Development genetics of adaptive wing pattern variation in butterflies
蝴蝶适应性翼型变异的发育遗传学
基本信息
- 批准号:7982540
- 负责人:
- 金额:$ 4.76万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-07-13 至 2012-07-12
- 项目状态:已结题
- 来源:
- 关键词:AddressAntibodiesArchitectureBiological ModelsButterfliesCandidate Disease GeneCell MaturationColorComparative StudyComplexDNADataDevelopmentDiagnosisDiseaseDisease ResistanceExhibitsFigs - dietaryGene ExpressionGenesGeneticGenomicsGoalsIn Situ HybridizationIndividualModificationMutationNaturePathway interactionsPatternPhenotypePigmentation physiologic functionPigmentsPopulationProxyRNA analysisRaceRadiationRegulationRegulator GenesResearchResourcesRoleStagingSwitch GenesSystemTechniquesTissuesTranscriptVariantWingbasecolor processingdevelopmental geneticsgene interactioninsightinterestmimeticsmimicryprotein expressionpublic health relevancetrait
项目摘要
DESCRIPTION (provided by applicant): Comparative studies addressing the variation in how complex adaptive traits can be genetically regulated provide basic insights into the diverse diagnosis and treatments that may be needed to effectively target disease. Heliconius butterfly species H. erato and H. melpomene exhibit parallel color pattern diversifications across the Neotropics, forming up to 30 mimicry complexes. This highly tractable system provides numerous examples of color pattern convergence and divergence, and thus serves as a proxy for understanding the genetic regulation of rapidly evolving complex phenotypes. The genetic switches responsible for the diverse color patterns in these species have been narrowed to three loci, two of which have been refined to a genomic interval of ~400KB each. The proposed study seeks to hone in on the genes responsible for both divergent and convergent color pattern phenotypes in these butterflies. Towards this goal, microarrays containing both DNA tiled across these loci and whole-genomic transcripts will be hybridized to wing tissues across six developmental stages from five color pattern races of H. erato. Analyses of differential hybridization on these arrays will be used to assess the genes responsible for color pattern differences within each locus, the gene modules and hypothetical pathways elicited by these regulatory genes, and the genetic interactions between these pathways. Rapidly evolving traits may have high variance or even non-functionality in their underlying gene expression. Tissues of multiple individuals of each color pattern race will be hybridized to microarrays to explore the natural variation in gene expression across candidate genes. Genes implicated in color pattern will be analyzed further using spatial analysis of RNA and protein expression in wing tissue using in situ hybridization and antibodies.
Public Health Relevance: By examining the genetics behind diverse mimetic color patterns of butterflies we gain a model system for understanding how genetic interactions and gene architecture govern changes in complex traits with a high impact on survival. This will enhance understanding of the genetic regulation of the complex adaptive traits of disease and disease resistance and, therefore, highlight the multitude of strategies that may be needed to both diagnose and treat disease.
描述(由申请人提供):针对复杂适应性性状如何进行遗传调控的差异的比较研究为有效靶向疾病可能需要的多种诊断和治疗提供了基本见解。蝴蝶属Heliconius butterfly species H. erato和H.在整个新热带区,melpomene表现出平行的颜色图案多样化,形成多达30个拟态复合物。这个高度易处理的系统提供了许多颜色模式会聚和发散的例子,因此可以作为理解快速进化的复杂表型的遗传调控的代理。在这些物种中负责不同颜色模式的遗传开关已经缩小到三个位点,其中两个已经被细化到每个基因组间隔约400 KB。这项拟议中的研究旨在研究这些蝴蝶中负责发散和收敛颜色模式表型的基因。为了实现这一目标,微阵列包含DNA平铺在这些基因座和全基因组转录将杂交到翅膀组织跨越六个发育阶段,从五个颜色模式的种族H。埃拉托。这些阵列上的差异杂交分析将用于评估每个基因座内负责颜色模式差异的基因,这些调控基因引起的基因模块和假设途径,以及这些途径之间的遗传相互作用。快速进化的性状可能在其潜在的基因表达中具有高变异性或甚至无功能性。每个颜色模式种族的多个个体的组织将与微阵列杂交,以探索候选基因之间基因表达的自然变异。涉及颜色模式的基因将进一步分析使用RNA和蛋白质表达的空间分析,在翅膀组织中使用原位杂交和抗体。
公共卫生相关性:通过研究蝴蝶不同的模仿颜色模式背后的遗传学,我们获得了一个模型系统,用于了解遗传相互作用和基因结构如何控制复杂性状的变化,对生存产生很大影响。这将增强对疾病和抗病性的复杂适应性特征的遗传调控的理解,因此,突出了诊断和治疗疾病可能需要的多种策略。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Heather Marie Hines其他文献
Heather Marie Hines的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Heather Marie Hines', 18)}}的其他基金
Development genetics of adaptive wing pattern variation in butterflies
蝴蝶适应性翼型变异的发育遗传学
- 批准号:
8088133 - 财政年份:2009
- 资助金额:
$ 4.76万 - 项目类别:
Development genetics of adaptive wing pattern variation in butterflies
蝴蝶适应性翼型变异的发育遗传学
- 批准号:
7751417 - 财政年份:2009
- 资助金额:
$ 4.76万 - 项目类别:
相似海外基金
University of Aberdeen and Vertebrate Antibodies Limited KTP 23_24 R1
阿伯丁大学和脊椎动物抗体有限公司 KTP 23_24 R1
- 批准号:
10073243 - 财政年份:2024
- 资助金额:
$ 4.76万 - 项目类别:
Knowledge Transfer Partnership
Role of Natural Antibodies and B1 cells in Fibroproliferative Lung Disease
天然抗体和 B1 细胞在纤维增生性肺病中的作用
- 批准号:
10752129 - 财政年份:2024
- 资助金额:
$ 4.76万 - 项目类别:
CAREER: Next-generation protease inhibitor discovery with chemically diversified antibodies
职业:利用化学多样化的抗体发现下一代蛋白酶抑制剂
- 批准号:
2339201 - 财政年份:2024
- 资助金额:
$ 4.76万 - 项目类别:
Continuing Grant
Isolation and characterisation of monoclonal antibodies for the treatment or prevention of antibiotic resistant Acinetobacter baumannii infections
用于治疗或预防抗生素耐药鲍曼不动杆菌感染的单克隆抗体的分离和表征
- 批准号:
MR/Y008693/1 - 财政年份:2024
- 资助金额:
$ 4.76万 - 项目类别:
Research Grant
Developing first-in-class aggregation-specific antibodies for a severe genetic neurological disease
开发针对严重遗传神经系统疾病的一流聚集特异性抗体
- 批准号:
10076445 - 财政年份:2023
- 资助金额:
$ 4.76万 - 项目类别:
Grant for R&D
Discovery of novel nodal antibodies in the central nervous system demyelinating diseases and elucidation of the mechanisms through an optic nerve demyelination model
发现中枢神经系统脱髓鞘疾病中的新型节点抗体并通过视神经脱髓鞘模型阐明其机制
- 批准号:
23K14783 - 财政年份:2023
- 资助金额:
$ 4.76万 - 项目类别:
Grant-in-Aid for Early-Career Scientists
Elucidation of the mechanisms controlling the physicochemical properties and functions of supercharged antibodies and development of their applications
阐明控制超电荷抗体的理化性质和功能的机制及其应用开发
- 批准号:
23KJ0394 - 财政年份:2023
- 资助金额:
$ 4.76万 - 项目类别:
Grant-in-Aid for JSPS Fellows
Role of antibodies in hepatitis E virus infection
抗体在戊型肝炎病毒感染中的作用
- 批准号:
10639161 - 财政年份:2023
- 资助金额:
$ 4.76万 - 项目类别:
Defining the protective or pathologic role of antibodies in Post-Ebola Syndrome
定义抗体在埃博拉后综合症中的保护或病理作用
- 批准号:
10752441 - 财政年份:2023
- 资助金额:
$ 4.76万 - 项目类别:
Human CMV monoclonal antibodies as therapeutics to inhibit virus infection and dissemination
人 CMV 单克隆抗体作为抑制病毒感染和传播的治疗药物
- 批准号:
10867639 - 财政年份:2023
- 资助金额:
$ 4.76万 - 项目类别: