Mechanisms of PEN protein focal accumulation and regulation in plant immunity.

PEN蛋白在植物免疫中的聚集积累和调节机制。

基本信息

批准号：
7878550
负责人：
William R. Underwood
金额：
$ 2.61万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-07-01 至 2010-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7878550
关键词：
ATP-Binding Cassette Transporters Address Aftercare Amino Acids Antifungal Agents Arabidopsis Binding Biological Biological Phenomena Cell membrane Cell physiology Cell surface Cells Cholesterol Cystic Fibrosis Cytoskeleton Detergents Epithelial Cells Eukaryota Flagellin Future HIV Host resistance Human Immune response Immunity Infection Invaded Investigation Lipids Location Lung Membrane Membrane Microdomains Membrane Proteins Molecular Mouse-ear Cress Parasites Pattern Penetration Peptides Phosphorylation Plant Model Plants Plasmodium falciparum Play Post-Translational Protein Processing Process Proteins Pseudomonas aeruginosa Recruitment Activity Regulation Reporting Research Research Proposals Resistance Resistance to infection Role Site Study models Tertiary Protein Structure Vesicle Yeasts comparative fungus improved inhibitor/antagonist mildew novel pathogen receptor research study response syntaxin trafficking yeast two hybrid system

项目摘要

DESCRIPTION (provided by applicant): Cell polarization and focal accumulation of specific proteins to distinct membrane domains are important processes in the response of eukaryotes to attempted pathogen infection. These processes contribute to resistance to infection and are sometimes exploited by successful pathogens to gain entry into host cells. In humans, processes of cell polarization and focal protein accumulation play a role in the response to numerous pathogens including Pseudomonas aeruginosa, HIV, and the malarial parasite Plasmodium falciparum. Currently, the mechanisms underlying the recruitment of defense-related proteins to distinct locations on the cell surface in response to attempted pathogen infection are not well understood. In the model plant Arabidopsis thaliana, resistance to penetration and infection by the powdery mildew fungus Blumeria graminus f. sp. hordei (Bgh) is characterized by the focal accumulation of defense proteins at sites of attempted fungal penetration. Both PEN1, a syntaxin, and PENS, an ABC transporter, contribute to penetration resistance in Arabidopsis and are recruited to sites of interaction with the invading fungus. However, the mechanisms underlying the focal accumulation of these components of penetration resistance to sites of attempted fungal penetration are not known. Resistance of Arabidopsis to penetration by Bgh provides a novel model for studying the cell biological mechanisms underlying cell polarization and protein focal accumulation at sites of attempted pathogen infection. The specific aims of this research proposal are to: 1) Investigate the mechanisms underlying focal accumulation of the Arabidopsis PEN1 and PENS proteins at sites of attempted fungal penetration; and 2) Determine protein domains and posttranslational modifications important for the localization and defense function of the PENS ABC transporter. The proposed research will address the roles of roles of the cytoskeleton, vesicle trafficking, and lipid rafts in the focal accumulation of proteins at sites of attempted pathogen infection. Additionally, this research will address the role of elicitor-induced phosphorylation of an ABC transporter in the regulation of its activity and localization. Successful completion of these aims will improve our understanding of the mechanisms underlying cell polarization and protein focal accumulation during attempted pathogen infection.

描述（由申请人提供）：特定蛋白质对不同膜结构域的细胞极化和局灶性积累是真核生物对试图病原体感染的反应的重要过程。这些过程有助于对感染的抗性，有时成功的病原体可以利用进入宿主细胞。在人类中，细胞极化和局灶性蛋白积累的过程在对包括铜绿假单胞菌，HIV和疟疾寄生虫恶性疟原虫的众多病原体的反应中起作用。当前，尚不清楚将与防御相关的蛋白募集到细胞表面上不同位置的基础机制响应于试图的病原体感染。在模型植物拟南芥中，白粉病真菌graminus f。 sp。 Hordei（BGH）的特征是防御蛋白在未尝试的真菌渗透部位的局灶性积累。 PEN1，一种语法素和笔（ABC转运蛋白）都在拟南芥中有助于渗透性，并被招募到与入侵的真菌相互作用的部位。然而，尚不清楚这些成分的渗透耐药性分量的焦点积累的基础机制尚不清楚。拟南芥对BGH渗透的耐药性为研究细胞极化和蛋白质局灶性积累的细胞生物学机制提供了一种新型模型，并在未遂病原体感染部位的蛋白质局灶性积累。该研究建议的具体目的是：1）研究拟南芥PEN1和钢笔蛋白在未尝试的真菌渗透部位的局灶性局灶性积累的机制； 2）确定蛋白质结构域和翻译后修饰对于PENS ABC转运蛋白的定位和防御功能很重要。拟议的研究将探讨细胞骨架，囊泡运输和脂质筏在蛋白质的局灶性积累中的作用。此外，这项研究将探讨启发剂引起的ABC转运蛋白在调节其活性和定位中的磷酸化的作用。这些目标的成功完成将提高我们对试图病原体感染过程中细胞极化和蛋白质局灶性积累的机制的理解。