Regulation of APETALA2 in flower development in Arabidopsis

APETALA2 在拟南芥花发育中的调控

• 批准号: 7898338
• 负责人: Xuemei Chen
• 金额: $ 20.47万
• 依托单位: UNIVERSITY OF CALIFORNIA RIVERSIDE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-13 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7898338
• 关键词: Address; Animals; Arabidopsis; Biology; Burn injury; Cells; Complex; Development; Developmental Process; Family; Flowers; Funding; Gene Expression Regulation; Gene Targeting; Genes; Genetic; Genetic Screening; Goals; Homeobox Genes; Knowledge; Lead; Mediating; Meristem; Messenger RNA; MicroRNAs; Modeling; Molecular; Molecular Genetics; Mouse-ear Cress; Mustard; Names; Organ; Organism; Pathway interactions; Pattern; Plants; Play; Primordium; Proteins; RNA; Regulation; Repression; Research; Research Personnel; Role; Small RNA; Specific qualifier value; Therapeutic Agents; Time; Transcriptional Regulation; Translational Repression; Undifferentiated; United States National Institutes of Health; Work; cell fate specification; genetic analysis; human disease; insight; interest; member; plant APETALA2 protein; reproductive; tool; transcription factor

We use flower development in Arabidopsis as a model to understand how cells in multicellular organisms assume their developmental fates and form distinct patterns. Our long-term goal is to identify and analyze the genetic networks and the underlying molecular mechanisms that lead to cell fate specification in the floral primordium. For a long time, three classes of floral homeotic transcription factors known as the A, B, and C genes have been the only players in floral organ identity specification. Our studies have identified a microRNA, miR172, as a translational represser of the class A gene APETALA2, indicating that posttranscriptional regulation also plays a role in flower development. In the proposed project, we will employ molecular genetic approaches to determine how miR172 fits in the regulatory circuitry governing flower development and to probe how miR172 regulates APETALA2 mRNA at the translational level. Homeotic genes that encode transcription factors act in cell fate specification in both animals and plants. Emerging evidence of microRNAs as regulators of homeotic genes in both animals and plants adds a new layer of regulation to the known transcriptional networks governing cell fate specification. The proposed research will undoubtedly provide insights into the integration of posttranscriptional and transcriptional mechanisms in developmental processes in multicellular organisms. The proposed research will also provide insights into the mechanism of microRNA-mediated translational repression of target mRNAs. Given the conserved actions of small RNAs in gene regulation in plants and animals and the potential of using small RNAs as therapeutic agents, this research will contribute to our understanding of small RNA biology in general and impact the uses of small RNAs to treat human diseases.

我们使用拟南芥花发育作为模型来了解多细胞中的细胞如何 生物体承担其发育命运并形成独特的模式。我们的长期目标是 识别和分析导致的遗传网络和潜在的分子机制 花原基中的细胞命运规范。 长期以来，三类花同源异型转录因子被称为 A、B 和 C 基因是花器官特征规范的唯一参与者。我们的研究已经确定了 microRNA，miR172，作为 A 类基因 APETALA2 的翻译抑制子，表明 转录后调控在花的发育中也发挥着作用。在拟议的项目中，我们 将采用分子遗传学方法来确定 miR172 如何适应调节电路 调控花的发育并探讨 miR172 如何在 APETALA2 mRNA 上调节 翻译水平。 编码转录因子的同源基因在两种动物的细胞命运规范中发挥作用 和植物。新的证据表明 microRNA 作为两种动物同源异型基因的调节剂 植物为已知的控制细胞命运的转录网络添加了新的调控层 规格。拟议的研究无疑将为整合提供见解 多细胞发育过程中的转录后和转录机制 有机体。拟议的研究还将提供对机制的见解 microRNA 介导的靶 mRNA 翻译抑制。鉴于保守的行动 小RNA在植物和动物基因调控中的作用以及使用小RNA作为基因调控的潜力 治疗剂，这项研究将有助于我们对小 RNA 生物学的理解 并影响小 RNA 治疗人类疾病的用途。