DEVELOPMENT OF TOOLS TO ANALYZE PLANT SECONDARY METABOLITE CELL-CYCLE EFFECTS

开发分析植物次生代谢物细胞周期效应的工具

• 批准号: 8168099
• 负责人: Joan Swanson
• 金额: $ 1.54万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-19 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168099
• 关键词: Affect; Animals; Cell Cycle; Cell Cycle Regulation; Cell Nucleus; Cell Proliferation; Cells; Computer Retrieval of Information on Scientific Projects Database; Data; Funding; Gene Expression; Grant; Head; Institution; Ligands; Marketing; Methods; Monitor; Nuclear; Pilot Projects; Plant Growth Regulators; Plants; Regulation; Research; Research Personnel; Resources; Signal Pathway; Signal Transduction Pathway; Source; Structure; Technology; Testing; Tissues; United States National Institutes of Health; Work; interest; novel therapeutics; response; small molecule; tool development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Plant secondary metabolites provide many leads for new therapeutics that are currently on the market. However, their mechanism of action is often not understood in either plants or animals. Our preliminary data show that, in Rubus, phenolics (a class of secondary metabolites) produced in the head structure of glandular trichomes are transported to cellular nuclei in the underlying stalk tissue. Due to the nuclear localization of these phenolics and the cell proliferative response that is subsequently induced, we hypothesize that the phenolics represent a new class of plant hormones that activate developmentally-regulated signal transduction pathways. We further hypothesize that these phenolics also are able to activate mammalian signaling pathways by mimicking small molecule ligands. In order to be competitive for further funding to test this hypothesis, we will be required to collect further preliminary data; therefore, we have formulated the following specific aims to: 1) Adapt and implement methods for cell cycle analyses for our plant tissues of interest and test the utility of these methods using plant secondary metabolites that are known to affect the cell cycle in plants; 2) Continue working on plant transformation technologies to enable the expression of genes that are involved in the cell cycle whose regulation may be altered by secondary metabolite exposure. These data will provide a pilot study to develop methods that will allow us to monitor cell cycle control in Rubus plant tissues, thus allowing further studies into the regulatory mechanisms for cell proliferation in plants and to compare these mechanisms with that in animals.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 植物次生代谢产物为目前市场上的新疗法提供了许多线索。 然而，它们的作用机制在植物或动物中往往不清楚。 我们的初步数据表明，在悬钩子属植物中，腺毛头部结构中产生的酚类物质（一类次生代谢产物）被运输到下面的茎组织中的细胞核中。 由于这些酚类物质的核定位和随后诱导的细胞增殖反应，我们假设酚类物质代表一类新的植物激素，其激活发育调节的信号转导途径。 我们进一步假设，这些酚类化合物也能够通过模拟小分子配体来激活哺乳动物信号传导途径。 为了有竞争力地获得进一步的资金来检验这一假设，我们将被要求收集进一步的初步数据;因此，我们制定了以下具体目标： 1)调整和实施我们感兴趣的植物组织的细胞周期分析方法，并使用已知会影响植物细胞周期的植物次生代谢物测试这些方法的实用性; 2）继续研究植物转化技术，以使参与细胞周期的基因表达，其调控可能会被次生代谢物暴露改变。 这些数据将提供一个试点研究，以开发方法，使我们能够监测悬钩子植物组织中的细胞周期控制，从而允许进一步研究植物细胞增殖的调控机制，并将这些机制与动物进行比较。