PROTEOMIC METHOD FOR IDENTIFYING MEMBRANE PROTEINS

鉴定膜蛋白的蛋白质组学方法

• 批准号: 7955062
• 负责人: MARK S LADINSKY
• 金额: $ 1.07万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7955062
• 关键词: Cell physiology; Cellular Structures; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; Integral Membrane Protein; Journals; Membrane; Membrane Proteins; Methods; Nature; Pharmacologic Substance; Preparation; Procedures; Proteome; Proteomics; Research; Research Personnel; Resources; Shotguns; Source; Staging; Transmembrane Domain; United States National Institutes of Health; Work; electron tomography; high throughput analysis; reconstruction; three dimensional structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Integral membrane proteins perform crucial cellular functions and are the targets for the majority of pharmaceutical agents. However, the hydrophobic nature of their membrane-embedded domains makes them difficult to work with. Here, we describe a shotgun proteomic method for the high-throughput analysis of the membrane-embedded transmembrane domains of integral membrane proteins which extends the depth of coverage of the membrane proteome. Electron tomography and 3D reconstruction is used to deduce the 3D structure of the membrane preparations at different stages of the procedures. This work is currently in press in the Journal of Proteome Research.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 整合膜蛋白执行关键的细胞功能，并且是大多数药剂的靶标。 然而，它们的膜嵌入结构域的疏水性使得它们难以工作。 在这里，我们描述了一种鸟枪蛋白质组学方法的膜嵌入的跨膜结构域的整合膜蛋白，扩展膜蛋白质组的覆盖深度的高通量分析。 电子断层扫描和3D重建被用来推断在程序的不同阶段的膜制剂的3D结构。 这项工作目前正在出版的蛋白质组研究杂志。