Subcellular Localization of Neuronal Ion Channels

神经元离子通道的亚细胞定位

• 批准号: 8003686
• 负责人: DONALD B ARNOLD
• 金额: $ 35.44万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8003686
• 关键词: Actins; Adaptor Signaling Protein; Alzheimer' s Disease; Axon; Binding; Cell membrane; Clathrin Adaptors; Defect; Dendrites; Docking; Endocytosis; Exocytosis; Figs - dietary; Funding; Goals; Golgi Apparatus; Grant; Huntington Disease; Image; Imagery; Integral Membrane Protein; Ion Channel; Kinesin; Laboratories; Life; Location; MYO5A gene; Maintenance; Mediating; Membrane; Microtubules; Modeling; Molecular; Motor; Movement; Myosin ATPase; Neurons; Pathway interactions; Phase; Physiological Processes; Play; Population; Principal Investigator; Process; Property; Proteins; Research; Role; SNAP receptor; Shapes; Sorting - Cell Movement; Structure; Surface; Synapses; Transcription Factor AP-1; Transport Vesicles; Vesicle; base; clinically significant; fluorophore; myosin VI; nervous system disorder; novel; programs; protein distribution; public health relevance; research study; syntaxin 4; trafficking

DESCRIPTION (provided by applicant): The purpose of the studies proposed in this grant is to understand the molecular mechanisms underlying polarized targeting of neuronal transmembrane proteins. The specific aims of this grant will concentrate on three main levels of polarized targeting: 1. Targeting of proteins at the plasma membrane through compartment-specific endocytosis and vesicle docking. 2. Trafficking of vesicles containing either axonal or dendritic proteins to the appropriate polarized compartment. 3. Sorting of axonal and dendritic proteins into separate vesicles. The ultimate goal of this research is to understand how these targeting mechanisms produce surface distributions of proteins that are highly polarized. Relevance Protein targeting plays an important role in many physiological processes such as the establishment and maintenance of synaptic connections. In addition, defects in trafficking are associated with numerous neurological diseases of major clinical significance such as Alzheimer's and Huntington's disease. PUBLIC HEALTH RELEVANCE: In neurons many transmembrane proteins are targeted specifically to either the axonal or dendritic compartment. The goal of this grant is to understand the molecular mechanisms that mediate this targeting.

描述(由申请者提供)：这项研究的目的是了解神经元跨膜蛋白极化靶向的分子机制。这笔赠款的具体目标将集中在三个主要水平的极化靶向：1.通过隔室特定的内吞作用和囊泡对接将蛋白质靶向质膜。2.将含有轴突或树突状蛋白的囊泡运输到适当的极化隔室。3.将轴突和树突蛋白分选成不同的小泡。这项研究的最终目标是了解这些靶向机制如何产生高度极化的蛋白质的表面分布。相关蛋白靶向在突触连接的建立和维持等许多生理过程中发挥着重要作用。此外，贩运缺陷与许多具有重大临床意义的神经系统疾病有关，如阿尔茨海默氏症和亨廷顿病。 与公共健康相关：在神经元中，许多跨膜蛋白专门针对轴突或树突室。这笔赠款的目标是了解调节这种靶向的分子机制。