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Tissue

组织

基本信息

批准号：
7662804
负责人：
EDWARD CHARLES DEMPSEY
金额：
$ 25.76万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-07-01 至 2014-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7662804
关键词：
Antibodies Apoptosis Appearance Area Biological Assay Blood Vessels Cataloging Catalogs Cattle Cell Count Cell Culture Techniques Cell Proliferation Cell Separation Cells Cloning Coculture Techniques Culture Techniques Cultured Cells Detection Dissection Distal Economic Inflation Endothelial Cells Equipment Exclusion Fibroblasts Flow Cytometry Fluorescent in Situ Hybridization Future Gene Expression Growth Harvest Heart Heterogeneity Human Hypoxia Image Immunohistochemistry In Vitro Lasers Lung Maintenance Measurement Mechanical Stress Methodology Migration Assay Mitogens Nuclear Performance Population Process Pulmonary Hypertension Radioactive Waste Radioisotopes Research Rodent Role Services Site Smooth Muscle Myocytes Staining method Stains Stimulus Stress Structure of parenchyma of lung Students Techniques Thymidine Tissues Toxic effect Training Training Programs Trypan Blue Waste Management Work cell type digital imaging human tissue improved research study response sample fixation skills tissue culture

项目摘要

The Tissue Core provides expertise, guidance, and standardized methodology for performing lung structural analysis including inflation, fixation, immunohistochemistry, laser capture micro dissection, fluorescence in situ hybridization (FISH), and morphometric analysis. The Core assists with precise site-specific isolation of vascular tissue including micro-dissection techniques and comparison of phenotypic differences between proximal and distal cells. The Core efficiently provides a wide variety of freshly dispersed and cultured resident vascular cells isolated, grown, and, characterized in a standardized way. Available cells include vascular smooth muscle cells, endothelial cells, and adventitial fibroblasts harvested from rodent, bovine and human tissue. The Core uses immunofluorescent staining with cell-type-specific antibody probes to document identity, extent of homogeneity or heterogeneity, and cell-culture-induced changes in differentiation. Cell sorting capability is available to improve purity and yield of harvested microvascular cell populations. The Core routinely performs in vitro cell proliferation, toxicity, apoptosis, and migration assays. Digital imaging is used to catalogue the morphological appearance of cell populations after initial isolation. The Core assists with application of short and long-term hypoxic and mechanical stress stimulation to freshly dispersed and cultured cells and the use of co-culture techniques to assess the potential modulatory role of neighboring vascular cells. The Core achieves economies of scale through centralized processing of vascular cells and bulk ordering of supplies. Additional responsibilities of the Core include: coordinated maintenance and upgrading of tissue-culture equipment; short-term radioactive waste management; restocking and maintenance of radioisotope work area; and ongoing training of fellows, research staff and student workers in staining, imaging and cell culture techniques. The Core facilitates assess to human lung tissue through the Lung Tissue Research Consortium.

组织核心提供专业知识，指导和标准化的方法进行肺结构