted samples and on biomacromolecules with low solubility and or less stability. In brief this upgrade will facilitate KSU Biomolecular NMR facility in performing multi-dimensional NMR experiments on proteins/peptides and other biomacromolecules available in low concentration with best possible sensitivity on the existing instrument. The funding of this project will provide a shared instrumentation necessary to complete on going NIH supported research programs at KSU and to elevate inter-institutional biomedical research collaborations between KSU NMR facility and nearby institutions. PUBLIC HEALTH RELEVANCE: Most of the research projects in this application aim at understanding the structure and function of a variety of protein domains relevant to human health. These include chitin binding domain and 2-1, 3 glucan binding domain of insect proteins, lysine-rich domain of the SARS- CoV nucleocapsid protein, channel forming peptides, amyloid beta peptides and biophysical mechanism of protein-protein or protein-peptide interactions, particularly in immunity and inflammatory diseases and in insects as disease vectors. The acquisition of a state-of-the-art cryogenic probe for our existing 500 MHz NMR console will significantly enhance or create new feasibility of such studies of broad importance in a number of human diseases, such as Alzheimer's dementia, ataxia, ischemia, and cystic fibrosis. .

描述(由申请人提供)：堪萨斯州立大学(KSU)生物分子核磁共振设备已经为23个单独的NIH资助的研究项目提供了服务，该设备申请资助，为现有的瓦里安500核磁共振系统(R)购买一个5毫米三共振低温探头，该探头具有增强的碳灵敏度和耐盐质子线圈。这一低温探头的获得将使灵敏度比传统探头提高2-3倍，并将对该园区的八个主要用户和许多其他NIH支持的研究人员非常有用，他们正在研究稳定性较差和/或溶解度较低的质量受限生物分子和显示浓度依赖聚集的生物大分子。目前，由于传统探头的灵敏度有限，我们的用户受到现有仪器的限制。许多得到美国国立卫生研究院资助的当地研究人员被迫前往遥远的设施对稀释后的样品进行2D和3D核磁共振研究，因为我们配备了传统探头的500 MHz核磁共振光谱仪在及时执行这些实验方面的能力有限，在某些情况下甚至根本不能。低温探头是我们500 MHz控制台执行长时间2D和3D核磁共振实验的关键，用于研究大生物分子和其他限量化合物的溶液结构和动力学。使用所需低温探头升级的500 MHz核磁共振光谱仪的主要性能优势将是：a)提高了核磁共振谱的信噪比，b)减少了多维核磁共振实验的实验时间，c)降低了检测极限，d)增强了获取稀释样品和低溶解度或稳定性较差的生物大分子的核磁共振数据的能力。简而言之，此次升级将有助于KSU生物分子核磁共振设备在现有仪器上以最佳的灵敏度对低浓度蛋白质/多肽和其他生物大分子进行多维核磁共振实验。该项目的资金将提供必要的共享仪器，以完成KSU由NIH支持的研究项目，并提升KSU核磁共振设施与附近机构之间的机构间生物医学研究合作。公共卫生相关性：该应用中的大多数研究项目旨在了解与人类健康相关的各种蛋白质结构和功能。这些包括昆虫蛋白的几丁质结合区和2-1，3葡聚糖结合区，SARS冠状病毒核衣壳蛋白的赖氨酸富集区，通道形成肽，淀粉样β蛋白以及蛋白质-蛋白质或蛋白质-多肽相互作用的生物物理机制，特别是在免疫和炎症性疾病以及作为疾病媒介的昆虫中。为我们现有的500 MHz核磁共振控制台购买最先进的低温探头将大大增强此类研究的可行性，或为此类研究创造新的可行性，这些研究在许多人类疾病中具有广泛重要性，如阿尔茨海默氏症、共济失调、缺血和囊性纤维化。。