Identification of Uncultivated and Unrecognized Pathogens of Periodontitis

未培养和未被识别的牙周炎病原体的鉴定

基本信息

  • 批准号:
    7893783
  • 负责人:
  • 金额:
    $ 70.07万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2007
  • 资助国家:
    美国
  • 起止时间:
    2007-08-01 至 2011-07-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): In recent years, studies using molecular techniques have indicated that as many as 700 taxa may be detected in the oral cavity, about 300 or more in subgingival plaque, most of which were uncultivable or at least not recognized. It is likely that some of these uncultivable taxa may be periodontal pathogens. Thus, Specific Aim 1 will test the hypothesis that cultivated or uncultivated bacterial species, not yet recognized as pathogens, are associated with periodontitis. In a cross-sectional study, subgingival biofilm samples, taken from the mesial aspect of up to 28 teeth in each of 80 periodontally healthy and 120 periodontitis subjects. Each sample will be split and individually examined using two microbiological assessments. The first method, direct checkerboard hybridization using whole genomic DNA probes to 40 species, will provide positive and negative controls for known species that discriminate periodontitis from health. Extensive experience indicates that species such as P. gingivalis and T. forsythia will differ significantly from health to disease while most remaining species will not. The second microarray chip assay will detect the presence of 300 taxa; i.e. essentially all of the frequently detected cultivable and as yet uncultivated subgingival taxa. The second major criterion to distinguish periodontal pathogens from non pathogens is based on the notion that suppression of a pathogen should lead to clinical improvement, while failure to reduce that species would lead to disease progression. This criterion will be utilized in Specific Aim 2 which will examine the effects of periodontal therapy on newly recognized potential periodontal pathogens and clinical parameters. The 120 periodontitis subjects from Specific Aim 1 will be treated by SRP and 2 weeks of systemically administered metronidazole and amoxicillin. Clinical monitoring and microbiological sampling will be repeated 3 months post therapy and the subgingival plaque samples examined by the two microbiological methods as described in SA1. Associations will be sought between changes in levels of each test taxon and clinical response to therapy. Since periodontal diseases are infections, it would be difficult to suggest studies that are more important than defining the causative agent(s) of the diseases. This study will examine the association of the uncultivated group of species to periodontal health and disease, thus taking the critical first steps in discriminating the pathogenic from commensal organisms. The identification of such pathogens should, over time, lead to better diagnosis of disease, optimal treatment strategies for the individual patient, new therapeutic approaches and carefully targeted approaches to prevention.
描述(由申请人提供):近年来,使用分子技术的研究表明,口腔中可检测到多达700个分类群,龈下菌斑中约300个或更多,其中大多数是不可培养的或至少未被识别。这些不可培养的类群中的一些可能是牙周病原体。因此,具体目标1将检验尚未被认为是病原体的培养或未培养的细菌物种与牙周炎相关的假设。在一项横断面研究中,龈下生物膜样本,取自80名牙周健康和120名牙周炎受试者中各28颗牙齿的近中面。将每份样本分开,并使用两次微生物评估进行单独检查。第一种方法,直接棋盘杂交使用全基因组DNA探针的40个物种,将提供阳性和阴性对照的已知物种,区分牙周炎的健康。广泛的经验表明,物种,如牙龈卟啉单胞菌和T。健康与疾病之间的差异很大,而大多数剩余的物种则不会。第二微阵列芯片测定将检测300个分类群的存在;即基本上所有频繁检测的可培养的和尚未培养的龈下分类群。区分牙周病原体与非病原体的第二个主要标准是基于这样的概念:抑制病原体应该导致临床改善,而未能减少该物种将导致疾病进展。这一标准将用于具体目标2,该目标2将检查牙周治疗对新识别的潜在牙周病原体和临床参数的影响。来自特定目标1的120名牙周炎受试者将通过SRP和2周的甲硝唑和阿莫西林全身给药进行治疗。治疗后3个月将重复进行临床监测和微生物采样,并按照SA 1中所述的两种微生物方法检查龈下菌斑样本。将寻求每个测试分类群的水平变化与对治疗的临床反应之间的关联。由于牙周病是一种感染,因此很难提出比确定疾病的病原体更重要的研究。这项研究将探讨未种植的物种组与牙周健康和疾病的关系,从而采取关键的第一步,从牙周组织中区分病原体。随着时间的推移,查明这些病原体应能更好地诊断疾病,为个别病人制定最佳治疗战略,采取新的治疗办法,并采取有针对性的预防办法。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Lessons learned and unlearned in periodontal microbiology.
  • DOI:
    10.1111/prd.12010
  • 发表时间:
    2013-06
  • 期刊:
  • 影响因子:
    18.6
  • 作者:
    Teles R;Teles F;Frias-Lopez J;Paster B;Haffajee A
  • 通讯作者:
    Haffajee A
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RICARDO P TELES其他文献

RICARDO P TELES的其他文献

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{{ truncateString('RICARDO P TELES', 18)}}的其他基金

Biomarkers of Periodontal Disease Progression
牙周病进展的生物标志物
  • 批准号:
    8501416
  • 财政年份:
    2010
  • 资助金额:
    $ 70.07万
  • 项目类别:
Biomarkers of Periodontal Disease Progression
牙周病进展的生物标志物
  • 批准号:
    7952313
  • 财政年份:
    2010
  • 资助金额:
    $ 70.07万
  • 项目类别:
Biomarkers of Periodontal Disease Progression
牙周病进展的生物标志物
  • 批准号:
    8305414
  • 财政年份:
    2010
  • 资助金额:
    $ 70.07万
  • 项目类别:
Biomarkers of Periodontal Disease Progression
牙周病进展的生物标志物
  • 批准号:
    9282114
  • 财政年份:
    2010
  • 资助金额:
    $ 70.07万
  • 项目类别:
Biomarkers of Periodontal Disease Progression
牙周病进展的生物标志物
  • 批准号:
    8217313
  • 财政年份:
    2010
  • 资助金额:
    $ 70.07万
  • 项目类别:
Biomarkers of Periodontal Disease Progression
牙周病进展的生物标志物
  • 批准号:
    8914088
  • 财政年份:
    2010
  • 资助金额:
    $ 70.07万
  • 项目类别:
DEVELOPMENT OF A CHECKERBOARD IMMUNOBLOT
棋盘免疫印迹的开发
  • 批准号:
    7718984
  • 财政年份:
    2008
  • 资助金额:
    $ 70.07万
  • 项目类别:
DEVELOPMENT OF A CHECKERBOARD IMMUNOBLOT
棋盘免疫印迹的开发
  • 批准号:
    7607014
  • 财政年份:
    2007
  • 资助金额:
    $ 70.07万
  • 项目类别:
Quantification of Host Markers in GCF Using CBIB
使用 CBIB 对 GCF 中的宿主标记进行量化
  • 批准号:
    7257894
  • 财政年份:
    2006
  • 资助金额:
    $ 70.07万
  • 项目类别:
Quantification of Host Markers in GCF Using CBIB
使用 CBIB 对 GCF 中的宿主标记进行量化
  • 批准号:
    7036069
  • 财政年份:
    2006
  • 资助金额:
    $ 70.07万
  • 项目类别:

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任务 C19:阿莫西林和阿莫西林/克拉维酸在 NHP 炭疽模型中的功效
  • 批准号:
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    9430036
  • 财政年份:
    2016
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阿莫西林与安慰剂治疗巴基斯坦 2-59 个月儿童非严重肺炎的双盲随机社区试验
  • 批准号:
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  • 批准号:
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  • 批准号:
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