MICROARRAY ANALYSIS OF ATPRP3-1, AN ARABIDOPSIS MUTANT DEFECTIVE IN TIP GROWTH

ATPRP3-1（一种尖端生长缺陷的拟南芥突变体）的微阵列分析

• 批准号: 8168182
• 负责人: Mary Barbara Tierney
• 金额: $ 0.71万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168182
• 关键词: Affect; Arabidopsis; Cell Shape; Cell Wall; Cells; Communication; Complement; Complex; Computer Retrieval of Information on Scientific Projects Database; Epitopes; Extracellular Matrix; Extracellular Matrix Proteins; Family; Flowers; Funding; Gene Expression; Genes; Grant; Growth; Hair Root; Institution; Left; Metabolism; Microarray Analysis; Pathway interactions; Phenotype; Plant Roots; Plants; Research; Research Personnel; Resources; Signal Pathway; Source; Structure; Time; United States National Institutes of Health; crosslink; member; mutant

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ATPRP3 (one member of a family extracellular matrix proteins) is exclusively expressed in growing root hairs and becomes cross-linked within the cell wall as growth ceases. We have shown that an atprp3-1 null mutant displays a complex phenotype including short branched root hairs, smaller roots and leaves, an earlier flowering time and a reduced ability to compete for resources when grown with wild type plants. All of these phenotypes are complemented when an epitope-tagged wild type copy of ATPRP3 is introduced into the mutant background. In order to identify key genes and pathways whose altered expression in root hairs contribute to the atprp3 phenotypes, we will use microarray analysis to compare gene expression in root hairs of wild type, the atprp3-1 mutant and a complemented atprp3-1mutant line. We expect to identify gene expression changes associated extracellular matrix synthesis as well as changes in gene expression of signaling pathways responsible for root hair metabolism and root/shoot communication. These observations will allow us to further investigate the mechanism through which extracellular matrix structure can affect cell shape and function in a tip-growing cell.

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