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Cellular and Molecular Biology Core

细胞和分子生物学核心

基本信息

批准号：
7647692
负责人：
PATRICIA E GALLAGHER
金额：
$ 17.05万
依托单位：
WAKE FOREST UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7647692
关键词：
1-Phosphatidylinositol 3-Kinase 2-Mercaptoethanol AGTR2 gene APAF1 gene AQP1 gene Acepromazine Actins Adherence Adrenal Glands Affinity Affinity Chromatography Age Air Alcohols Aldosterone Aldosterone Synthase Aliquot American Amino Acids Ampicillin Anesthesia procedures Angiotensin I Angiotensin II Angiotensin II Receptor Angiotensin Receptor Angiotensinogen Angiotensins Animals Annexins Antibiotics Antibodies Antibody Specificity Antifreeze Antigen-Antibody Complex Antigens Antisense Oligonucleotides Apoptosis Apoptotic Appearance Area Astrocytes Astrocytoma Authorization documentation Avidin BIRC5 gene Base Pairing Base Sequence Binding Biological Biological Assay Biology Biomedical Research Biopsy Biopsy Specimen Biotechnology Biotin Blood Blood Vessels Body Weight Brain Breeding Bromodeoxyuridine Buffers C-terminal CCL2 gene CDK4 gene CDKN1A gene CDKN2A gene COS-7 Cell Cancer Center Canis familiaris Carbon Dioxide Cardiac Cardiac Myocytes Caring Catalytic Domain Cattle Cell Count Cell Culture Techniques Cell Extracts Cell Line Cells Cellular biology Cellulose Centrifugation Chest Chinese Hamster Ovary Cell Chloroform Cities Citrates Clinic Collagen Collection Complementary DNA Complex Comprehensive Cancer Center Comprehensive Cancer Center of Wake Forest University Computer Assisted Computer software Connexin 43 Connexins Consultations Contractile Proteins Core Facility Coronary artery Couples Coupling Culture Media Cultured Cells Custom Cyclin-Dependent Kinase Inhibitor Cyclin-Dependent Kinase Inhibitor 2A Cysteine Cytochrome P450 Cytokeratin Cytolysis DNA DUSP1 gene Data Data Analyses Decapitation Decontamination Deoxyribonucleases Deoxyribonucleotides Detection Development Diagnostics Research Diethyl Pyrocarbonate Digestion Dimethyl Sulfoxide Disease Dissociation Documentation Down-Regulation Drops Dry Ice Ear Edetic Acid Effectiveness Electrophoresis Embryo Endopeptidase K Endothelial Cells Ensure Enzyme-Linked Immunosorbent Assay Epidermal Growth Factor Receptor Equus caballus Estrogen Receptor 1 Estrogen Receptor 2 Estrogen Receptors Ethanol Ethylene Glycols Excision Exhibits Exons Extracellular Signal Regulated Kinases Family Family suidae Fees Ferritin Fibroblasts Fibronectins Fixatives Fluorescence Microscopy Fostering Foundations Freezing Freund&apos s Adjuvant G-Protein-Coupled Receptors Gel Gene Expression Gene Expression Regulation Gene Targeting Genes Genetic Status Genetic Transcription Genotype Gifts Glass Glial Fibrillary Acidic Protein Glioma Glycerol Glycine Goat Growth Halothane Heart Heart Hypertrophy Heating Hematoxylin Hematoxylin and Eosin Staining Method Homologous Gene Horseradish Peroxidase Hour Human Human Resources Hydrogen Peroxide Hyperplasia Hypertension Hypertrophy IRS2 gene Image Image Analysis Immunoglobulin G Immunohistochemistry In Vitro Inbred SHR Rats Inbred WKY Rats Incubated Incubators Individual Inflammation Infusion Pumps Infusion procedures Injection of therapeutic agent Instruction Insulin Insulin-Like Growth Factor I Isoflurane Isopropanol KDR gene Ketamine Keyhole Limpet Hemocyanin Kidney Label Laboratories Left ventricular structure Lentivirus Vector Leupeptins Lidocaine Life Lipofectamine Liposomes Liquid substance Literature Liver Local anesthesia Lung MAPK14 gene MAPK8 gene MAS1 gene Magic Manufacturer Name Marshal Measurement Measures Medial Membrane Messenger RNA Metalloproteases Methodology Methods Microscope Microscopy Mineralocorticoid Receptor Mitogen-Activated Protein Kinase 14 Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases Modeling Molecular Molecular Biology Molecular Biology Techniques Molecular Weight Molecular and Cellular Biology Monitor Mouse Protein Mus Muscle Cells Myofibroblast Myosin ATPase NADPH Oxidase NPHS2 protein Names Natriuretic Peptides Needles Neonatal Nephrosis Neprilysin Neuroblastoma Neuroglia Neurons New York Nitric Oxide Synthase Nitric Oxide Synthase Type I Nitrogen North Carolina Nuclear Pore Complex Proteins Oligonucleotides Oncogenes Oryctolagus cuniculus Oxidative Stress PC12 Cells PPP2R5A gene PTEN gene PTGS1 gene PTGS2 gene Pancreas Paraffin Pathway interactions Penicillins Peptide Hydrolases Peptide Receptor Peptides Peptidyl-Dipeptidase A Performance Peroxidases Phenols Phenotype Pheochromocytoma Phosphate Buffer Phosphatidylinositol 4,5-Diphosphate Phospholipase D Phosphopeptides Phosphoric Monoester Hydrolases Phosphotransferases Physiology Placental Growth Factor Plasmids Polylysine Polymerase Polymerase Chain Reaction Polyvinyls Preparation Principal Investigator Procedures Process Production Promega Prostacyclin synthase Prostaglandin Production Prostaglandins Protease Inhibitor Protein Isoforms Protein Phosphatase 2A Regulatory Subunit PR53 Protein Tyrosine Kinase Proteins Proto-Oncogene Proteins c-akt Protocols documentation Public Domains Publications Pump Punch Biopsy RNA RNA Degradation RNA, Ribosomal, 18S RNA-Directed DNA Polymerase Rat Strains Rat-1 Rattus Reaction Reader Reagent Recombinants Reflex action Regulation Relative (related person)Relaxin Renin Reporter Genes Reporting Research Research Personnel Research Project Grants Residual state Retrieval Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Ribonucleases Ribosomal RNA Role Running Saline Sampling Sepharose Series Serpins Serum Serum Proteins Serum-Free Culture Media Services Sheep Side Signal Transduction Signal Transduction Pathway Slice Slide Small Interfering RNA Smooth Muscle Smooth Muscle Actin Staining Method Smooth Muscle Myocytes Sodium Chloride Sodium Dodecyl Sulfate Soluble Guanylate Cyclase Solutions Source South Carolina Specific qualifier value Specificity Spleen Staining method Stains Standard precautions Sterility Streptomycin Structure Subfamily lentivirinae Submandibular gland Surface System TNF-alpha converting enzyme Techniques Technology Temperature Tertiary Protein Structure Testing Thick Thoracic aorta Thrombospondin 1 Thromboxane-A Synthase Thromboxanes Time Tissue Embedding Tissue Fixation Tissues Transfection Transferrin Transgenes Transgenic Animals Transgenic Organisms Treatment Step Triton Triton X100 Trypsin Tube Tubulin Tumor Necrosis Factor-alpha Tumor Necrosis Factors Tunica Adventitia Type 2 Angiotensin II Receptor United States National Institutes of Health Universities Uterus Validation Variant Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factor Receptor Vascular Endothelial Growth Factors Vegetables Vimentin Viral Viral Packaging Viral Vector Virus Water Western Blotting Wit Withdrawal Xiphoid Process Xylene adduct angiotensin I (1-7)ascorbate base caspase-3 caspase-9 cell growth cell type chloramine-T collagenase congenic connective tissue growth factor cost cresyl violet cross reactivity culture plates cyclooxygenase 1 cyclooxygenase 2 cytochrome b245 density design design and construction established cell line ethylene glycol feeding fetal bovine serum flasks fluorescence microscope folin forest glutathione synthase human CDK4 protein human FTH1 protein human IRS2 protein human MAPK14 protein human PTPN1 protein human TGFB1 protein immunocytochemistry inhibitor/antagonist inorganic phosphate interest internal control kidney cell kidney epithelial cell leptin receptor leupeptin mRNA Expression male medical schools myocyte-specific enhancer-binding-factor 2C nano nephrin novel oligofectamine oligonucleotide polymerase oncoprotein p21 overexpression paraform particle polyacrylamide gels polypeptide pressure prevent programs prostaglandin E synthase prostaglandin R2 D-isomerase protein expression pulmonary artery endothelial cell rat KIM-1 protein reagent testing receptor receptor coupling research study rib bone structure scalpel seal small hairpin RNA small molecule stable cell line superoxide dismutase 1 survivin thimet oligopeptidase tissue culture tissue/cell culture tool transduction efficiency trizol vector von Willebrand Factor

项目摘要

The responsibilities of Core B will involve the performance of two separate but related functions: 1) The Tissue Culture unit will provide tissue culture cells and the expertise necessary to complete the cellular biology protocols in the individual proposals and 2) The Molecular Biology unit will provide reagents and expertise to complete the molecular biology experiments described in the five projects. Described below are specific details of the primary methods used in Core B. These include, but are not limited to, reverse transcriptase (RT) polymerase chain reaction (PCR), RT real-time PCR, Northern, Southern and Western blot analysis, development of antisense oligonucleotides and siRNAs, subcloning of gene fragments for riboprobe development, and riboprobe synthesis, and targeted gene array analysis. These procedures will be provided to the investigators during the course of the proposed studies as required and new methodologies will be developed as needed.

核心B的职责将涉及两个独立但相关职能：1）组织培养单位将提供组织培养细胞和必要的专业知识，完成个人提案中的细胞生物学方案，2）分子生物学单元将提供试剂和专业知识，以完成五个项目中描述的分子生物学实验。下文描述了核心B中使用的主要方法的具体细节。这些包括但不限于逆转录酶（RT）聚合酶链反应（PCR）、RT实时PCR、北方、南方和 Western印迹分析、反义寡核苷酸和siRNA的开发、基因片段的亚克隆用于核糖体探针开发、核糖体探针合成和靶向基因阵列分析。这些程序将在拟定研究过程中，根据需要向研究者提供新方法将根据需要进行开发。