Adminstrative Core

组织学、细胞和动脉粥样硬化核心将为该计划内的研究人员提供五项主要服务 项目。1)组织处理和免疫-组织分析，2)照片-文档，图像 分析和量化终点数据并对其进行显著性统计检验，3)小鼠脂肪组学 分析，4)原代内皮细胞分离培训和质量控制的纯度和5)分离和 骨髓来源的巨噬细胞培养和试剂。核心拥有丰富的经验和 将与克利夫兰诊所的联席主任乔纳森·史密斯博士协调详细和定量的 这些研究需要对动脉粥样硬化病变和小鼠脂质组学进行分析。高级组织学 处理和免疫组织化学将适用于所有项目。该计划目标中的一个关键问题是 主要是利用原代细胞群，主要是内皮细胞和骨髓来源的巨噬细胞。 由于不同的实验室和个人目前正在以不同的效率和水平分离原代细胞 应用于群体纯度的质量控制评估，我们将原代细胞分离和 分析进入核心。来自核心的支持将允许研究人员开发项目和实验 没有技术困难的定量或细胞分离和分析方案是不可或缺的 计划项目的组成部分，为每个项目提供扩展的机会和目标，并将 继续成为未来努力不可或缺的资源。 相关性(请参阅说明)： 核心融资机制对于支持个别项目的发展是必要的。专门性的应用 统一的技术和模型将改进对结果的解释，交叉解释 并提供有关动脉粥样硬化机制的最相关信息 以及血管疾病中的炎症事件。

The Histology, Cell and Atheroma Core will provide five main services for investigators within the program project. 1) Tissue Processing and Immuno- and Histological Analysis, 2) Photo-documentation, image analysis and quantification and statistical testing of endpoint data for significance, 3) Mouse Lipidomic Analysis, 4) Primary endothelial cell isolation training and quality control for purity and 5) Isolation and culturing of bone marrow-derived macrophage training and reagents. The core has abundant experience and will coordinate with co-director, Dr. Jonathan Smith, Cleveland Clinic, on the detailed and quantitative analysis of atheroma lesions and murine lipidomics needed for these studies. Advanced histological processing and immunohistochemistry will be available to all projects. A key issue in the program goals has been the utilization of primary cell populations, primarily endothelial and bone marrow derived macrophage. Since different labs and individuals are currently isolating primary cells with variable levels of efficiency and application of quality control assessment of population purity, we have incorporated primary cell isolation and analysis into the core. The support from the core will allow investigators to develop projects and experiments without having technical difficulties with quantitative or cell isolation and analysis protocols is an integral component of the program project, provides expanded opportunities and goals for each project and will continue to be an indispensible resource for future efforts. RELEVANCE (See instructions): The Core Facility is necessary to support development of the individual projects. The application of specific techniques and models in a uniform manner will improve the interpretation of results, cross interpretation between projects and provide the most relevant information regarding the mechanisms of atherosclerosis and inflammatory events in vascular disease.