SYSTEMS ANALY OF PKA-MEDIATED PHOSPH GRADIENTS IN LIVE CARDIAC MYOCYTES

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 cAMP信号传导的区室化和动力学是cAMP无数细胞作用中特异性的重要决定因素。然而，缺乏对cAMP和PKA信号的时空控制机制的定量理解。我们建立了活细胞FRET成像和机械计算建模的集成作为一个系统的方法，细胞内信号网络的定量表征有价值。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Compartmentation and dynamics of cAMP signaling are important determinants of specificity among cAMP's myriad cellular roles. However, a quantitative understanding of mechanisms underlying spatiotemporal control of cAMP and PKA signaling is lacking. We established the integration of live-cell FRET imaging and mechanistic computational modeling as a systems approach valuable for quantitative characterization of intracellular signaling networks.