Mosaic Analysis with Double Markers in Mice

描述（由申请人提供）： 遗传镶嵌是指不同基因型的体细胞存在于同一动物体内，已被广泛用于研究多细胞生物的生物学过程。 通过在期望的时间敲除确定的细胞群体中的感兴趣的候选基因，人们可以研究感兴趣的生物过程中的基因功能，同时绕过其他组织或早期发育阶段对基因的可能要求。 如果候选基因仅在限定细胞类型的小群体中被去除，则还可以分析基因功能的细胞自主性。 此外，镶嵌分析可用于创建由体细胞突变引起的人类疾病的动物模型。 我们最近在小鼠中开发了一种称为“MADM”（用于双标记的镶嵌分析）的遗传镶嵌系统，该系统允许同时对定义的神经元群体进行体内标记和遗传操作，直至单个分离神经元的水平。 我们已经在小鼠6号染色体的ROSA 26基因座处建立了MADM，以表明：1）Cre-loxP系统可以有效地发生染色体间重组; 2）MADM可以用于在标记细胞的小群体中创建条件性敲除以研究基因功能; 3）MADM可以用于研究神经元谱系和布线模式之间的关系。 我们也有初步的数据，MADM可以扩大到其他染色体使用有针对性的敲入和随机ES细胞转基因方法。 我们现在建议将MADM系统扩展到所有小鼠染色体，以便可以对小鼠中的绝大多数基因进行基于MADM的镶嵌分析。 我们还建议使用MADM所提供的功能，以研究神经元活动在单个神经元形态成熟和电路发展中的作用。 最后，我们将应用MADM来研究与人类神经系统疾病有关的几个基因，包括无脑畸形和自闭症谱系障碍。 公共卫生相关性：我们建议扩展我们开发的一种技术，该技术允许在小鼠中的一小群明确定义的细胞中破坏基因。 利用这项技术，我们将研究神经元活动形式的经验如何塑造神经元发育和大脑布线，以及为什么某些基因在人类中突变时会导致毁灭性的神经问题。 这些研究将对我们理解无脑畸形和Smith-Magenis综合征的发病机制产生直接影响。

DESCRIPTION (provided by applicant): Genetic mosaics, in which somatic cells of different genotypes reside in the same animal, have been widely used to study biological processes in multicellular organisms. By knocking out a candidate gene of interest in a defined population of cells at a desirable time, one can study gene function in biological processes of interest while bypassing possible requirements for the gene in other tissues or at earlier developmental stages. One can also analyze the cell autonomy of gene function if the candidate gene is removed only in small populations of defined cell types. In addition, mosaic analysis can be used to create animal models for human diseases that result from somatic mutations. We have recently developed a genetic mosaic system in mice termed "MADM" (for Mosaic Analysis with Double Markers), which allows simultaneous in vivo labeling and genetic manipulation of defined neuronal populations, down to the level of single isolated neurons. We have established MADM at the ROSA26 locus of mouse chromosome 6 to show that 1) inter-chromosomal recombination can occur efficiently the Cre-loxP system; 2) MADM can be used to create conditional knockouts in small populations of labeled cells to study gene function; 3) MADM can be used to investigate the relationship between neuronal lineage and wiring patterns. We have also preliminary data that MADM can be expanded to other chromosomes using targeted knockin and random ES cell transgenesis approaches. We now propose to expand the MADM system to all mouse chromosomes so that one can perform MADM-based mosaic analysis for vast majority of genes in the mouse. We also propose to use the features afforded by MADM to investigate the role of neuronal activity in morphological maturation of individual neurons and in circuit development. Lastly, we will apply MADM to study several genes implicated in human neurological diseases, including lissencephaly and autism- spectrum disorders. PUBLIC HEALTH RELEVANCE: We propose to expand a technique we developed that allows one to disrupt a gene in small population of well-defined cells in the mouse. Utilizing this technique, we will study how experience in the form of neuronal activity shapes neuronal development and brain wiring, and why certain genes, when mutated in human, cause devastating neurological problems. These studies will have direct implications to our understanding of the pathogenesis of lissencephaly and Smith-Magenis Syndrome.