Tissue

Tissue Core提供专业知识、指导和标准化方法， 分析包括膨胀、固定、免疫组织化学、激光捕获显微切割、荧光显微镜下观察和分析。 原位杂交（FISH）和形态测定分析。核心有助于精确的特定地点隔离， 血管组织，包括显微解剖技术和比较表型差异之间 近端和远端细胞。核心有效地提供了各种新鲜分散和培养 常驻血管细胞分离，生长，并以标准化的方式表征。可用的单元格包括 血管平滑肌细胞、内皮细胞和外膜成纤维细胞，从啮齿动物、牛和 人体组织Core使用细胞类型特异性抗体探针进行免疫荧光染色， 记录同一性、同质性或异质性程度以及细胞培养诱导的 分化细胞分选能力可用于提高收获的微血管细胞的纯度和产量 人口。Core常规进行体外细胞增殖、毒性、凋亡和迁移试验。 数字成像用于对初始分离后的细胞群的形态外观进行编目。 核心协助应用短期和长期缺氧和机械应力刺激新鲜 分散和培养的细胞，并使用共培养技术，以评估潜在的调节作用， 邻近的血管细胞核心通过集中处理 血管细胞和批量订购供应品。核心的其他职责包括： 组织培养设备的维护和升级;短期放射性废物管理; 补充库存和维持放射性同位素工作区;不断培训研究员、研究人员和 染色、成像和细胞培养技术方面的学生工作者。核心有助于评估人肺 肺组织研究联合会（Lung Tissue Research Consortium）。

The Tissue Core provides expertise, guidance, and standardized methodology for performing lung structural analysis including inflation, fixation, immunohistochemistry, laser capture micro dissection, fluorescence in situ hybridization (FISH), and morphometric analysis. The Core assists with precise site-specific isolation of vascular tissue including micro-dissection techniques and comparison of phenotypic differences between proximal and distal cells. The Core efficiently provides a wide variety of freshly dispersed and cultured resident vascular cells isolated, grown, and, characterized in a standardized way. Available cells include vascular smooth muscle cells, endothelial cells, and adventitial fibroblasts harvested from rodent, bovine and human tissue. The Core uses immunofluorescent staining with cell-type-specific antibody probes to document identity, extent of homogeneity or heterogeneity, and cell-culture-induced changes in differentiation. Cell sorting capability is available to improve purity and yield of harvested microvascular cell populations. The Core routinely performs in vitro cell proliferation, toxicity, apoptosis, and migration assays. Digital imaging is used to catalogue the morphological appearance of cell populations after initial isolation. The Core assists with application of short and long-term hypoxic and mechanical stress stimulation to freshly dispersed and cultured cells and the use of co-culture techniques to assess the potential modulatory role of neighboring vascular cells. The Core achieves economies of scale through centralized processing of vascular cells and bulk ordering of supplies. Additional responsibilities of the Core include: coordinated maintenance and upgrading of tissue-culture equipment; short-term radioactive waste management; restocking and maintenance of radioisotope work area; and ongoing training of fellows, research staff and student workers in staining, imaging and cell culture techniques. The Core facilitates assess to human lung tissue through the Lung Tissue Research Consortium.