Annotation of the small RNA/microRNA component Drosophila genome

我们的modENCODE母授权U 01-HG 004261旨在注释功能 使用深度测序方法的果蝇短调控RNA。这些 包括已知类型的Argonaute结合RNA，例如微小RNA、小干扰RNA， 和piRNA。此外，由于这一点，可能会发现潜在的新型RNA 项目我们要求行政补充资金，以扩大我们的努力范围， 发现并注释代表或产生短调控RNA的新转录物， 果蝇所要求的大部分资金专门用于支持一个新的 生物信息学家对大量的小RNA数据进行必要的分析， 为财团生产的。我们希望发现编辑过的RNA和物种， 非模板化添加，并分析mi/si/piRNA的非规范基因来源。的 剩余的资金将用于构建和测序的新方法 富集了通常不稳定的初级microRNA转录物的文库。分析应 不仅有助于定义microRNA基因结构，而且可能揭示新类型的 通常不稳定的转录本，可能会逃脱传统的RNA测序的努力。 U 01-HG 004261 - 03 S1 Lai ARRA补充

Our modENCODE parent grant U01-HG004261 is aimed at annotating the functional short regulatory RNAs of Drosophila melanogaster using deep sequencing methodology. These include known classes of Argonaute-bound RNA such as microRNAs, small interfering RNAs, and piRNAs. In addition, potentially novel classes of RNAs may be uncovered as a result of this project. We request administrative supplemental funding to expand the range of our efforts to discover and annotate novel transcripts that represent or generate short regulatory RNAs in Drosophila. The bulk of the requested funds are dedicated to 50% salary support of a new bioinformatician to perform essential analyses of the large amount of small RNA data that we have produced for the consortium. We hope to uncover edited RNAs and species with untemplated additions, and to analyze non-canonical genic sources of mi/si/piRNAs. The remainder of the funds will be devoted towards novel methods for constructing and sequencing libraries that are enriched for normally labile primary microRNA transcripts. Their analysis should not only help to define microRNA gene structures, but may prove to reveal novel types of normally labile transcripts that might escape conventional RNA-seq efforts. U01-HG004261-03S1 Lai ARRA supplement