SYSTEMS ANALY OF PKA-MEDIATED PHOSPH GRADIENTS IN LIVE CARDIAC MYOCYTES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在cAMP的众多细胞作用中，cAMP信号的区隔和动力学是决定其特异性的重要因素。然而，对cAMP和PKA信号的时空调控机制缺乏定量的了解。我们建立了活细胞FRET成像和机械计算建模的集成，作为一种对定量描述细胞内信号网络有价值的系统方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Compartmentation and dynamics of cAMP signaling are important determinants of specificity among cAMP's myriad cellular roles. However, a quantitative understanding of mechanisms underlying spatiotemporal control of cAMP and PKA signaling is lacking. We established the integration of live-cell FRET imaging and mechanistic computational modeling as a systems approach valuable for quantitative characterization of intracellular signaling networks.