Tissue

组织核心为执行肺结构提供专业知识、指导和标准化方法 分析包括充气、固定、免疫组织化学、激光捕获显微解剖、荧光 原位杂交(FISH)和形态计量学分析。核心有助于精确隔离特定于站点的 血管组织包括显微解剖技术及其表型差异的比较 近端和远端细胞。核心有效地提供了各种各样的新鲜分散和培养的 驻留的血管细胞以标准化的方式分离、生长和表征。可用单元格包括 从啮齿类动物、牛和小鼠的血管平滑肌细胞、内皮细胞和外膜成纤维细胞中提取 人体组织。Core使用免疫荧光染色和细胞类型特异性抗体探针来 记录身份、同质性或异质性的程度以及细胞培养引起的变化 差异化。细胞分选能力可用于提高收获微血管细胞的纯度和得率 人口。Core常规进行体外细胞增殖、毒性、细胞凋亡和迁移分析。 数字成像用于对最初分离后的细胞群体的形态进行分类。 核心协助应用短期和长期的低氧和机械应激刺激新鲜的 分散培养的细胞和使用共培养技术评估潜在的调节作用 邻近的血管细胞。核心通过集中处理以下各项实现规模经济 血管细胞和批量订货。核心部门的其他职责包括：协调 组织培养设备的维护和升级；短期放射性废物管理； 补充库存和维护放射性同位素工作区；持续培训研究员、研究人员和 在染色、成像和细胞培养技术方面的学生工作者。核心便于对人肺的评估 组织通过肺组织研究联盟。

The Tissue Core provides expertise, guidance, and standardized methodology for performing lung structural analysis including inflation, fixation, immunohistochemistry, laser capture micro dissection, fluorescence in situ hybridization (FISH), and morphometric analysis. The Core assists with precise site-specific isolation of vascular tissue including micro-dissection techniques and comparison of phenotypic differences between proximal and distal cells. The Core efficiently provides a wide variety of freshly dispersed and cultured resident vascular cells isolated, grown, and, characterized in a standardized way. Available cells include vascular smooth muscle cells, endothelial cells, and adventitial fibroblasts harvested from rodent, bovine and human tissue. The Core uses immunofluorescent staining with cell-type-specific antibody probes to document identity, extent of homogeneity or heterogeneity, and cell-culture-induced changes in differentiation. Cell sorting capability is available to improve purity and yield of harvested microvascular cell populations. The Core routinely performs in vitro cell proliferation, toxicity, apoptosis, and migration assays. Digital imaging is used to catalogue the morphological appearance of cell populations after initial isolation. The Core assists with application of short and long-term hypoxic and mechanical stress stimulation to freshly dispersed and cultured cells and the use of co-culture techniques to assess the potential modulatory role of neighboring vascular cells. The Core achieves economies of scale through centralized processing of vascular cells and bulk ordering of supplies. Additional responsibilities of the Core include: coordinated maintenance and upgrading of tissue-culture equipment; short-term radioactive waste management; restocking and maintenance of radioisotope work area; and ongoing training of fellows, research staff and student workers in staining, imaging and cell culture techniques. The Core facilitates assess to human lung tissue through the Lung Tissue Research Consortium.