Inflammation model of body-based treatment for chronic musculoskeletal pain

慢性肌肉骨骼疼痛的基于身体的治疗的炎症模型

• 批准号: 7804108
• 负责人: Sarah Megan Corey
• 金额: $ 4.22万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-30 至 2011-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7804108
• 关键词: Affect; Animal Model; Animal Rights; Animals; Attenuated; Back; Calcitonin Gene-Related Peptide; Carrageenan; Cell Count; Cells; Chronic; Chronic low back pain; Connective Tissue; Control Animal; Data; Dense Connective Tissue; Development; Enzyme Immunoassay; Evaluation; Fascia; Fast Blue; Goals; Histology; Hour; Human; Hypersensitivity; Immunohistochemistry; Inflammation; Inflammatory; Injection of therapeutic agent; Left; Lifting; Location; Low Back Pain; Lumbar Regions; Manuals; Measures; Mechanics; Methods; Modeling; Movement; Muscle; Musculoskeletal Pain; Nerve Fibers; Nerve Growth Factors; Neurogenic Inflammation; Neuronal Plasticity; Neurons; Neuropeptides; Outcome Measure; Pain; Pathogenesis; Patients; Peripheral; Physical therapy; Play; Production; Proteins; Randomized; Rattus; Role; Sensory; Side; Spinal; Spinal Ganglia; Stretching; Substance P; TNF gene; Tail; Testing; Time; Tissues; Tracer; Ultrasonography; afferent nerve; base; behavior test; conventional therapy; cytokine; in vivo; nerve supply; public health relevance; soft tissue; subcutaneous

DESCRIPTION (provided by applicant): Body-based (manual and movement) therapies have been shown to be beneficial for the treatment of chronic musculoskeletal pain. An important component of manual and movement based therapies is stretching of soft tissues (muscle and connective tissue). A recent ultrasound study in humans suggests that connective tissue may play a role in the pathogenesis of chronic low back pain. Importantly very little is known about the innervation of connective tissues in the low back or how these tissues respond to inflammation. The overall objective of this project is to test the hypothesis that tissue stretch attenuates mechanical sensitivity, cytokine production and neuropeptide production induced by local connective tissue inflammation. The specific Aims of this project are to quantify the neuropeptide expression of sensory nerve fibers projecting from the connective tissues of the low back in the rat (Aim 1), to develop a model of connective tissue inflammation in the low back of the rat (Aim 2a) and to compare the effect of in vivo tissue stretch vs. no stretch in the model of connective tissue inflammation (Aim 2b). To quantify sensory nerve fiber expression we will use retrograde tracer methods to identify the cells that project from the low back in the rat, and immunohlstochemical methods to identify the sensory nerve cells. All cells that project from the low back will be counted and further analysis will involve counting the subpopulation of these cells that also express sensory neuropeptides. To evaluate the effect of tissue stretch on connective tissue inflammation we will apply a method of in vivo animal stretch to a model of connective tissue inflammation in the rat. This inflammatory model and the subsequent changes that may be induced by stretch will be evaluated with behavioral tests for changes in mechanical sensitivity, a pro-inflammatory cytokine array to measure changes in cytokine expression, histology to evaluate inflammation in tissue, enzyme immunoassay to evaluate neuropeptide release in tissue and immunohistochemistry to evaluate neuropeptide expression in dorsal root ganglia. PUBLIC HEALTH RELEVANCE: Chronic musculoskeletal pain including low back pain affects millions of people every year. Stretching of connective tissue and muscle is a fundamental component of body-based (manual and movement) therapies and conventional physical therapy both of which are commonly used to treat persistent and chronic musculoskeletal pain. Body-based therapies are non-invasive and if the peripheral mechanism conferring a decrease in pain and inflammation can be elucidated, treatments could be tailored to effect maximal benefit to the patient.

描述（由申请人提供）：基于身体（手动和运动）的疗法已被证明对慢性肌肉骨骼疼痛的治疗有益。手工和运动疗法的一个重要组成部分是拉伸软组织（肌肉和结缔组织）。最近的一项人体超声研究表明，结缔组织可能在慢性腰痛的发病机制中起作用。重要的是，我们对下背部结缔组织的神经支配以及这些组织对炎症的反应知之甚少。该项目的总体目标是验证组织拉伸减弱局部结缔组织炎症诱导的机械敏感性、细胞因子产生和神经肽产生的假设。本项目的具体目的是量化大鼠下背部结缔组织投射的感觉神经纤维的神经肽表达（Aim 1），建立大鼠下背部结缔组织炎症模型（Aim 2a），比较结缔组织炎症模型中活体组织拉伸与不拉伸的效果（Aim 2b）。为了量化感觉神经纤维的表达，我们将使用逆行示踪方法来识别从下背部投射的细胞